山羊脐带间充质干细胞分离培养与治疗小鼠药物性肝损伤

doi:10.11843/j.issn.0366-6964.2025.08.042

Abstract

Abstract:

The aim of this study was to observe the isolation and culture of Leizhou goat umbilical cord mesenchymal stem cells (LZG-UCMSCs) and the effect on the treatment of drug-induced liver injury (DILI) in mice. Umbilical cord mesenchymal stem cells were isolated and cultured, growth curve, cell cloning, RT-PCR, immunofluorescence and induction experiments were performed from nine-year-old Leizhou goat placenta. 36 ICR mice were randomly divided into 3 groups: blank control group, model injury group and stem cell treatment group, with 12 mice in each group. The rats in the model group and the treatment group were given gavage at a dose of 15 mg·kg^-1 for 4 weeks. Aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were measured. The results showed that the cell morphology of the isolated and cultured LZG-UCMSCs was normal, with the characteristics of UCMSCs. Compared with the control group, the activities of ALT, AST and ALP in the injured group were significantly increased (P < 0.05). Compared with injury group, ALT, AST and ALP in treatment group were significantly decreased (P < 0.01). The results suggest that normal UCMSCs can be isolated from the placenta of Lezhou goat, and LZG-UCMSCs has a certain therapeutic effect on DILI in mice, thus providing reference value for further treatment of homologous goat DILI by LZG-UCMSCs.

Key words: goat, umbilical cord, mesenchymal stem cells, mouse, liver injury, treatment

CLC Number:

S856.4

FENG Jintao, YAN Yan, ZONG Junlin, HU Yuqing, LI Xiaorui, GUAN Weijun, LI Yaokun. Isolation and Culture of Goat Umbilical Cord Mesenchymal Stem Cells for the Treatment of Drug-induced Liver Injury in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(8): 4053-4064.

Figures/Tables 11

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基因 Gene	引物序列(5′→3′) Sequence	片段长度/bp Product length
GAPDH	F：GGCGTGAACCACGAGAAGTA	141
	R：GGCGTGGACAGTGGTCATAA
CD29	F：GAATCGGGTTTGGCTCTTTCGT	309
	R：AATGGGCTCGTGCAGTTCTGTT
CD45	F：GATCCACCCCCAACCTCATCT	196
	R：AACTGCAAGAATCAAAGCCA
CD73	F：TGGGAGGAATTCACGTGGTG	104
	R：AGGCTCATAACTGGGCACAC
CD90	F：CTGACCCGTGATACAAAGAAGTGG	216
	R：CCTTGTTGGAGACGGTGGGATT
CD105	F：TGCACCATCATAAGCCTGACCTT	136
	R：TGACCACCTCGTTACTGACCACAT

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Isolation and Culture of Goat Umbilical Cord Mesenchymal Stem Cells for the Treatment of Drug-induced Liver Injury in Mice

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