鼠伤寒沙门菌BaeSR对氟喹诺酮类药物耐药性的调控机制

doi:10.11843/j.issn.0366-6964.2022.03.022

Abstract

Abstract: BaeSR is an important two component system in Salmonella Typhimurium, which affects bacterial resistance by regulating the transcription and expression of downstream target genes.The aim of this study was to explore the regulatory mechanism of BaeR overexpression in S. Typhimurium to fluoroquinolones (FQs), and to find the potential target gene of BaeR. In this study, we first expressed, purified and validated the BaeR protein, and screened the drug-related differentially expressed genes (include ompW, sodB, tolC, mdtD, spy and marR) from transcriptomics as potential target genes of BaeR. Combined with LacZ reporter gene fusion assay and electrophoretic mobility shift assay (EMSA), we studied the resistance mechanism of BaeSR to S. Typhimurium. The target band appeared at 28 ku after purification, which indicated that BaeR protein was successfully expressed. LacZ reporter gene fusion assay results show that, BaeR can positively regulate the expression of ompW, sodB, tolC, mdtD, spy and marR after overexpression. EMSA results indicate that BaeR protein can directly bind to the promoter region of marR and regulate the expression of marR gene. In conclusion, BaeSR can positively regulate the transcriptional expression of target genes ompW, sodB, tolC, mdtD, spy and marR in S. Typhimurium, and directly regulate the expression of marR, thus affecting the resistance of S. Typhimurium to FQs.

Key words: Salmonella Typhimurium, BaeSR, drug resistance, EMSA

CLC Number:

S852.61

ZHANG Lin, LU Fang, FU Hengfeng, JIANG Xidi, WEI Qiling, QI Caili, GAO Haixia, LI Lin. Regulation Mechanism of BaeSR on Fluoroquinolones Resistance in Salmonella Typhimurium[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 894-903.

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Regulation Mechanism of BaeSR on Fluoroquinolones Resistance in Salmonella Typhimurium

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