新疆部分地区牛羊源产志贺毒素大肠埃希菌菌株检测与分析

doi:10.11843/j.issn.0366-6964.2020.10.020

Abstract

Abstract: Shiga toxin-producing Escherichia coli(STEC) is a new class of highly pathogenic food-borne pathogens carrying a prephage encoding one or two Shiga toxin genes. It has become an important public health issue that threatens human health. The present work aimed to characterize STEC strains isolated from cattle and sheep at various stages, in parts of Xinjiang, in terms of the presence of prevalence, genetic diversity, and antimicrobial susceptibility to 17 common antibiotics. Through amplification of four virulence genes (stx1, stx2, eae, hlyA)by PCR and ERIC-PCR genotyping to detection STEC isolates. In the present study, a total of 64 STEC strains were isolated from 431 samples from slaughterhouses, farms and markets. Of these, 31 (48.4%) of the isolates harbored stx1 + stx2, and only 29 (45.3%) of the isolates possessed stx1, only 4 (6.3%) of the isolates harbored stx2, and 1 isolates harbored all the 4 virulence genes. Drug sensitivity tests found that STEC strains displayed 7 antimicrobial resistance to midecamycin(61%), cephalothin(4.7%), cefoxitin(4.7%), ampicillin(3.1%), piperacillin(1.6%), tobramycin(1.6%), cefazolin(1.6%). The ERIC-PCR results showed a polymorphic distribution, which was divided into two clusters of A (36 strains) and B (28 strains). STEC strains isolated from cattle and sheep at various stages, in parts of Xinjiang, some of which might have the potential to cause food contamination and human diseases.

Key words: Shiga toxin-producing Escherichia coli, virulence gene, drug resistance, ERIC-PCR

CLC Number:

S852.61

ZHENG Xiaofeng, ZHANG Yan, LIU Yingyu, ZHU Mingyue, ZHENG Xiaoqin, LU Wei, JIANG Jindou, ZHU Menghan. Detection and Analysis of Shiga Toxin-producing Escherichia coli Isolates from Cattle and Sheep Sources in Some Regions of Xinjiang, China[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2518-2527.

References 34

[1]	YANG X, BAI X N, ZHANG J, et al. Escherichia coli strains producing a novel Shiga toxin 2 subtype circulate in China[J]. Int J Med Microbiol, 2020, 310(1):151377.
[2]	GRIFFIN P M, TAUXE R V. The epidemiology of infections caused by Escherichia coli O157:H7, other enterohemorrhagic E. coli, and the associated hemolytic uremic syndrome[J]. Epidemiol Rev, 1991, 13(1):60-98.
[3]	FAN R Y, SHAO K, YANG X, et al. High prevalence of non-O157 Shiga toxin-producing Escherichia coli in beef cattle detected by combining four selective agars[J]. BMC Microbiol, 2019, 19(1):213.
[4]	KING L A, MAILLES A, MARIANI-KURKDJIAN P, et al. Community-wide outbreak of Escherichia coli O157:H7 associated with consumption of frozen beef burgers[J]. Epidemiol Infect, 2009, 137(6):889-896.
[5]	巴鹏斌, 孟琼, 白向宁, 等. 非O157产志贺毒素大肠埃希菌研究进展[J]. 中国人兽共患病学报, 2017, 33(2):156-162.BA P B, MENG Q, BAI X N, et al. Research progress on non-O157 Shiga toxin-producing Escherichia coli[J]. Chinese Journal of Zoonoses, 2017, 33(2):156-162. (in Chinese)
[6]	白向宁, 赵爱兰, 夏胜利, 等. 非O157产志贺毒素大肠杆菌分离株的多位点序列分型研究[J]. 中国人兽共患病学报, 2012, 28(6):544-548, 560.BAI X N, ZHAO A L, XIA S L, et al. Multilocus sequence typing of non-O157 Shiga toxin-producing Escherichia coli isolates[J]. Chinese Journal of Zoonoses, 2012, 28(6):544-548, 560. (in Chinese)
[7]	PENG Z, LIANG W, HU Z Z, et al. O-serogroups, virulence genes, antimicrobial susceptibility, and MLST genotypes of Shiga toxin-producing Escherichia coli from swine and cattle in Central China[J]. BMC Vet Res, 2019, 15(1):427.
[8]	FRANK C, WERBER D, CRAMER J P, et al. Epidemic profile of Shiga-toxin-producing Escherichia coli O104:H4 outbreak in Germany[J]. N Engl J Med, 2011, 365(19):1771-1780.
[9]	白向宁, 王红, 赵爱兰, 等. 食源性产志贺毒素大肠杆菌的分离及菌株特征分析[J]. 中国食品卫生杂志, 2014, 26(4):312-317.BAI X N, WANG H, ZHAO A L, et al. Isolation and characterization of Shiga toxin-producing Escherichia coli isolates in foods[J]. Chinese Journal of Food Hygiene, 2014, 26(4):312-317. (in Chinese)
[10]	BOONE J T, CAMPBELL D E, DANDRO A S, et al. A rapid immunoassay for detection of Shiga toxin-producing Escherichia coli directly from human fecal samples and its performance in detection of toxin subtypes[J]. J Clin Microbiol, 2016, 54(12):3056-3063.
[11]	MENG Q, BAI X N, ZHAO A L, et al. Characterization of Shiga toxin-producing scherichia coli isolated from healthy pigs in China[J]. BMC Microbiol, 2014, 14(1):5.
[12]	KARAMA M, MAINGA A O, CENCI-GOGA B T, et al. Molecular profiling and antimicrobial resistance of Shiga toxin-producing Escherichia coli O26, O45, O103, O121, O145 and O157 isolates from cattle on cow-calf operations in South Africa[J]. Sci Rep, 2019, 9(1):11930.
[13]	GALARCE N, ESCOBAR B, SÁNCHEZ F, et al. Virulence genes, Shiga toxin subtypes, serogroups, and clonal relationship of Shiga toxin-producing Escherichia coli strains isolated from livestock and companion animals[J]. Animals, 2019, 9(10):733.
[14]	BAI J F, SHI X R, NAGARAJA T G. A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157:H7[J]. J Microbiol Methods, 2010, 82(1):85-89.
[15]	曹芸. 检测副猪嗜血杆菌的乳胶凝集试验及ERIC-PCR方法的建立与应用[D]. 武汉:华中农业大学, 2009.CAO Y. Development and application of a simple latex agglutination test and ERIC-PCR method for detection of Haemophilus parasuis[D]. Wuhan:Huazhong Agricultural University, 2009. (in Chinese)
[16]	中华人民共和国卫生部, 中国国家标准化管理委员会. GB/T 4789. 3-2008食品卫生微生物学检验大肠菌群计数[S]. 北京:中国标准出版社, 2009:1-8.Ministry of Health of the PRC, Standardization Administration of China. GB/T 4789. 3-2008 Microbiological examination of food hygiene-enumeration of coliforms[S]. Beijing:China Standard Press, 2009:1-8. (in Chinese)
[17]	赫然, 张颖, 王强, 等. 利用微波炉和煮沸法快速制备大肠杆菌基因组DNA PCR模板[J]. 中山大学学报:自然科学版, 2004, 43(S1):80-81.HE R, ZHANG Y, WANG Q, et al. Rapid preparation of PCR templates of genomic DNA from Escherichia coli by using microwave and boiling[J]. Acta Scientiarum Naturalium Universitatis Sunyatseni, 2004, 43(S1):80-81. (in Chinese)
[18]	THONG K L, LAI K S, GANESWRIE R, et al. Pulsed-field gel electrophoresis of multidrug-resistant and -sensitive strains of Pseudomonas aeruginosa from a Malaysian hospital[J]. Jan J Infect Dis, 2004, 57(5):206-209.
[19]	孙晖, 白向宁, 赵爱兰, 等. 牦牛携带的产志贺毒素大肠杆菌分离株的多位点序列分型研究[J]. 中国人兽共患病学报, 2013, 29(12):1137-1142.SUN H, BAI X N, ZHAO A L, et al. Multilocus sequence typing of Shiga toxin-producing Escherichia coli isolated from yaks[J]. Chinese Journal of Zoonoses, 2013, 29(12):1137-1142. (in Chinese)
[20]	杨育斌. 畜产品安全分析及应对措施[J]. 甘肃农业, 2004(1):34-35.YANG Y B. Animal product safety analysis and countermeasures[J]. Gansu Agriculture, 2004(1):34-35. (in Chinese)
[21]	张锦, 马宏, 夏胜利, 等. 产志贺毒素大肠杆菌毒力基因检测[J]. 中国公共卫生, 2003, 19(12):56-57.ZHANG J, MA H, XIA S L, et al. Detection of toxicity genes of Shiga toxigenic Escherichia coli[J]. Chinese Journal of Public Health, 2003, 19(12):56-57. (in Chinese)
[22]	薛涛, 李丽, 高清清, 等. 牛源产志贺毒素大肠杆菌分离株的毒力基因分布和遗传进化分析[J]. 中国畜牧兽医, 2017, 44(10):2878-2885.XUE T, LI L, GAO Q Q, et al. Study on the virulence gene distribution and genetic evolution of cattle Shiga toxin-producing Escherichia coli isolates[J]. China Animal Husbandry & Veterinary Medicine, 2017, 44(10):2878-2885. (in Chinese)
[23]	诸明欣, 张焕容, 汤承, 等. 四川阿坝州牦牛源溶血性大肠杆菌血清型鉴定及毒力相关基因检测[J]. 中国预防兽医学报, 2018, 40(1):9-13.ZHU M X, ZHANG H R, TANG C, et al. Serotype identification and virulence-related genes detection of hemolytic E. coli isolated from yak in Sichuan Aba state[J]. Chinese Journal of Preventive Veterinary Medicine, 2018, 40(1):9-13. (in Chinese)
[24]	张力国. 牛源大肠杆菌毒素基因、黏附素基因、O血清型及耐药性调查[D]. 哈尔滨:东北农业大学, 2015.ZHANG L G. Investigation of toxin gene, adhesin, O serotype and drug resistant of cattle Escherichia coli[D]. Harbin:Northeast Agricultural University, 2015. (in Chinese)
[25]	BAI X N, ZHANG W, TANG X Y, et al. Shiga toxin-producing Escherichia coli in Plateau Pika (Ochotona curzoniae) on the Qinghai-Tibetan Plateau, China[J]. Front Microbiol, 2016, 7:375.
[26]	陈迎春, 曹又方, 赵立平. 大肠杆菌MG1655菌株ERIC-PCR图谱主带序列组成分析[J]. 微生物学通报, 2002, 29(6):28-32.CHEN Y C, CAO Y F, ZHAO L P, et al. Non-random nature of genomic DNA amplification of E.coli K-12 MG1655 via ERIC-PCR[J]. Microbiology, 2002, 29(6):28-32. (in Chinese)
[27]	KUMAR R, SURENDRAN P K, THAMPURAN N. Molecular fingerprinting of Salmonella enterica subsp. enterica typhimurium and Salmonella enterica subsp. enterica Derby isolated from tropical seafood in South India[J]. Mol Biotechnol, 2008, 40(1):95-100.
[28]	LACIAR A, VACA L, LOPRESTI R, et al. DNA fingerprinting by ERIC-PCR for comparing Listeria spp. strains isolated from different sources in San Luis, Argentina[J]. Rev Argent Microbiol, 2006, 38(2):55-60.
[29]	BHOWMICK P P, KHUSHIRAMANI R, RAGHUNATH P, et al. Molecular typing of Vibrio parahaemolyticus isolated from seafood harvested along the south-west coast of India[J]. Lett Appl Microbiol, 2008, 46(2):198-204.
[30]	段会勇, 柴同杰, 蔡玉梅, 等. ERIC-PCR对鸡舍大肠杆菌气溶胶向舍外环境传播的鉴定[J]. 中国科学C辑:生命科学, 2008, 38(1):74-83.DUAN H Y, CHAI T J, CAI Y M, et al. Transmission identification of Escherichia coli aerosol in chicken houses to their environments using ERIC-PCR[J]. Science in China Series C:Life Sciences, 2008, 51(2):164-173.(in Chinese)
[31]	何力, 傅玲琳, 冯立芳, 等. 大肠埃希氏菌ERIC-PCR指纹图谱构建及贝类污染微生物源示踪[J]. 中国食品学报, 2012, 12(8):163-169.HE L, FU L L, FENG L F, et al. ERIC-PCR fingerprinting of fecal Escherichia coli and microbial source tracking in non-point pollution of the shellfish culture area of East China sea[J]. Journal of Chinese Institute of Food Science and Technology, 2012, 12(8):163-169. (in Chinese)
[32]	林朝洪. 猪源益生乳杆菌的分离、鉴定和筛选及ERIC-PCR分型研究[D]. 雅安:四川农业大学, 2007.LIN Z H. Identification, screening and ERIC-PCR differentiation of lactobacilli isolated from weaning piglet[J]. Ya'an:Sichuan Agricultural University, 2007. (in Chinese)
[33]	陈月月, 王凯, 王舒丰, 等. 新疆伊犁地区不同动物源大肠杆菌耐药性调查[J]. 新疆农业科学, 2018, 55(8):1560-1568.CHEN Y Y, WANG K, WANG S F, et al. Investigation into drug resistance of Escherichia coli from different animal sources in Yili, Xinjiang[J]. Xinjiang Agricultural Sciences, 2018, 55(8):1560-1568. (in Chinese)
[34]	邹立扣, 蒲妍君, 杨莉, 等. 四川省猪肉源大肠杆菌和沙门氏菌的分离与耐药性分析[J]. 食品科学, 2012, 33(13):202-206.ZOU L K, PU Y J, YANG L, et al. Isolation and drug resistance analysis of Escherichia coli and Salmonella spp. in pork from Sichuan province[J]. Food Science, 2012, 33(13):202-206. (in Chinese)

Detection and Analysis of Shiga Toxin-producing Escherichia coli Isolates from Cattle and Sheep Sources in Some Regions of Xinjiang, China

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 34

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHENG Rui, LIU Zishi, ZHANG Kangyou, YAN Yong, WEI Ling, ZEREN Wengmu, DINGZE Demi, HUANG Jianjun, WANG Li, WEI Yong. Isolation, Identification and Biological Characterization of Colletotrichum jasminigenum in Stems of Peanuts [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2206-2213.
[2]	ZHANG Yanmin, ZHAO Dongxu, WANG Wenlong. Mechanism of Resistance to Ivermectin in the Haemonchus contortus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1511-1520.
[3]	SONG Yan, YUAN Yongfeng, QIAN Hongyu, LI Xincan, LUO Hongyan, WANG Zhiying, ZHOU Zuoyong. Identification and Partial Biological Characteristics Analysis of Corynebacterium pseudotuberculosis Isolated from Goats [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 680-687.
[4]	LIU Xinhuan, YUN Jialei, MAO Li, LI Jizong, HAO Fei, HE Miaofeng, YANG Leilei, ZHANG Wenwen, CHENG Zilong, SUN Min, LIU Maojun, WANG Shaohui, BAI Juan, LI Wenliang. Isolation, Identification, Virulence Genes and Drug Resistance Analysis of Escherichia coli Isolated from Diarrheal Goat and Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3445-3454.
[5]	ZHAO Feifei, LI Jie, HAN Ning, XIE Shiting, ZENG Zhenling. Antibacterial Drug Resistance Analysis of Klebsiella pneumoniae Isolated from Slaughterhouse [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 3044-3053.
[6]	JIANG Zenghai, TENG Lin, HE Anwen, LIU Yanyan, YUE Min, HE Qigai. Genomic Analysis of Salmonella Typhimurium Isolates and Salmonella Serotype 4, [5], 12: i:- Isolates from Pig-borne Food Chain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1199-1209.
[7]	Lü Ruoyi, SI Xiaohui, SUN Zhigang, SHI Xiaomin, LIU Xiaoye. Drug Resistance Situation of Streptococcus suis and Prevention Measures of Infections [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4920-4933.
[8]	WANG Jianing, ZHANG Ziqiang, KONG Dejing, FENG Caicai, ZHANG Feike, LIU Yumei. Isolation and Identification of Klebsiella pneumoniae in Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5198-5206.
[9]	YANG Menglin, ZHENG Shiqi, PENG Kai, WANG Wei, HUANG Yanhua, PENG Jie. Isolation and Identification of Pigeon-derived Salmonella Typhimurium and Pathogenic Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4880-4888.
[10]	ZHANG Kaichuan, WANG Jinyu, LI Shoujun, JIA Kun. Isolation, Identification and Biological Characteristics of Klebsiella pneumoniae from Sheep in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 328-337.
[11]	TONG Panpan, HUANG Shunmin, WANG Yudan, SHI Xuhui, CHEN Wenxia, SONG Xinlong, ZHANG Yi, SU Zhanqiang, XIE Jinxin. Phylogenetic Clustering, Serotype and Drug Resistance Analysis of Escherichia coli from Diarrhea with Piglets in Xinjiang [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 414-420.
[12]	YU Yongfeng, QUAN Heng, DONG Wenhao, ZOU Ronghua, WU Xiaoni, GONG Xiaowei, CHEN Qiwei. The Mechanism of Two-component Regulatory System Mediating Drug Resistance of Gram-negative Bacteria [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1689-1701.
[13]	WANG Xi, LI Ke, LI Tingcui, YAN Hongya, ZHAO Rong, CHANG Zhishun, LIAO Ming, SUN Minhua, XIN Aiguo. MLST Typing and Drug Resistance Analysis of 75 Salmonella Strains Isolated from Laying Hens [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1626-1631.
[14]	ZHAO Xueliang, WANG Bin, MIAO Yongqiang, ZHAO Haoyu, XIE Qingfang, WANG Juan, YANG Zengqi. Detection of Virulence Genes and Antimicrobial Resistance Analysis of Escherichia coli Isolated from Diarrhea Sheep in Shaanxi Province [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1644-1648.
[15]	ZHANG Lin, LU Fang, FU Hengfeng, JIANG Xidi, WEI Qiling, QI Caili, GAO Haixia, LI Lin. Regulation Mechanism of BaeSR on Fluoroquinolones Resistance in Salmonella Typhimurium [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(3): 894-903.