宿主膜联蛋白A2与伪狂犬病病毒US3蛋白互作及其对细胞凋亡的影响

doi:10.11843/j.issn.0366-6964.2022.11.018

Abstract

Abstract: US3 protein kinase is a critical virulence factor of pseudorabies virus (PRV). It is involved in many biological process, including cytoskeletal rearrangement, viral replication and transmission, antagonist apoptosis and interferon induced anti-viral response. The identification of US3 substrates is directly connected to understanding US3 functions and mechanisms. Here, we confirmed a host protein annexin A2 (ANXA2) can interact with US3 by co-immunoprecipitation and pull-down assay. Knock down the expression of ANXA2 can significantly affect the PRV proliferation on PK-15 cells, which was verified through quantitative PCR, viral titer and immunoblot analysis. Furthermore, we found that ANXA2 could inhibit apoptosis of PK-15 cells indued by PRV infection and apoptotic stimulator, suggesting that ANXA2 negatively regulates apoptosis. This study expands the host protein members that interact with US3 and deepens our understanding of the biological functions of ANXA2.

Key words: pseudorabies virus, US3, annexin A2, protein interaction, apoptosis

CLC Number:

S852.659.1

GUO Zhenhua, LI Xiang, WENG Maoyang, JIN Qianyue, GUO Junqing, XING Guangxu, ZHANG Gaiping. Host Annexin A2 Interacts with US3 of PRV and Its Effects on Apoptosis[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 3927-3935.

References 20

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Host Annexin A2 Interacts with US3 of PRV and Its Effects on Apoptosis

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