琅琊鸡FSHR基因-868位点的多态性及对产蛋性能的遗传效应研究

doi:10.11843/j.issn.0366-6964.2023.11.012

Abstract

Abstract: The study aimed to analyze the polymorphism and genetic effects of -868 locus of FSHR gene in Langya chickens. Genotyping of the -868 locus of FSHR gene were performed by PCR amplification and electrophoresis on 382 Langya hens that were raised with the same feed management and were recorded individually for egg performance, and the correlation between the locus and egg laying traits was analyzed. Quantitative real-time PCR (qRT-PCR) and Western blotting were used to compare mRNA and protein expression levels of chicken FSHR. The results showed that, the polymorphism of -868 locus in FSHR 5' regulatory region in the Langya chicken population was found, I^-was the dominant allele, and this population deviated from the Hardy-Weinberg equilibrium (P<0.05). The egg number at 47 weeks (E47) and the egg number at 134 days after molting (134D) were both significantly higher for hens with I⁺I⁺ than those with I^-I^- (P<0.05). The maximum clutch size (MCS) was significantly higher for hens with I⁺I⁺ and I⁺I^- than those with I^-I^- (P<0.01). The expression of FSHR mRNA in pre-hierarchical follicles was significantly higher for hens with genotype I⁺I⁺ than those with I^-I^-(P<0.05). The expression of FSHR protein in the ovary was significantly higher for hens with genotype I⁺I^- than those with I^-I^-(P<0.05). There were polymorphism at the -868 locus of FSHR gene in Langya chickens, the genetic effect of genotypes on E47, MCS and 134D was significant and hens with genotype I⁺I⁺ exhibited higher expression level of FSHR. In Langya chicken breeding, egg laying performance is expected to increase by decreasing the allele frequency of I^-.

Key words: Langya chicken, FSHR, -868 locus polymorphism, egg laying traits, expression

CLC Number:

S831.2

CHEN Cheng, QIAO Xibo, SUN Yi, KANG Li, JIANG Yunliang. Study on the Polymorphism and Genetic Effect of the -868 Locus of FSHR Gene on Egg Laying Traits in Langya Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4560-4568.

References 39

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Study on the Polymorphism and Genetic Effect of the -868 Locus of FSHR Gene on Egg Laying Traits in Langya Chickens

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