立华麻黄鸡体重和肉品质性状全基因组关联分析

doi:10.11843/j.issn.0366-6964.2023.12.010

Abstract

Abstract: The purpose of this study was to explore the genetic loci and functional genes affecting the weight and meat quality traits of broilers by genome-wide association study (GWAS), and to accumulate theoretical basis for breeding of fast yellow-feathered broilers. The test subjects were 393 Lihua ephedra terminal paternal roosters, which were weighed and collected breast muscle to determine meat quality traits at 60 days of age. Individual genetic variants were obtained by whole genome resequencing, and GWAS analysis of body weight and meat quality traits was performed using PLINK, GEMMA and R software. A total of 2 SNP loci significantly associated with body weight and 8 SNP loci significantly associated with meat quality traits were screened (P<1.15×10^-7); The 52 loci associated with body weight and 296 loci associated with meat quality were identified at the potentially significant level (P<2.31×10^-6). By annotating these associated SNPs, a total of 99 candidate genes related to body weight, 27 to shear force, 28 to water binding capacity, 57 to pH, and 113 to meat color were selected. It was concluded that FSTL3, MBNL3, NPFF, PFKM, PRKG1, SLC13A5, MDFIC, FGD3, AQP1 may be key candidate genes for body weight and meat quality traits in broilers. The above research results provide important genetic variation sites and subsequent genes for important economic traits of fast type yellow-feathered broilers, and provide key basic data for improving molecular breeding methods for high-quality broilers and accelerating the progress of breed selection.

Key words: fast-growing yellow-feathered broilers, genome-wide association study (GWAS), body weight, meat quality

CLC Number:

S831.2

FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.

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Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens

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