雌激素通过ER-α调控CYP2C8在鸡肝中的表达

doi:10.11843/j.issn.0366-6964.2016.12.005

Abstract

Abstract:

The objectives of the present study were to investigate the characteristics of expression and regulation of CYP2C8 gene in chicken. A total of 14 tissues (including heart, liver, spleen, lung, kidney, pectoral muscle, abdominal fat, gizzard, glandular stomach, ovaries, pancreas, duodenum, jejunum and ileum) were collected from Lushi green-shelled-egg chickens at the age of 30 weeks old, and the liver tissues were obtained from the chickens at different development stages (from the age of 1 day to 30 weeks old) . The samples were used to detect the spatiotemporal expression profiles of CYP2C8 gene using quantitative real-time PCR (qRT-PCR) technology. In addition, the pullets (at the age of 10 weeks old) which were treated with 17β-estradiol, and chicken embryo primary hepatocytes which were treated with either 17β-estradiol alone or combination of 17β-estradiol and estrogen receptor (ER) antagonists were used to analysis the expression regulation mechanism of the CYP2C8 gene. The results showed that CYP2C8 gene was expressed extensively in different tissues of chicken. The relative expression level of CYP2C8 was higher in the main lipid metabolism tissues such as liver, kidney, and small intestine (duodenum, jejunum and ileum) than that in other tissues. The expression level of CYP2C8 gene in liver of hens was significantly increased with the sexual maturity (P<0.01). Estrogen could significantly up-regulate the expression of CYP2C8 in liver of chicken and hepatocytes (P<0.05). MPP, as an ER-α specific antagonist, could completely antagonize the expression of CYP2C8 gene (P<0.05). Therefore we concluded that CYP2C8 gene was expressed extensively in different tissues with higher expression levels in liver, kidney, and small intestine (duodenum, jejunum, and ileum) tissues, and the highest expression level in liver. It was suggested that the effect of estrogen on the expression of CYP2C8 gene was predominantly mediated via ER-α in the chicken liver.

CLC Number:

S831.2

LI Yan-min, LI Cui-cui, ZHENG Hang, TIAN Fang-yuan, WANG Tai-an, TIAN Ya-dong, LI Zhuan-jian, KANG Xiang-tao,LIU Xiao-jun. Expression of CYP2C8 Is Regulated by Estrogen through ER-α in Liver of Chicken[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(12): 2362-2369.

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Expression of CYP2C8 Is Regulated by Estrogen through ER-α in Liver of Chicken

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