金黄色葡萄球菌脂蛋白对奶牛骨髓源巨噬细胞炎症介质分泌及前列腺素E2合成分泌的影响

doi:10.11843/j.issn.0366-6964.2025.07.039

Abstract

Abstract:

Staphylococcus aureus (S. aureus) triggers an inflammatory response in the host upon invading mammalian systems, but the role of its lipoproteins in cytokine release and PGE₂ synthesis remains unclear. This study used the S. aureus SA113 strain, its lipoprotein-deficient mutant, and the complemented strain to infect bovine bone marrow-derived macrophages (bBMMs), aiming to investigate the role of S. aureus lipoproteins in stimulating cytokine release and PGE₂ production in immune cells. In this study, the mechanism of action of S. aureus lipoprotein in inducing the secretion and synthesis of immune cell inflammatory mediators and PGE₂ by infecting bovine bone marrow-derived macrophages with SA113, S. aureus SA113 isogenic mutant lgt: : ermB (Δlgt) deficient in lipoprotein maturation, and its complemented strain SA113 lgt: : ermB +pRBlgt (+pRB). The results showed that compared to the SA113-infected group, the secretion level of PGE₂ significantly decreased after infecting bBMMs with the S. aureus Δlgt mutant, while the secretion levels of pro-inflammatory cytokines (TNF-α and IL-1β) and anti-inflammatory cytokine (IL-10) also significantly decreased. Western blot results indicated that S. aureus lipoprotein influenced the activation of the NF-κB/MAPK pathway in bBMMs and the expression of PGE₂ synthetic enzymes COX-2 and mPGES-1. In addition, the expression levels of TLR2, TLR4 and NLRP3 genes in bBMMs were significantly reduced after loss of S. aureus lipoprotein. In conclusion, during S. aureus infection, S. aureus lipoprotein plays a significant role in activating macrophage inflammatory signaling pathways, mediating the secretion of inflammatory mediators, and it is closely associated with the synthesis and secretion of PGE₂. This study offers a theoretical foundation for the clinical use of prostaglandin synthetase inhibitors and non-steroidal anti-inflammatory drugs (NSAIDs) to prevent and treat infections from S. aureus.

Key words: Staphylococcus aureus, bovine bone marrow-derived macrophages, prostaglandin E2, lipoprotein, cytokines

CLC Number:

S859.7

LIU Yuze, YU Zhuoya, GONG Zhiguo, REN Peipei, ZHAO Jiamin, MAO Wei, ZHANG Shuangyi, FENG Shuang. The Impact of Lipoprotein on the Secretion of Inflammatory Mediators and the Synthesis of Prostaglandin E2 in Bovine Bone Marrow-derived Macrophages Infected with Staphylococcus aureus[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3474-3483.

Figures/Tables 7

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References 30

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引物名称 Primer name	GenBank登录号 GenBank accession No	引物序列 Primer sequences
β-actin	NM_173979.3	Forward 5′-TCACCAACTGGGACGACA-3′
		Reverse 5′-GCATACAGGGACAGCACA-3′
TLR2	NM_174197.2	Forward 5′-CGATGACTACCGCTGTGACTC-3′
		Reverse 5′-CCTTCCTGGGCTTCCTCTT-3′
TLR4	NM_174198.6	Forward: 5′-TGCCTTCACTACAGGGACTTT-3′
		Reverse: 5′-TGGGACACCACGACAATAAC-3′
NLRP3	NM_001102219.1	Forward: 5′-CAGATGAGCAGCAAGCAAGG-3′
		Reverse: 5′-ACAATCCAGCAGACCAGAGG-3′
COX-2	XM_007115297.3	Forward: 5′-GGTGCCTGGTCTGATGATGT-3′
		Reverse: 5′-GATTAGCCTGCTTGTCTGGAAC-3′
mPGES-1	XM_027556544.1	Forward: 5′-ATGGTACACACCGTGGCATA-3′
		Reverse: 5′-CACAATCTCAAAGGGCCATC-3′

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The Impact of Lipoprotein on the Secretion of Inflammatory Mediators and the Synthesis of Prostaglandin E2 in Bovine Bone Marrow-derived Macrophages Infected with Staphylococcus aureus

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