Table1

Primer sequence"

Fig.1

Venny diagram of the common target of berberine and S. aureus"

Fig.2

GO functional enrichment analysis of core targets"

Fig.3

Bubble diagram of KEGG pathway enrichment analysis for core target"

Fig.4

PPI network of berberine and Staphylococcus aureus targets"

Fig.5

PPI core target network diagramRed nodes. Potentially key regulatory proteins or molecules that play a central role in the onset and progression of disease. Red nodes in the figure include JUN， CASP3， SRC， and ESR1. Orange nodes. These nodes represent targets directly connected to red nodes. They may be slightly less important than red nodes in the network， but they are still important interaction partners. In the figure， orange nodes include TP53， CDK4， CCNA2， and CDKN1A. Yellow nodes. These nodes may represent other important targets in the network， potentially playing roles in specific biological processes or having indirect interactions with red and orange nodes. In the figure， yellow nodes include ANXA5， CDK1， and CDKN1A"

Table 2

Molecular docking information"

Fig. 6

Molecular docking resultsA. Hydrophobic interactions between BBR molecules and Tp53 protein. B. Hydrophobic interactions between BBR molecules and Casp3 protein. C. Hydrophobic interactions between BBR molecules and Cdkn1a protein. D. Hydrophobic interactions between BBR molecules and Esr1 protein. E. Hydrophobic interactions between BBR molecules and Jun protein. F. Hydrophobic interactions between BBR molecules and Src protein."

Table 3

Body mass and organ index of mice （x±s）"

Fig.7

Pathological section of mouse liver （Bar=50 μm）A. Control group （NC）； B. Model control group （MC）； C. BBR-treated model control group （BBR-MC）"

Fig.8

Transcription quantity of core target genes in mice liver*. P<0.05， indicates a statistically significant difference； **. P<0.01， indicates a more pronounced difference； ***. P<0.001， indicates a significant difference； ****. P<0.001， indicates a highly significant difference； NS. Denotes non-significant. The same as below"

Fig.9

Pathological section of mouse spleen （200 μm）A. Control group （NC）； B. Model control group （MC）； C. BBR-treated model control group （BBR-MC）"

Fig.10

Transcription quantity of core target gene in mice spleen"