广东省羊源肺炎克雷伯菌遗传进化与毒力基因及耐药性分析

doi:10.11843/j.issn.0366-6964.2023.01.030

Abstract

Abstract: This study investigated the virulence genes, drug resistance genes, drug resistance spectrum and genetic diversity of Klebsiella pneumoniae from sheep in Guangdong province. One hundred and fifty sheep nose swab samples were collected from sheep farms with different scales in 3 cities of Guangdong province. The pathogenic bacteria were isolated and identified by means of culture, purification, staining and microscopic examination. The biological characteristics of the isolated strains, such as virulence genes and drug resistance etc., were studied by means of molecular biology and drug sensitivity test according to CLSI manual. The results showed that 42 strains of K. pneumoniae were isolated from the samples. The drug susceptibility test results showed that the drug resistance rate of amoxicillin and ofloxacin was up to 100%, and there were different degrees of resistance to other drugs; the virulence gene test results showed that rmpA, kfuBC and ureA were 88.0%, 85.7% and 83.3%, respectively. The detection results of drug resistance genes showed that the detection rates of drug resistance genes aphA, qnrA, aacC4, aacC2 were 42.9%, 40.5%, 28.6%, 23.8%, and the detection rates of other drug resistance genes were low. Eric-pcr typing results showed that the homology was more than 80.0%, which could be divided into seven groups, among which population Ⅰ was the dominant population, accounting for 66.6%.This study was conducted to analyze the virulence genes, epidemic status and drug resistance of sheep K. pneumoniae in Guangdong area, which has certain guiding significance for the prevention and control of sheep K. pneumoniae.

Key words: Klebsiella pneumoniae, drug susceptibility test, virulence gene, drug resistance

CLC Number:

S852.61

ZHANG Kaichuan, WANG Jinyu, LI Shoujun, JIA Kun. Isolation, Identification and Biological Characteristics of Klebsiella pneumoniae from Sheep in Guangdong Province[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 328-337.

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Isolation, Identification and Biological Characteristics of Klebsiella pneumoniae from Sheep in Guangdong Province

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