Abstract:

In this study, the effect of a patent-protected strain, Lactobacillus salivarius XP132, on the gut microbiota of healthy white feathered broiler breeders was evaluated using 16S rDNA sequencing. Twelve 6-month-old white-feathered broilor breeders were selected and randomly divided into control growp and experimeat group, with 6 broilers in each group. Lactobacillus salivarius XP132 fermentation broth with 1×10⁹ CFU was fed to broiler breeders in experiment group per day for 4 weeks, while broiler breeders in the control group were fed with PBS. Gut microbiota were determined by 16S rDNA sequencing. The results showed that after feeding Lactobacillus salivarius XP132 the species richness was higher in the experimental group than that in the control group using α diversity analysis. At the genus level of microbial composition, there was an increase in the abundance of beneficial species such as Laehnospiraceae NK4A136 spp., Clostridium spp., Paramycetes spp., and Rhodococcus spp. in the gut microbiota of the experimental group compared to the control group. While at the species level, there was a significant increase in the abundance of beneficial species such as Lactobacillus spp., Anaplasmatales spp., and Lactobacillus rhamnosus spp. in the gut microbiota of the experimental group. The results showed that feeding Lactobacillus salivarius XP132 changed the richness of intestinal microbiota and increased the species abundance of beneficial bacteria in white-feathered broilers.

Key words: gut microbiota, Lactobacillus salivarius, 16S rDNA, white-feathered broiler breeder