1株猪源ST-35型肺炎克雷伯菌的致病性和药物敏感性分析

doi:10.11843/j.issn.0366-6964.2022.12.021

Abstract

Abstract: This study was conducted to understand the pathogenicity and drug sensitivity of Klebsiella pneumoniae (Kp) isolated from pig lung tissue. In this study, the isolate was identified based on morphology observation, PCR detection, 16S rRNA sequencing and MLST multi-locus sequence analysis. The pathogenicity of the isolate was determined by virulence gene detection and mouse challenge test, and the drug sensitivity was analyzed by antibiotics and traditional Chinese medicine. The results showed that the isolate was Gram-negative bacilli, and the biochemical results were consistent with the biochemical characteristics of Kp. 16S rRNA gene sequence alignment analysis and phylogenetic tree construction identified the isolated strain as Kp, named KP-0728. MLST analysis showed that KP-0728 belonged to ST-35 type and converged with ST-875. KP-0728 contained four virulence genes, uge, wabG, fimH and kfu. When KP-0728 was infected with mice intraperitoneally, different degrees of pathological changes in various organs of the infected mice were induced, and high dose infection of KP-0728 can cause death in mice. KP-0728 carried four resistance genes bla-SHV, sul2, tetA and aadA1, and was resistant to ampicillin, imipenem, gentamicin, tetracycline and cotrimoxazole and sensitive to ceftriaxone, cefalexin, cefmetazole, azithromycin, ofloxacin, polymyxin B and teicoplanin. The results of in vitro antibacterial experiments of nine Chinese medicines showed that Poria cocos had the most significant antibacterial effect on KP-0728. The data of animal treatment test also showed that Poria cocos had inhibitory effect on KP-0728 in vivo. In conclusion, a porcine Kp type ST-35 was isolated in this study, carrying a variety of virulence genes and drug resistance genes. Artificial infection of Kp in mice can lead to pathological changes and the median lethal dose (LD₅₀) was 4.56×10⁶ CFU, and KP-0728 is sensitive to traditional Chinese medicine Poria cocos and many kinds of antibiotics. The results provide a reference for the prevention and treatment of Kp and vaccine research.

Key words: Klebsiella pneumoniae, isolation and identification, pathogenicity, drug resistance, traditional Chinese medicine

CLC Number:

S852.61

LI Huaming, XIANG Wei, LU Wenbing, LIU Feng, LEI Liancheng, ZHANG Fuxian. Pathogenicity and Drug Sensitivity Analysis of a Porcine Klebsiella pneumoniae Type ST-35[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4356-4366.

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Pathogenicity and Drug Sensitivity Analysis of a Porcine Klebsiella pneumoniae Type ST-35

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