常山散对感染不同发育阶段球虫雏鸡盲肠机械屏障的影响

doi:10.11843/j.issn.0366-6964.2026.01.046

Abstract

Abstract:

The aim of this study was to investigate the effect of Radix dichroapowder （RDP） on intestinal mechanical barrier at different developmental stages of chicks infected with coccidia. The experiment was divided into 6 groups， which were administered on the day before the challenge， the day of the attack， and the 2nd， 3rd， 4th and 5th days after the attack.Each experimental group included Group I （Healthy control group）， Group Ⅱ （Infection control group） and Group Ⅲ （RDP recommended dose group）. At the end of the administration， the chick cecum of each group were collected to make pathological sections. The immunohistochemical analysis of intestinal tight junction proteins occludin and claudin-1 were performed by Pro PLUS 6.0 image software， and the secretion of intestinal goblet cells and mucin were determined.The results showed that compared with Group Ⅰ， the expression levels of occludin protein， claudin-1 protein， and intestinal mucin in Group Ⅱ and Group Ⅲ were significantly reduced （P<0.05）， and the number of goblet cells was significantly decreased （P<0.05）. Compared with Group Ⅱ， the expression of the above three proteins of Group Ⅲ were significantly improved （P<0.05）， and the number of goblet cells were increased from the day before the challenge to 3 days after the challenge. However， with the continuous development of coccidia in chicks and the delay in administration time， the above three proteins in Group Ⅱ and Group Ⅲ were markedly decreased on the 4th and 5th day of challenge （P>0.05）， and the goblet cells disapeared.In summary， RDP can reduce the damage of coccidia to the host intestinal tract， improve the secretion of intestinal barrier protein， and reduce the damage of coccidia to the intestinal structure； the earlier the drug is administered after the challenge， the better the improvement of intestinal damage caused by coccidia.

Key words: Radix dichroa powder, chicks, coccidia, intestine, mechanical barrier

CLC Number:

S859.795

GUO Zhiting, SUN Huanhuan, LIU Shuning, LI Jianxi, ZHANG Shaobo, LIU Yuan, LUO Xiaoqin, WEI Xiaocheng, LI Chengyi. Effect of Radix dichroa Powder on Intestinal Mechanical Barrier of Chicks Infected with Coccidia at Different Developmental Stages[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 526-539.

Figures/Tables 9

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References 31

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项目 Item	组别 Group	动物数 Animal numbers	子孢子接种量 Subspore inoculum volume	饲料中药物浓度 Drug concentration in feed	给药时间/d Days of administration
攻虫前1天给药 Dosing 1d before attack	Ⅰ	10	—	—	—
	Ⅱ	10	5×10⁴·只^-1	—	—
	Ⅲ	10	5×10⁴·只^-1	常山散（0.1 g·kg^-1）	连续给药4 d
攻虫当天给药 Dosing on the day of attack	Ⅰ	10	—	—	—
	Ⅱ	10	5×10⁴·只^-1	—	—
	Ⅲ	10	5×10⁴·只^-1	常山散（0.1 g·kg^-1）	连续给药4 d
攻虫后第2天给药 Dosing on the 2nd day after the attack	Ⅰ	10	—	—	—
	Ⅱ	10	5×10⁴·只^-1	—	—
	Ⅲ	10	5×10⁴·只^-1	常山散（0.1 g·kg^-1）	连续给药4 d
攻虫后第3天给药 Dosing on the 3d day after the attack	Ⅰ	10	—	—	—
	Ⅱ	10	5×10⁴·只^-1	—	—
	Ⅲ	10	5×10⁴·只^-1	常山散（0.1 g·kg^-1）	连续给药4 d
攻虫后第4天给药 Dosing on the 4th day after the attack	Ⅰ	10	—	—	—
	Ⅱ	10	5×10⁴·只^-1	—	—
	Ⅲ	10	5×10⁴·只^-1	常山散（0.1 g·kg^-1）	连续给药4 d
攻虫后第5天给药 Dosing on the 5th day after the attack	Ⅰ	10	—	—	—
	Ⅱ	10	5×10⁴·只^-1	—	—
	Ⅲ	10	5×10⁴·只^-1	常山散（0.1 g·kg^-1）	连续给药4 d

Effect of Radix dichroa Powder on Intestinal Mechanical Barrier of Chicks Infected with Coccidia at Different Developmental Stages

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