Table 1

shRNA primer sequences"

Table 2

Primer sequences for RT-qPCR"

Fig. 1

Results of construction of the pLKO.1-shIRFs recombinant vector A. Electrophoresis results of double digestion products of pLKO.1 vector (Marker. Molecular weight standard of DNA marker DL15000); B. Electrophoresis results of PCR amplification products of bacterial broth after transformation with pLKO.1-shIRFs recombinant vector (Marker. Molecular weight standard of DNA marker DL2000); C. pLKO.1-shIRFs recombinant vector after transformation of bacterial liquid sequencing results, the dark region is shIRFs sequence"

Fig. 2

Validation of knockdown efficiency in PK15 shIRFs knockdown cell lines A. RT-qPCR analysis of IRFs gene transcript levels in knockdown cell lines, *.P < 0.05, **.P < 0.001, ***.P < 0.000 1; B. Western blot analysis of IRF-3 protein expression levels in PK15 shIRF-3 cells; C. Immunofluorescence assay was performed to detect the expression level of IRF-3 protein in PK15 shIRF-3 cells"

Fig. 3

The effect of IRFs gene knockdown on PRV replication in PK15 cells was verified A. Fluorescence imaging to observe the fluorescence intensity in PK15 control and PK15 shIRFs cells after infection with PRV-GFP.B. Flow cytometry to detect the proportion of cells with fluorescence in PK15 control and PK15 shIRFs cells after infection with PRV-GFP.C. Plaque assay to determine the titer of progeny virus in PK15 control and PK15 shIRFs cells after infection with PRV-QXX.D. RT-qPCR to measure the transcription level of the PRV-gB gene in PK15 control and PK15 shIRFs cells after infection with PRV-QXX.E. Western blot to assess the protein expression level of PRV-gB in PK15 control and PK15 shIRFs cells after infection with PRV-QXX. *.P < 0.05, **.P < 0.001, ***.P < 0.000 1"

Fig. 4

The effect of IRFs knockdown on the transcription level of inflammatory factors was verified A. RT-qPCR was used to detect the transcriptional levels of IFN-β in the presence of PRV-QXX infection after IRFs knockdown; B. RT-qPCR was used to detect ISG-15 transcript levels in the presence of PRV-QXX infection after IRFs knockdown; C. RT-qPCR was used to detect the transcriptional levels of IL-6 in the presence of PRV-QXX infection after IRFs knockdown. *.P < 0.05, **.P < 0.001, ***.P < 0.000 1, ns. P>0.05"