营养转运相关基因调控区多态性与黄羽肉鸡饲料转化率关联分析

doi:10.11843/j.issn.0366-6964.2023.06.014

Abstract

Abstract: The study aimed to investigate the polymorphism of regulatory regions of several nutrition transport-related genes and screen the key variations affecting the feed conversion ratio of broilers. The blood DNA samples of 439 male yellow-feather broilers were collected in this study, and the polymorphic loci within 1 kb upstream of 3 monosaccharide transporter genes (GLUT2, GLUT5 and SGLT1), 8 amino acid transporter genes (B⁰AT1, B⁰⁺AT, CAT1, CAT2, LAT1, y⁺LAT1, y⁺LAT2 and rBAT) and 1 nutrient regulation gene (FGF1) were detected in the two-tail sample group with high and low feed conversion ratio by PCR sequencing. According to the difference in genotype frequency in the two-tail sample groups, the genetic variations with potential influence on feed conversion ratio were screened. The PCR-RFLP method was used to genotype the polymorphic loci in the whole sample and the association between these polymorphic loci with feed conversion ratio and other traits was analyzed. The results showed that a total of 111 SNPs sites were found within 1 kb upstream of 12 genes in this study, among which 8 SNPs sites had different genotype frequencies in the two-tail sample groups,including -379A>G of GLUT2, -490G>A of SGLT1, -639T>C of B⁰AT1, -46G>A, -90G>T, -180T>C, -235G>A of rBAT, and -884T>C of FGF1. Further whole population genotyping and association analysis showed that the -639T>C of B⁰AT1 was significantly associated with feed conversion ratio (P<0.05), individuals with TC genotype had significantly lower feed conversion ratio than those with TT and CC genotypes (P<0.05), showed a heterozygous advantage. The -884T>C of FGF1 was also significantly associated with feed conversion ratio (P<0.05), and the individuals with CC and TT genotypes had significantly lower feed conversion ratio than those with the TC genotype (P<0.05). In addition, the -379A>G of GLUT2 was not significantly associated with feed conversion or growth traits. In this study, the genetic variation of regulatory regions of several nutrient transport-related genes was analyzed, which provides the basis for using these variation sites as genetic markers to improve the feed conversion ratio of yellow-feather broilers.

Key words: yellow-feather boiler, feed conversion ratio, association study, nutrient transport

CLC Number:

S831.2

WANG Zheng, GUO Wenjie, CHENG Jin, YUAN Yitong, LUO Rong, XUE Yi, ZHANG Lihuan, ZHU Zhiwei, LI Huifeng. Association Study of Polymorphism in the Regulatory Region of Nutrient Transport-related Genes and Feed Conversion Ratio in Yellow-feather Broiler[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2343-2352.

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Association Study of Polymorphism in the Regulatory Region of Nutrient Transport-related Genes and Feed Conversion Ratio in Yellow-feather Broiler

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