Table 1

Gene and primer sequence"

Fig. 1

Morphological observation of cADMSCs and detection of EXO A. Optical microscopy imaging of isolating culture cADMSCs (Scale, 200 μm in the left Fig, and 100 μm in the right Fig); B. CCK-8 detection of the effect of different concentrations of CUR on the proliferation of cADMSCs; C. Detection of excitation luminescence in cell supernatant before and after centrifugation (425 nm Emission light: Changes in fluorescence intensity at 530 nm); D. Detection of PBS and centrifugation of PBS with added CUR, followed by excitation luminescence at 425 nm; Emission light: Changes in fluorescence intensity at 530 nm; E. Transmission electron microscopy imaging of EXO and EXO-CUR (Scale bar=100 nm); F. Western blot identification of exosome markers Alix, CD63, and TSG101. *. P < 0.05, **. P < 0.01, ***. P < 0.001, ns indicating no statistical significance"

Fig. 2

Statistics on wound healing and wound area in each group of mice A. Wound photos of each group of mice on days 1, 3, 5, 7, 9, and 11 after wound modeling; B. Statistics of the wound area in each group of mice. *. P < 0.05"

Fig. 3

Detection of histopathological changes in skin injury sites and gene expression related to skin injury healing in each group of mice A. HE staining of newborn skin in each group of mice (Scale bar=100 μm); B. Masson staining of newborn skin in each group of mice (Scale bar=100 μm); C. The proportion of collagen fibers in the newborn skin of each group of mice; D. Immunohistochemical staining (VEGFA) of newborn skinin each group of mice (Scale bar=100 μm); E. RT-qPCR detection of relative expression levels of scar related genes (SOX9, TGF-β1, TGF-β3, Twist1), anti-inflammatory factor IL-10, and skin healing related genes (VEGFA, FGF2) in newborn skin tissues; F. RT qPCR detection of relative expression levels of ECM synthesis related genes (COL1A1, COL3A1, MMP2 and LAMA4) in newborn skin tissues. *. P < 0.05, **. P < 0.01, ***. P < 0.001"

Fig. 4

ELISA detection of the concentrations of oxidative stress, inflammatory molecules, and angiogenic related molecules in the tissues and serum of mice in each group A. The concentration of VEGF in the skin tissue of each group of mice; B. The concentration of FGF2 in the skin tissue of each group of mice; C. The concentration of EGF in the skin tissue of each group of mice; D. The concentration of VEGF in the serum of each group of mice; E. The concentration of FGF2 in the serum of each group of mice; F. The concentration of ROS in the serum of each group of mice; G. The concentration of GSH in the serum of each group of mice; H. The concentration of IL-1β in the serum of each group of mice; I. The concentration of TNF-α in the serum of each group of mice. Statistical significance was as follows: *. P < 0.05, **. P < 0.01, ***. P < 0.001"

Fig. 5

Statistics on wound healing and wound area in each group of canines A. Wound photos of each group of canines on days 1, 3, 5, 7, 9, 11 and 13 after wound modeling; B. Statistics of wound area in each group of canines; C. RT-qPCR detection of skin healing related genes (VEGFA, FGF2 and Wnt4) in newborn skin; D. HE staining of newborn skin of canine in each group (Scale bar=100 μm); E. Masson staining of newborn skin of canine in each group (Scale bar=100 μm). *. P < 0.05, **. P < 0.01, ***. P < 0.001"