IHH过表达对鸡原代软骨细胞增殖和分化的影响

doi:10.11843/j.issn.0366-6964.2023.11.013

Abstract

Abstract: The study aimed to explore the regulatory effect of the Indian hedgehog (IHH) gene on the proliferation and differentiation of chicken primary chondrocytes. The eukaryotic overexpression vector (OV-IHH) was constructed according to the sequence information of the IHH gene in this study. The OV-IHH vector was identified by double enzyme digestion and sequencing, and transfected into chicken primary chondrocytes. The cell proliferation was detected by CCK-8 method, and the cell cycle and apoptosis were detected by flow cytometry. The chondrocytes were induced to differentiate into osteoblasts for 21 d. The ALP activity of cells was detected by the ALP kit. The results showed that the IHH eukaryotic overexpression vector was successfully constructed. Compared with the control group and the empty vector group (OV-NC), the mRNA expression of the IHH gene in the overexpression group was significantly increased (P<0.01), and the overexpression efficiency was increased about 2 700 times. Moreover, the overexpression of IHH promoted the transformation of the cell cycle from G1 to S phase, thereby promoting proliferation and inhibiting apoptosis, increasing ALP activity, and promoting cell differentiation. In conclusion, the IHH gene significantly affects the growth and development of chicken chondrocytes, which provides a theoretical basis for further analysis of the mechanism of the IHH gene regulating the creeper trait at the cellular level.

Key words: IHH, chicken primary chondrocytes, overexpression, proliferation, differentiation

CLC Number:

S831.2

JIN Sihua, JIA Fumin, HE Peili, JIA Yuqing, SHUI Fei, LIU Xuling, LIU Xing, WANG Xin, XU Man, GENG Zhaoyu. Effect of IHH Gene Overexpression on Proliferation and Differentiation of Chicken Primary Chondrocytes[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4569-4576.

References 20

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Effect of IHH Gene Overexpression on Proliferation and Differentiation of Chicken Primary Chondrocytes

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