新疆部分地区牛羊源产志贺毒素大肠埃希菌菌株检测与分析

doi:10.11843/j.issn.0366-6964.2020.10.020

Abstract

Abstract: Shiga toxin-producing Escherichia coli(STEC) is a new class of highly pathogenic food-borne pathogens carrying a prephage encoding one or two Shiga toxin genes. It has become an important public health issue that threatens human health. The present work aimed to characterize STEC strains isolated from cattle and sheep at various stages, in parts of Xinjiang, in terms of the presence of prevalence, genetic diversity, and antimicrobial susceptibility to 17 common antibiotics. Through amplification of four virulence genes (stx1, stx2, eae, hlyA)by PCR and ERIC-PCR genotyping to detection STEC isolates. In the present study, a total of 64 STEC strains were isolated from 431 samples from slaughterhouses, farms and markets. Of these, 31 (48.4%) of the isolates harbored stx1 + stx2, and only 29 (45.3%) of the isolates possessed stx1, only 4 (6.3%) of the isolates harbored stx2, and 1 isolates harbored all the 4 virulence genes. Drug sensitivity tests found that STEC strains displayed 7 antimicrobial resistance to midecamycin(61%), cephalothin(4.7%), cefoxitin(4.7%), ampicillin(3.1%), piperacillin(1.6%), tobramycin(1.6%), cefazolin(1.6%). The ERIC-PCR results showed a polymorphic distribution, which was divided into two clusters of A (36 strains) and B (28 strains). STEC strains isolated from cattle and sheep at various stages, in parts of Xinjiang, some of which might have the potential to cause food contamination and human diseases.

Key words: Shiga toxin-producing Escherichia coli, virulence gene, drug resistance, ERIC-PCR

CLC Number:

S852.61

ZHENG Xiaofeng, ZHANG Yan, LIU Yingyu, ZHU Mingyue, ZHENG Xiaoqin, LU Wei, JIANG Jindou, ZHU Menghan. Detection and Analysis of Shiga Toxin-producing Escherichia coli Isolates from Cattle and Sheep Sources in Some Regions of Xinjiang, China[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2518-2527.

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Detection and Analysis of Shiga Toxin-producing Escherichia coli Isolates from Cattle and Sheep Sources in Some Regions of Xinjiang, China

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