戴瑞奶绵羊产奶性状的全基因组关联分析

doi:10.11843/j.issn.0366-6964.2021.011.030

Abstract

Abstract: The study aimed to identify and map the genetic markers and functional genes which associated with milk production traits through genome-wide association analysis (GWAS) of milk production traits in Dairy Meade(DM) sheep. In this study, 135 DM sheep were collected and the whole genome resequencing technology was used. The single nucleotide polymorphisms (SNPs) were identified through SAMTOOLS software. The quality control (QC) was performed by Plink v1.90 software. The genome-wide association analysis of milk production traits in DM sheep was performed based on the QC results using the mixed linear model of GEMMA v 0.98.1. The results showed that there was one SNP which was significantly correlated and 8 SNPs were potentially significantly correlated with D90 average daily milk yield trait at genome-wide; The associated candidate genes included TRNAQ-CUG-2, LOC114117240, ACADL, MYL1, CHD6 and SLCO3A1. There were 2 SNPs which were potentially significantly correlated with D150 average daily milk yield trait; The associated candidate genes included PRMT6 and RNF180. There were 2 SNPs which were potentially significantly correlated with lactation period trait; The associated candidate genes included PRMT6, TRNAW-CCA-68 and TRNAS-GGA-61. Further gene functional analysis suggested that ACADL and SLCO3A1 were likely candidate genes which affect milk production traits of DM sheep. This study provides a certain basis for the exploration of milk production traits molecular mechanism in dairy sheep. The result could provide a certain theoretical reference for new dairy sheep breeding in China.

Key words: genome-wide association analysis, Dairy Meade(DM) sheep, milk production traits, resequencing, candidate gene

CLC Number:

S826.2

MI Bunong, ZHANG Liguo, BAI Urhan, GUO Yulin, WANG Chunwei, XU Quanzhong, FENG Shuang, LI Guangpeng, SU Xiaohu, ZHANG Li. Genome-wide Association Study of Milk Production Traits in Dairy Meade Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3294-3303.

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Genome-wide Association Study of Milk Production Traits in Dairy Meade Sheep

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