家畜囊胚质量评价指标的研究进展与前沿探索

doi:10.11843/j.issn.0366-6964.2026.01.003

Abstract

Abstract:

The evaluation of blastocyst quality is of great significance for assisted reproductive technology （ART）. Through quality assessment， blastocysts with the highest implantation potential can be selected， reducing the number of embryo transfers and improving the clinical pregnancy rate. However， there are many methods for blastocyst quality evaluation， but there is a lack of horizontal and systematic comparison. At present， the existing blastocyst quality evaluation methods mainly include morphological evaluation， metabolomics， time-lapse monitoring technology， genetic screening technology， artificial intelligence technology， etc. This paper systematically describes and compares the most effective and widely used methods for evaluating the quality of blastocysts at the blastocyst stage by summarizing the existing research systems and comprehensively discussing the advantages and limitations of various blastocyst quality evaluation technologies. Meanwhile， optimization schemes are proposed according to the limitations of various methods. It provides a theoretical basis and technical support for further optimizing the blastocyst quality evaluation technology， improving the clinical pregnancy rate and enhancing the efficiency of embryo selection.

Key words: blastocyst stage embryo, quality evaluation, frontier exploration, research progress

CLC Number:

S814

WANG Yanbo, ZHANG Xiaomeng, FENG Xiaoyi, SONG Haoran, DONG Jianhua, PAN Hongmei, ZHAO Xueming. Research Progress and Frontier Exploration of Quality Evaluation Indicators for Domestic Animal Blastocysts[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 22-29.

Figures/Tables 1

References 69

[1]	RIVRON N C，MARTINEZ ARIAS A，PERA M F，et al.An ethical framework for human embryology with embryo models［J］.Cell，2023，186（17）：3548-3557.
[2]	FONSECA J F，OLIVEIRA M E F，BRANDÃO F Z，et al.Non-surgical embryo transfer in goats and sheep：the Brazilian experience［J］.Reprod Fertil Dev，2018，31（1）：17-26.
[3]	SIVANANTHAM S，SARAVANAN M，SHARMA N，et al.Morphology of inner cell mass：a better predictive biomarker of blastocyst viability［J］.Peer J，2022，10：e13935.
[4]	PINTO S，GUERRA-CARVALHO B，CRISOSTOMO L，et al.Metabolomics integration in assisted reproductive technologies for enhanced embryo selection beyond morphokinetic analysis［J］.Int J Mol Sci，2024，25（1）：491.
[5]	GARDNER D K，LANE M，STEVENS J，et al.Blastocyst score affects implantation and pregnancy outcome：towards a single blastocyst transfer［J］.Fertil Steril，2000，73（6）：1155-1158.
[6]	AHLSTRÖM A，WESTIN C，REISMER E，et al.Trophectoderm morphology：an important parameter for predicting live birth after single blastocyst transfer［J］.Hum Reprod，2011，26（12）：3289-3296.
[7]	GARDNER D K，LANE M.Culture and selection of viable blastocysts：a feasible proposition for human IVF？［J］.Hum Reprod Update，1997，3（4）：367-382.
[8]	ZHAO Y Y，YU Y，ZHANG X W.Overall blastocyst quality，trophectoderm grade，and inner cell mass grade predict pregnancy outcome in euploid blastocyst transfer cycles［J］.Chin Med J （Engl），2018，131（11）：1261-1267.
[9]	ALMAGOR M，HARIR Y，FIELDUST S，et al.Ratio between inner cell mass diameter and blastocyst diameter is correlated with successful pregnancy outcomes of single blastocyst transfers［J］.Fertil Steril，2016，106（6）：1386-1391.
[10]	Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology.The Istanbul consensus workshop on embryo assessment：proceedings of an expert meeting［J］.Hum Reprod，2011，26（6）：1270-1283.
[11]	ANAGNOSTOPOULOU C，MALDONADO ROSAS I，SINGH N，et al.Oocyte quality and embryo selection strategies：a review for the embryologists，by the embryologists［J］.Panminerva Med，2022，64（2）：171-184.
[12]	HARDARSON T，VAN LANDUYT L，JONES G.The blastocyst［J］.Hum Reprod，2012，27（Suppl）：1i72-91.
[13]	HILL M J，RICHTER K S，HEITMANN R J，et al.Trophectoderm grade predicts outcomes of single-blastocyst transfers［J］.Fertil Steril，2013，99（5）：1283-1289.e1281.
[14]	GARDNER D K，SURREY E，MINJAREZ D，et al.Single blastocyst transfer：a prospective randomized trial［J］.Fertil Steril，2004，81（3）：551-555.
[15]	FRAGOULI E，ALFARAWATI S，SPATH K，et al.Morphological and cytogenetic assessment of cleavage and blastocyst stage embryos［J］.Mol Hum Reprod，2014，20（2）：117-126.
[16]	SAKKAS D，GARDNER D K.Noninvasive methods to assess embryo quality［J］.Currt Opin Obst Gynecol，2005，17（3）：283-288.
[17]	ZANINOVIC N，ROSENWAKS Z.Artificial intelligence in human in vitro fertilization and embryology［J］.Fertil Steril，2020，114（5）：914-920.
[18]	ALFARAWATI S，FRAGOULI E，COLLS P，et al.The relationship between blastocyst morphology，chromosomal abnormality，and embryo gender［J］.Fertil Steril，2011，95（2）：520-524.
[19]	SINCLAIR K D，LEA R G，REES W D，et al.The developmental origins of health and disease：current theories and epigenetic mechanisms［J］.Soc Reprod Fertil Suppl，2007，64425-64443.
[20]	ALEGRE L，DEL GALLEGO R，ARRONES S，et al.Novel noninvasive embryo selection algorithm combining time-lapse morphokinetics and oxidative status of the spent embryo culture medium［J］.Fertil Steril，2019，111（5）：918-927.e913.
[21]	PUDAKALAKATTI S M，UPPANGALA S，D'SOUZA F，et al.NMR studies of preimplantation embryo metabolism in human assisted reproductive techniques：a new biomarker for assessment of embryo implantation potential［J］.NMR Biomed，2013，26（1）：20-27.
[22]	DONNAY I，PARTRIDGE R J，LEESE H J.Can embryo metabolism be used for selecting bovine embryos before transfer？［J］.Reprod Nutr Dev，1999，39（5-6）：523-533.
[23]	NEL-THEMAAT L，NAGY Z P.A review of the promises and pitfalls of oocyte and embryo metabolomics［J］.Placenta，2011，32 ：S257-263.
[24]	MIAO S B，FENG Y R，WANG X D，et al.Glutamine as a potential noninvasive biomarker for human embryo selection［J］.Reprod Sci，2022，29（6）：1721-1729.
[25]	INOUE N，NISHIDA Y，HARADA E，et al.GC-MS/MS analysis of metabolites derived from a single human blastocyst［J］.Metabolomics，2021，17（2）：17.
[26]	TSOPP E，KILK K，GAMBINI A，et al.Metabolomic biomarkers in bovine embryo culture media and their relationship with the developmental potential of in vitro-produced embryos［J］.Int J Mol Sci，2025，26（5）：2362.
[27]	TSOPP E，KILK K，TAALBERG E，et al.Associations of the single bovine embryo growth media metabolome with successful pregnancy［J］.Metabolites，2024，14（2）：89.
[28]	LANE M，GARDNER D K.Selection of viable mouse blastocysts prior to transfer using a metabolic criterion［J］.Hum Reprod，1996，11（9）：1975-1978.
[29]	PINTO S，GUERRA-CARVALHO B，CRISóSTOMO L，et al.Metabolomics integration in assisted reproductive technologies for enhanced embryo selection beyond morphokinetic analysis［J］.Int J Mol Sci，2023，25（1）：491.
[30]	SIRISTATIDIS C，DAFOPOULOS K，PAPAPANOU M，et al.Why has metabolomics so far not managed to efficiently contribute to the improvement of assisted reproduction outcomes？ The answer through a review of the best available current evidence［J］.Diagnostics （Basel），2021，11（9）：1602.
[31]	LEUNG A S，SON W Y，DAHAN M H.Time-lapse imaging of embryos：current evidence supporting its use［J］.Expert Rev Med Devices，2016，13（10）：881-883.
[32]	MAGATA F.Time-lapse monitoring technologies for the selection of bovine in vitro fertilized embryos with high implantation potential［J］.J Reprod Dev，2023，69（2）：57-64.
[33]	LIU H J，OH S，TAY N L，et al.Morphokinetic analyses of fishing cat-domestic cat interspecies somatic cell nuclear transfer embryos through a time-lapse system［J］.Animals （Basel），2025，15（2）：148.
[34]	BODRI D，SUGIMOTO T，SERNA J Y，et al.Influence of different oocyte insemination techniques on early and late morphokinetic parameters：retrospective analysis of 500 time-lapse monitored blastocysts［J］.Fertil Steril，2015，104（5）：1175-1181.e1171-1172.
[35]	MALLOL A，PIQUÉ L，SANTALÓ J，et al.Morphokinetics of cloned mouse embryos treated with epigenetic drugs and blastocyst prediction［J］.Reproduction，2016，151（3）：203-214.
[36]	YAACOBI-ARTZI S，KALO D，ROTH Z.Seasonal variation in the morphokinetics of in-vitro-derived bovine embryos is associated with the blastocyst developmental competence and gene expression［J］.Front Reprod Health，2022，Nov 3：4：1030949.
[37]	MAGATA F，URAKAWA M，MATSUDA F，et al.Developmental kinetics and viability of bovine embryos produced in vitro with sex-sorted semen［J］.Theriogenology，2021，161：243-251.
[38]	HARADA Y，KINUTANI M，HORIUCHI T.Time-lapse monitoring of mouse embryos produced by injecting sonicated，frozen-thawed sperm heads with high or low chromosomal integrity［J］.Reprod Med Biol，2020，19（2）：171-177.
[39]	PARK J K，JEON Y，BANG S，et al.Time-lapse imaging of morula compaction for selecting high-quality blastocysts：a retrospective cohort study［J］.Arch Gynecol Obstet，2024，309（6）：2897-2906.
[40]	APTER S，EBNER T，FREOUR T，et al.Good practice recommendations for the use of time-lapse technology（†）［J］.Hum Reprod Open，2020，2020（2）：hoaa008.
[41]	LUNDIN K，PARK H.Time-lapse technology for embryo culture and selection［J］.Ups J Med Sci，2020，125（2）：77-84.
[42]	DESAI N，GOLDBERG J M，AUSTIN C，et al.Are cleavage anomalies，multinucleation，or specific cell cycle kinetics observed with time-lapse imaging predictive of embryo developmental capacity or ploidy？［J］.Fertil Steril，2018，109（4）：665-674.
[43]	CIRAY H N，CAMPBELL A，AGERHOLM I E，et al.Proposed guidelines on the nomenclature and annotation of dynamic human embryo monitoring by a time-lapse user group［J］.Hum Reprod，2014，29（12）：2650-2660.
[44]	ZEGERS-HOCHSCHILD F，ADAMSON G D，DYER S，et al.The international glossary on infertility and fertility care，2017［J］.Hum Reprod，2017，32（9）：1786-1801.
[45]	PARIKH F R，ATHALYE A S，NAIK N J，et al.Preimplantation genetic testing：Its evolution，where are we today？［J］.J Hum Reprod Sci，2018，11（4）：306-314.
[46]	CIMADOMO D，RIENZI L，CAPALBO A，et al.The dawn of the future：30 years from the first biopsy of a human embryo.The detailed history of an ongoing revolution［J］.Hum Reprod Update，2020，26（4）：453-473.
[47]	MADERO J I，MANOTAS M C，GARCÍA-ACERO M，et al.Preimplantation genetic testing in assisted reproduction［J］.Minerva Obstet Gynecol，2023，75（3）：260-272.
[48]	Practice Committees of the American Society for Reproductive Medicine and the Society for Assisted Reproductive Technology.The use of preimplantation genetic testing for aneuploidy （PGT-A）：a committee opinion［J］.Fertil Steril，2018，109（3）：429-436.
[49]	TUR-KASPA I，JEELANI R，DORAISWAMY P M.Preimplantation genetic diagnosis for inherited neurological disorders［J］.Nat Rev Neurol，2014，10（7）：417-424.
[50]	SCRIVEN P N.PGT-SR （reciprocal translocation） using trophectoderm sampling and next-generation sequencing：insights from a virtual trial［J］.J Assist Reprod Genet，2021，38（8）：1971-1978.
[51]	SCOTT R T，JR.，UPHAM K M，FORMAN E J，et al.Cleavage-stage biopsy significantly impairs human embryonic implantation potential while blastocyst biopsy does not：a randomized and paired clinical trial［J］.Fertil Steril，2013，100（3）：624-630.
[52]	SIMOPOULOU M，SFAKIANOUDIS K，MAZIOTIS E，et al.PGT-A：who and when？ Α systematic review and network meta-analysis of RCTs［J］.J Assist Reprod Genet，2021，38（8）：1939-1957.
[53]	YANG L，XU Y，XIA J，et al.Simultaneous detection of genomic imbalance in patients receiving preimplantation genetic testing for monogenic diseases （PGT-M）［J］.Front Genet，2022，29（13）：976131.
[54]	TIAN Y，LI M，YANG J，et al.Preimplantation genetic testing in the current era，a review［J］.Arch Gynecol Obstet，2024，309（5）：1787-1799.
[55]	LØSSL K，BENTZEN J G，PETERSEN M R，et al.Preimplantation genetic testing［J］.Ugeskr Laeger，2021，183（48）：V04210378.
[56]	LEE I T，KAPPY M，FORMAN E J，et al.Genetics in reproductive endocrinology and infertility［J］.Fertil Steril，2023，120（3 Pt 1）：521-527.
[57]	VIOTTI M.Preimplantation genetic testing for chromosomal abnormalities：Aneuploidy，mosaicism，and structural rearrangements［J］.Genes （Basel），2020，11（6）：602.
[58]	HOSNY A，PARMAR C，QUACKENBUSH J，et al.Artificial intelligence in radiology［J］.Nat Rev Cancer，2018，18（8）：500-510.
[59]	CURCHOE C L，BORMANN C L.Artificial intelligence and machine learning for human reproduction and embryology presented at ASRM and ESHRE 2018［J］.J Assist Reprod Genet，2019，36（4）：591-600.
[60]	KRAGH M F，KARSTOFT H.Embryo selection with artificial intelligence：how to evaluate and compare methods？［J］.J Assist Reprod Genet，2021，38（7）：1675-1689.
[61]	SANTOS FILHO E，NOBLE J A，POLI M，et al.A method for semi-automatic grading of human blastocyst microscope images［J］.Hum Reprod，2012，27（9）：2641-2648.
[62]	JIN L，SI K，LI Z，et al.Multiple collapses of blastocysts after full blastocyst formation is an independent risk factor for aneuploidy-a study based on AI and manual validation［J］.Reprod Biol Endocrinol，2024，22（1）：81.
[63]	MIYAGI Y，HABARA T，HIRATA R，et al.Predicting implantation by using dual AI system incorporating three-dimensional blastocyst image and conventional embryo evaluation parameters-A pilot study［J］.Reprod Med Biol，2024，23（1）：e12612.
[64]	CIMADOMO D，MARCONETTO A，TRIO S，et al.Human blastocyst spontaneous collapse is associated with worse morphological quality and higher degeneration and aneuploidy rates：a comprehensive analysis standardized through artificial intelligence［J］.Hum Reprod，2022，37（10）：2291-2306.
[65]	DIAKIW S M，HALL J M M，VERMILYEA M D，et al.Development of an artificial intelligence model for predicting the likelihood of human embryo euploidy based on blastocyst images from multiple imaging systems during IVF［J］.Hum Reprod，2022，37（8）：1746-1759.
[66]	KHOSRAVI P，KAZEMI E，ZHAN Q，et al.Deep learning enables robust assessment and selection of human blastocysts after in vitro fertilization［J］.NPJ Digit Med，2019，4（2）：21.
[67]	SUN L，LI J，ZENG S，et al.Artificial intelligence system for outcome evaluations of human in vitro fertilization-derived embryos［J］.Chin Med J （Engl），2024，137（16）：1939-1949.
[68]	BORMANN C L，THIRUMALARAJU P，KANAKASABAPATHY M K，et al.Consistency and objectivity of automated embryo assessments using deep neural networks［J］.Fertil Steril，2020，113（4）：781-787.e781.
[69]	GLATSTEIN I，CHAVEZ-BADIOLA A，CURCHOE C L.New frontiers in embryo selection［J］.J Assist Reprod Genet，2023，40（2）：223-234.

Research Progress and Frontier Exploration of Quality Evaluation Indicators for Domestic Animal Blastocysts

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