1株伪狂犬病病毒的基因变异及其对小鼠的致病性分析

doi:10.11843/j.issn.0366-6964.2023.04.039

Abstract

Abstract: This study aimed to understand the molecular genetic variation and virulence characteristics of porcine pseudorabies virus (PRV) in China. The main glycoprotein gene sequences (gB, gC and gE) of PRV ZJ strain isolated from a pig farm inoculated with Bartha-K61 vaccine were determined. and its pathogenicity to 6-week-old mice was studied. Phylogenetic analysis results showed that this strain was highly homologous to epidemic strains in China after 2012, and had the genetic characteristics of the mutant strain. However, there was a unique change of S⁵¹⁰G in the amino acid position 510 of gE gene, which was different from the mutant strain. ZJ strain was highly pathogenic to BALB/c mice, and the challenged mice showed typical neurological symptoms. The mortality rate reached 100% on day 5 after the mice were infected with 100 LD₅₀. This study laid a foundation for further exploration of the variation characteristics and pathogenesis of this virus.

Key words: pseudorabies virus, variantstrain, genetic evolution analysis, pathogenicity

CLC Number:

S852.659.1

NI Zheng, YE Weicheng, CHEN Liu, YUN Tao, HUA Jionggang, ZHU Yinchu, ZHANG Cun. Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1766-1770.

References 15

[1]	FREULING C M, MVLLER T F, METTENLEITER T C. Vaccines against pseudorabies virus (PrV)[J]. Vet Microbiol, 2017, 206:3-9.
[2]	RZIHA H J, METTENLEITER T C, OHLINGER V, et al. Herpesvirus (pseudorabies virus) latency in swine:occurrence and physical state of viral DNA in neural tissues[J]. Virology, 1986, 155(2):600-613.
[3]	VERPOEST S, CAY B, FAVOREEL H, et al. Age-dependent differences in pseudorabies virus neuropathogenesis and associated cytokine expression[J]. J Virol, 2017, 91(2):e02058-16.
[4]	LIU Q Y, WANG X J, XIE C H, et al. A novel human acute encephalitis caused by pseudorabies virus variant strain[J]. Clin Infect Dis, 2021, 73(11):e3690-e3700.
[5]	POMERANS L E, REYNOLDS A E, HENGARTNER C J. Molecular biology of pseudorabies virus:impact on neurovirology and veterinary medicine[J]. Microbiol Mol Biol Rev, 2005, 69(3):462-500.
[6]	SZPARA M L, TAFURI Y R, PARSONS L, et al. A wide extent of inter-strain diversity in virulent and vaccine strains of alphaherpesviruses[J]. PLoS Pathog, 2011, 7(10):e1002282.
[7]	BRITTLE E E, REYNOLDS A E, ENQUIST L W. Two modes of pseudorabies virus neuroinvasion and lethality in mice[J]. J Virol, 2004, 78(23):12951-12963.
[8]	CURANOVIC D, ENQUIST L W. Virion-incorporated glycoprotein B mediates transneuronal spread of pseudorabies virus[J]. J Virol, 2009, 83(16):7796-7804.
[9]	XIA L M, SUN Q Y, WANG J J, et al. Epidemiology of pseudorabies in intensive pig farms in Shanghai, China:herd-level prevalence and risk factors[J]. Prev Vet Med, 2018, 159:51-56.
[10]	LIU C, LIU Y H, TIAN Y, et al. Genetic characterization and mutation analysis of Qihe547 Aujeszky's disease virus in China[J]. BMC Vet Res, 2018, 14(1):218.
[11]	KLUPP B G, HENGARTNER C J, METTENLEITER T C, et al. Complete, annotated sequence of the Pseudorabies virus genome[J]. J Virol, 2004, 78(1):424-440.
[12]	HU D F, ZHANG Z D, LV L, et al. Outbreak of variant pseudorabies virus in Bartha-K61-vaccinated piglets in central Shandong Province, China[J]. J Vet Diagn Invest, 2015, 27(5):600-605.
[13]	TONG W, LIU F, ZHENG H, et al. Emergence of a Pseudorabies virus variant with increased virulence to piglets[J]. Vet Microbiol, 2015, 181(3-4):236-240.
[14]	FAN J D, ZENG X D, ZHANG G Q, et al. Molecular characterization and phylogenetic analysis of pseudorabies virus variants isolated from Guangdong province of southern China during 2013-2014[J]. J Vet Sci, 2016, 17(3):369-375.
[15]	XU R S, WEI S, ZHOU G B, et al. Multiplex TaqMan locked nucleic acid real-time PCR for the differential identification of various meat and meat products[J]. Meat Sci, 2018, 137:41-46.

Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice

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