Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (10): 4346-4359.doi: 10.11843/j.issn.0366-6964.2024.10.010

• Animal Genetics and Breeding • Previous Articles     Next Articles

Genome-Wide Association Study of Wool Economic Traits in Aohan Fine Wool Sheep

Xiaokun LIN(), Mengmeng DU, Lisheng ZHOU, Zhengang HUANG, Di WANG, Donghui ZHOU, Xinxin CAO, Jianning HE, Jinshan ZHAO*(), Hegang LI*()   

  1. College of Animal Science and Technology, Qingdao Agricultural University, Qingdao 266109, China
  • Received:2024-04-15 Online:2024-10-23 Published:2024-11-04
  • Contact: Jinshan ZHAO, Hegang LI E-mail:243252756@qq.com;201501005@qau.edu.cn;201701018@qau.edu.cn

Abstract:

The study aimed to identify new molecular markers and candidate genes for wool traits in Aohan fine wool sheep using genome-wide association study. The ear tissue and wool of 1-2 years old healthy Aohan fine wool sheep were collected as experimental materials, 248 ewes and 81 rams, totaling 329. The wool traits were detected (including fiber diameter, natural length, elongation length, and elongation ratio), and the phenotypic data were subjected to descriptive statistics and correlation analysis. All individuals were genotyped using the 40K liquid phase SNP chip for sheep. The chip data were quality controlled using PLINK 1.07 software, and the quality-controlled data were analyzed for population structure using GCTA software and PopLDdecay software. The GMEMA mixed linear model was used to perform a genome-wide association study (GWAS) on 4 wool traits, and online software was used to conduct GO and KEGG enrichment analyses on candidate genes. After quality control, 329 individuals with 30 079 SNP loci were used for subsequent analysis. Through GWAS analysis, 4 SNP loci were identified as significantly associated with wool economic traits across the genome, located on chromosomes 1, 6, and 8. Additionally, 9 SNP loci were identified as significantly associated with wool traits at the chromosome level, located on chromosomes 3, 5, 8, 11, 18, 21, 22, and 25, and 39 candidate genes were identified as potentially affecting wool traits. The results of this study provide important references for further exploring the genetic mechanisms and molecular breeding markers of wool traits in Baohan fine wool sheep.

Key words: Aohan fine wool sheep, wool traits, genome-wide association study, candidate gene

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