75株蛋鸡源沙门菌的MLST分型与耐药性分析

doi:10.11843/j.issn.0366-6964.2022.05.030

Abstract

Abstract: The aim of this study was to investigate the serotypes, drug sensitivity and virulence genes of Salmonella isolated from laying hens in Yunnan between July 2017 and May 2019. A total of 75 Salmonella strains were isolated from the liver tissues of sick chickens. MLST serotyping, drug sensitivity, related resistance genes and virulence genes were detected. A total of 54 strains (72.00%) and 21 strains (28.00%) were determined to be ST78 and ST92, respectively, by MLST. The resistance rate to penicillin was 100%, and the resistance rates were 26.67% to tetracycline, 26.67% to doxycycline, 22.67% to sulfamethoxazole, 18.67% to amoxicillin, 16.00% to ampicillin, 14.67% to enrofloxacin, 8.00% to streptomycin, 2.67% to ciprofloxacin, and 1.33% to gentamicin. There were seven profiles of drug resistance spectrum, with 28.00% of the strains (mainly S. Gallinarum) showing multiple resistance. The detection rates of resistance genes tetA, sul2 and blaTEM were 26.67%, 10.67% and 8.00%, respectively. The virulence genes mogA, mgtC, bcfA, araB, stn and spvC were detected in all 75 strains, while the detection rate of spvB was 89.33%. The present study showed that S. Gallinarum and S. Pullorum were the main epidemic serotypes of Salmonella in laying hens in Yunnan, and that the multiple drug resistance was predominant due to the high prevalence of drug resistance genes and virulence genes harbored in the Salmonella strains.

Key words: Salmonella isolated from laying hens, MLST, drug resistance, virulence gene, Yunnan

CLC Number:

WANG Xi, LI Ke, LI Tingcui, YAN Hongya, ZHAO Rong, CHANG Zhishun, LIAO Ming, SUN Minhua, XIN Aiguo. MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1626-1631.

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MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens

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