一种新型火鸡疱疹病毒细菌人工染色体的构建与鉴定

doi:10.11843/j.issn.0366-6964.2016.10.016

Abstract

Abstract:

This study was conducted to construct a novel bacterial artificial chromosome (BAC) of herpevirus of turkey (HVT) as a technical platform for generation of recombinant HVT live vectored vaccine.The mini-F sequences were inserted into the genome of HVT in lieu of glycoprotein C (gC) gene through homologous recombination.The mini-F recombinant HVT were selected by GFP and gpt labeling.Then the DNA of mini-F recombinant HVT was transferred into E.coli DH10B cells to construct the HVT BAC.Following identification of correct construction of HVT whole genome BAC through RFLP method，HVT BAC DNA was transferred into E.coli GS1783 cells for further study after checking again.Then the HVT BAC was transfected into chicken embryo fibroblasts (CEF) for rescuing of virus.And also the gC recovered virus was generated by replacement of mini-F sequences with gC gene through homologous recombination again.Finally，the growth characteristics and protective efficacy against Marek’s disease of the gC recovered HVT，mini-F recombinant HVT and the parental virus (HVT FC126) were investigated.Five strains of mini-F recombinant HVT was obtained.One of the five recombinants (HVT^mini-F/ΔgC) was selected for isolation of DNA to transfer into E.coli DH10B cells to construct HVT BAC which was identified successfully through RFLP.Following up，DNA of HVT BAC was transferred into E.coli GS1783 cells successfully to obtain several positive clones and one of these clones was selected and named BAC^HVT-G.The recombinant HVT was rescued successfully from BAC^HVT-G，named HVT^BAC-ΔgC.And the gC recovered virus(HVT^BAC-gC-R) was successfully obtained through homologous recombination.The HVT^BAC-gC-R and HVT FC126 showed no significant difference for growth kinetics or immunity.However，the propagation ability of HVT^BAC-ΔgC was significantly decreased compared to HVT and the immunity against MD of HVT^BAC-ΔgC was also decreased.This study successfully constructed a novel HVT bacterial artificial chromosome，which was an infectious clone of the whole genome of HVT.The gC gene of HVT is a non-essential gene，but plays an important role for propagation of virus.The deletion of gC gene will reduce the immunity of HVT against Marek’s disease.

CLC Number:

S852.659.1

WANG Zhi-sheng，LIU Fang，XU Meng-wei，QIAO Yong-feng，HOU Ji-bo，WANG Ji-chun. Construction and Identification of a Novel Bacterial Artificial Chromosome of Herpesvirus of Turkey[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2071-2080.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/ 10.11843/j.issn.0366-6964.2016.10.016

https://www.xmsyxb.com/EN/Y2016/V47/I10/2071

References

［1］MELCHIOR F J，FREDRICKSON T，LUGINBUHL R.Neutralization studies with Marek’s disease virus and turkey herpesvirus［J］.Appl Microbiol，1973，26(6)：925-933.
［2］RAHAUS M，AUGUSTINSKI K，CASTELLS M，et al.Application of a new bivalent Marek’s disease vaccine does not interfere with infectious bronchitis or Newcastle disease vaccinations and proves efficacious［J］.Avian Dis，2013，57(2 Suppl)：498-502.
［3］AFONSO C，TULMAN E，LU Z，et al.The genome of turkey herpesvirus［J］.J Virol，2001，75(2)：971-978.
［4］SONDERMEIJER P，CLAESSENS J，JENNISKENS P，et al.Avian herpesvirus as a live viral vector for the expression of heterologous antigens［J］.Vaccine，1993，11(3)：349-358.
［5］TSUKAMOTO K，SAITO S，SAEKI S，et al.Complete，long-lasting protection against lethal infectious bursal disease virus challenge by a single vaccination with an avian herpesvirus vector expressing VP2 antigens［J］.J Virol，2002，76(11)：5637-5645.
［6］DARTEIL R，BUBLOT M，LAPLACE E，et al.Herpesvirus of turkey recombinant viruses expressing infectious bursal disease virus (IBDV) VP2 immunogen induce protection against an IBDV virulent challenge in chickens［J］.Virology，1995，211(2)：481-490.
［7］ESAKI M，GODOY A，ROSENBERGER J，et al.Protection and antibody response caused by turkey herpesvirus vector Newcastle disease vaccine［J］.Avian Dis，2013，57(4)，750-755.
［8］PALYA V，KISS I，TATÁR-KIS T，et al.Advancement in vaccination against Newcastle disease：recombinant HVT NDV provides high clinical protection and reduces challenge virus shedding with the absence of vaccine reactions［J］.Avian Dis，2012，56(2)：282-287.
［9］RAUW F，PALYA V，GARDIN Y，et al.Efficacy of rHVT-AI vector vaccine in broilers with passive immunity against challenge with two antigenically divergent Egyptian clade 2.2.1 HPAI H5N1 strains［J］.Avian Dis，2012，56(4 Suppl)：913-922.
［10］PALYA V，TATÁR-KIS T，MATÓ T，et al.Onset and long-term duration of immunity provided by a single vaccination with a turkey herpesvirus vector ND vaccine in commercial layers［J］.Vet Immunol Immunopathol，2014，158(1-2)：105-115.
［11］LIU S，SUN W，CHU J，et al.Construction of recombinant HVT expressing PmpD，and immunological evaluation against chlamydia psittaci and Marek’s disease virus［J］.PLoS One，2015，10(4)：e0124992.
［12］PANTIN-JACKWOOD M，KAPCZYNSKI D，DEJESUS E，et al.Efficacy of a recombinant turkey herpesvirus h5 vaccine against challenge With H5N1 clades 1.1.2 and 2.3.2.1 highly pathogenic avian influenza viruses in domestic ducks (Anas platyrhynchos domesticus)［J］.Avian Dis，2016，60(1)：22-32.
［13］LIU J，CHEN P，JIANG Y，et al.A duck enteritis virus-vectored bivalent live vaccine provides fast and complete protection against H5N1 avian influenza virus in ducks［J］.J Virol，2011，85(21)：10989-10998.
［14］CHEN Y，GUO W，XU Z，et al.A novel recombinant pseudorabies virus expressing parvovirus VP2 gene：Immunogenicity and protective efficacy in swine［J］.Virol J，2011，8：307.
［15］兰德松，石星明，王云峰，等. 火鸡疱疹病毒细菌人工染色体的构建［J］.微生物学报，2008，48（6）：811-817.
LAN D S，SHI X M，WANG Y F，et al.Reconstituting turkey herpesvirus with bacterial artificial chromosome clones［J］.Acta Microbiologica Sinica，2008，48(6)：811-817.（in Chinese）
［16］WARDEN C，TANG Q，ZHU H.Herpesvirus BACs：past，present，and future［J］.J Biomed Biotechnol，2011：124595.
［17］TISCHER B，SMITH G，OSTERRIEDER N.En passant mutagenesis：a two step markerless red recombination system［J］.Methods Mol Biol，2010，634：421-430.
［18］OSTERRIEDER N，SCHUMACHER D，TRAPP S，et al.Establishment and use of infectious bacterial artificial chromosome (BAC) DNA clones of animal herpesviruses［J］.Berl Munch Tierarztl Wochenschr，2003，116：373-380.
［19］MESSERLE M，CRNKOVIC I，HAMMERSCHMIDT W，et al.Cloning and mutagenesis of a herpesvirus genome as an infectious bacterial artificial chromosome［J］.Proc Natl Acad Sci U S A，1997，94：14759-14763.
［20］OSTERRIEDER N.Construction and characterization of an equine herpesvirus 1 glycoprotein C negative mutant［J］.Virus Res，1999，59：165-77.
［21］WANG J，OSTERRIEDER N.Generation of an infectious clone of duck enteritis virus and generation of a vectored DEV expressing hemagglutinin of H5N1 avian influenza virus［J］.Virus Res，2011，159(1)：23-31.
［22］TISCHER B，VON EINEM J，KAUFER B，et al.Two-step red-mediated recombination for versatile high-efficiency markerless DNA manipulation in Escherichia coli［J］.Biotechniques，2006，40：191-197.
［23］REDDY S，SUN A，KHAN O，et al.Cloning of a very virulent plus，686 strain of Marek’s disease virus as a bacterial artificial chromosome［J］.Avian Dis，2013，57(2 Suppl)：469-473.
［24］WANG J，GE A，XU M，et al.Construction of a recombinant duck enteritis virus (DEV) expressing hemagglutinin of H5N1 avian influenza virus based on an infectious clone of DEV vaccine strain and evaluation of its efficacy in ducks and chickens［J］.Virol J，2015，12：126.
［25］MA C，ZHANG Z，ZHAO P，et al.Comparative transcriptional activity of five promoters in BAC-cloned MDV for the expression of the hemagglutinin gene of H9N2 avian influenza virus［J］.J Virol Methods，2014，206：119-127.
［26］BAIGENT S，PETHERBRIDGE L，SMITH L，et al.Herpesvirus of turkey reconstituted from bacterial artificial chromosome clones induces protection against Marek’s disease［J］.J Gen Virol，2006，87(Pt 4)：769-776.
［27］HALL R，MEERS J，FOWLER E，et al.Identification of non-essential loci within the Meleagrid herpesvirus 1 genome［J］.Virol J，2015，12：130.
［28］RAUW F，GARDIN Y，PALYA V，et al.The combination of attenuated Newcastle disease (ND) vaccine with rHVT-ND vaccine at 1 day old is more protective against ND virus challenge than when combined with inactivated ND vaccine［J］.Avian Pathol，2014，43(1)：26-36.
［29］PRANDINI F，SIMON B，JUNG A，et al.Comparison of infectious bursal disease live vaccines and a HVT-IBD vector vaccine and their effects on the immune system of commercial layer pullets［J］.Avian Pathol，2016，45(1)：114-125.
［30］KAPCZYNSKI D，ESAKI M，DORSEY K，et al.Vaccine protection of chickens against antigenically diverse H5 highly pathogenic avian influenza isolates with a live HVT vector vaccine expressing the influenza hemagglutinin gene derived from a clade 2.2 avian influenza virus［J］.Vaccine，2015，33(9)：1197-1205.
［31］ADAMIAK B，TRYBALA E，MARDBERG K，et al.Human antibodies to herpes simplex virus type 1 glycoprotein C are neutralizing and target the heparan sulfate-binding domain［J］.Virology，2010，400：197-206.
［32］JAROSINSKI K，OSTERRIEDER N.Further analysis of Marek’s disease virus horizontal transmission confirms that U(L)44 (gC) and U(L)13 protein kinase activity are essential，while U(S)2 is nonessential［J］.J Virol，2010，84：7911-7916.

[1]	CHI Lili, WANG Junna, ZHANG Yuming, LIU Jian, CHEN Zhiyuan, LI Shufan, YIN Yanbo, XU Shouzhen. Prokaryotic Expression and Immunogenicity Analysis of Truncated Fusion Protein of FAdV-4 Fiber2 and FAdV-8b Fiber [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5162-5170.
[2]	LI Linyan, TENG Man, LIU Jinling, ZHENG Luping, CHAI Shujun, DING Ke, YU Zuhua, LUO Jun. Development of a Monoclonal Antibody Pool for Marek’s Disease Virus and Identification of a Specific mAb against the Glycoprotein gB [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4712-4723.
[3]	ZHANG Fangyuan, YANG Dawei, QIU Deyang, JIANG Guoqian, LI Guimei, SHAN Hu. Expression of ASFV P30 Protein and Development of ASFV Antibody Detection Method Based on x-MAP Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4300-4310.
[4]	FENG Yongzhi, GONG Ting, WU Dongdong, GAO Qi, ZHENG Xiaoyu, ZHANG Guihong, SUN Yankuo. Analysis of Factors Affecting the Infectivity of African Swine Fever Virus on Cultured Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3406-3414.
[5]	ZHANG Yi, WANG Chenyan, YANG Xiao, SHAO Guoqing, HOU Bo. Phylogenetic Analysis and Pathogenicity of an Avian Infectious Laryngotracheitis Virus Strain WF03 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3435-3444.
[6]	DING Xiaoyan, HE Jiuxiang, ZHOU Xiaoyang, ZHOU Yuxin, LI Jintao. Preliminary Identification of Host Regulatory Genes and Virulence Genes during African Swine Fever Virus Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2964-2971.
[7]	CHEN Hongjian, CAO Yan, FAN Jie, GAN Rongxuan, SONG Wenbo, YU Shengwei, YANG Ting, ZHAO Yanxia, WEI Chunyan, XIE Rui, HUA Lin, PENG Zhong, WU Bin. Prevalence and Phylogenetic Analysis of Pseudorabies Virus within Pig Slaughterhouses in Hubei Province of China during 2020-2022 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2972-2981.
[8]	WANG Jiali, ZHOU Ning, CHEN Xi, YUE Hua, TANG Cheng. Isolation, Identification and Pathogenicity of Canine Adenovirus Type 2 Strain with Natural Deletion in E3 Gene [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2982-2990.
[9]	FENG Weimin, LIU Xiao, HUANG Teng. The Evasion Strategy against CTL Recognition by Herpesviruses of Domestic Animals: Interference with MHC Class Ⅰ Antigen Presentation Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2241-2251.
[10]	GAO Zhenzhen, MENG Zejing, HE Xiaobing, FANG Yongxiang, TIAN Huihui, CHEN Guohua, JING Zhizhong. Identification of Immunogenicity and Neutralizing Activity of Ectromelia Virus EVM135 and EVM085 Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2468-2477.
[11]	WANG Ying, ZHU Jiahong, ZHAO Jiakai, JI Pinpin, CHEN Xu, ZHANG Lu, LIU Baoyuan, SUN Yani, ZHAO Qin. Screening and Identification of Nanobodies against NP419L Protein of African Swine Fever Virus and Its Preliminary Application of Antibody Detection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2509-2520.
[12]	QIU Yingwu, CHANG Hao, PENG Jie, YI Heyou, WANG Qiumei, GUO Yanchen, WU Qianwen, CAO Xuezhen, LIN Limiao, LI Wei, ZHOU Qingfeng, ZHANG Guihong, LI Qunhui, GONG Lang. A Real-time PCR Method for the Rapid Detection of African Swine Fever Virus I177L Gene Deletion Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2521-2527.
[13]	LONG Qinqin, WEI Min, WANG Yuting, WEN Ming, PANG Feng. The Battle between Orf Virus and Host: Immune Response and Viral Immune Evasion Mechanisms [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1845-1853.
[14]	WANG Guochao, ZHAO Yaru, ZHANG Zhonghui, ZHANG Yulong, BAI Ge, GENG Shuxian, FAN Jie, YANG Jifei, GUAN Guiquan, YIN Hong, LUO Jianxun, NIU Qingli. Bioinformatics Analysis of RNA Polymerase Subunit D205R Gene of African Swine Fever Virus and Polyclonal Antibody Preparation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2042-2049.
[15]	YUAN Sheng, LI Anqi, Lü Wenke, YANG Lulu, ZHOU Feng, HUANG Liangzong, BAI Aiquan, WEN Feng, HUANG Shujian, GUO Jinyue. Analysis of Variation in Major Virulence-related Genes of a Strain of Pseudorabies Virus and Its Pathogenicity to Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2195-2199.

Construction and Identification of a Novel Bacterial Artificial Chromosome of Herpesvirus of Turkey

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments