Wnt3a基因多态性与崇仁麻鸡皮肤毛囊性状相关性研究

doi:10.11843/j.issn.0366-6964.2023.07.014

Abstract

Abstract: The aim of this study was to analyze the correlation between genetic variant loci of Wnt family member 3a (Wnt3a) gene and skin hair follicle density in Chongren partridge chicken based on gene polymorphism.Six hundred Chongren partridge chickens, 200 protospecies hens and 100 roosters and 200 B strain hens and 100 roosters, reared under the same environment and the same management level until date of listing (120 d), were randomly selected in good health and condition. After slaughter, hair follicle density was measured at the same locations on the skin of the back, chest and thighs, the morphological structure of hair follicles was observed by HE staining of skin tissue sections, and the diameter of hair follicles and skin thickness were measured. The genetic variant loci of Wnt3a gene were screened by PCR amplification followed by direct sequencing, and their correlation with the hair follicle density of chicken skin was analyzed. The expression of Wnt3a gene in different skin sites was detected by real-time fluorescence quantitative PCR technique. The results showed that:1) Comparing the same sex and skin area of protospecies and B strain, the hair follicle density on the back of protospecies hens was extremely significantly higher than that of B strain (P<0.01), the hair follicle density on the thighs was significantly higher than that of B strain (P<0.05), and the hair follicle diameter on the back and thighs was significantly lower than that of B strain (P<0.05). The density of skin follicles on the back of the protospecies cock was highly significant higher than that of B strain (P<0.01), the density of hair follicles on the chest was highly significant lower than that of B strain (P<0.01), and the diameter of hair follicles on the back and thighs was significantly lower than that of B strain (P<0.05).The thickness of the skin on the chest of males in B strain was signifi-cantly higher than that of protospecies (P<0.05), and the thickness of the skin on the thighs of both males and females in B strain was significantly higher than that of protospecies (P<0.05). 2) Three SNPs loci were screened for the Wnt3a gene and their correlation with the skin follicle density at each site was analyzed. g.2555812 T>C and g.2555377 T>C loci were found to have significantly higher skin follicle density on the back of individuals with CC genotype than that of individuals with other genotypes (P<0.05). 3) Real-time fluorescence quantitative PCR results showed that the Wnt3a gene in hair follicle tissues of all 3 skin sites of the protospecies and the B strain had relatively high expression, and the expression in the dorsal part of the protospecies was significantly higher than that in the dorsal part of the B strain (P<0.05). In conclusion, the skin pores of protospecies of Chongren partridge chicken were finer compared to the B strain, and the CC genotype of the Wnt3a gene at the g.2555812 T>C locus and the CC genotype at the g.2555377 T>C locus were favorable genotypes for the skin follicles of Chongren partridge chicken and could be used as molecular markers for the chicken skin follicle density trait. In this study, we investigated the genetic effects of Wnt3a as a candidate gene for hair follicle growth and develop-ment and the correlation between SNPs and skin hair follicle traits, in order to provide a reference basis for molecular marker screening and marker-assisted selection of quality chicken carcass packaging traits.

Key words: Chongren partridge chicken, hair follicle traits, Wnt3a gene, SNP loci, fluorescence quantification

CLC Number:

S831.2

CHEN Chun, KANG Zhaofeng, WEI Yue, LI Guanhong, WU Yanping, XIE Jinfang. Correlation of Wnt3a Gene Polymorphism with Skin Follicle Traits in Chongren Partridge Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2810-2823.

References 45

[1]	周勇, 李知新, 鲁宏伟, 等.我国H5和H7N9亚型高致病性禽流感的监测及疫情暴发分析[J].畜牧兽医学报, 2022, 53(9):3093-3106.ZHOU Y, LI Z X, LU H W, et al.Surveillance and outbreak analysis of H5 and H7N9 subtypes of highly pathogenic avian influenza in China[J].Acta Veterinaria et Zootechnica Sinica, 2022, 53(9):3093-3106.(in Chinese)
[2]	ZENG H, DE REU K, GABRIËL S, et al.Salmonella prevalence and persistence in industrialized poultry slaughterhouses[J]. Poult Sci, 2021, 100(4):100991.
[3]	刘国信.转变消费观念推广"冰鲜鸡"是大势所趋[J].养禽与禽病防治, 2016(11):34-36.LIU G X.Change the concept of consumption, the promotion of "chilled chicken" is the trend[J].Poultry Husbandry and Disease Control, 2016(11):34-36.(in Chinese)
[4]	WANG X S, TREDGET E E, WU Y J.Dynamic signals for hair follicle development and regeneration[J].Stem Cells Dev, 2012, 21(1):7-18.
[5]	LEE J, RABBANI C C, GAO H Y, et al.Hair-bearing human skin generated entirely from pluripotent stem cells[J].Nature, 2020, 582(7812):399-404.
[6]	LI G, TANG X L, ZHANG S P, et al.SIRT7 activates quiescent hair follicle stem cells to ensure hair growth in mice[J].EMBO J, 2020, 39(18):e104365.
[7]	XIE W Y, CHEN M J, JIANG S G, et al.Investigation of feather follicle morphogenesis and the expression of the Wnt/β-catenin signaling pathway in Yellow-feathered broiler chick embryos[J].Br Poult Sci, 2020, 61(5):557-565.
[8]	WU P, JIANG T X, LEI M X, et al.Cyclic growth of dermal papilla and regeneration of follicular mesenchymal components during feather cycling[J].Development, 2021, 148(18):dev198671.
[9]	GEYFMAN M, PLIKUS M V, TREFFEISEN E, et al.Resting no more:re-defining telogen, the maintenance stage of the hair growth cycle[J].Biol Rev Camb Philos Soc, 2015, 90(4):1179-1196.
[10]	STENN K S, PAUS R.Controls of hair follicle cycling[J].Physiol Rev, 2001, 81(1):449-494.
[11]	JI S F, ZHU Z Y, SUN X Y, et al.Functional hair follicle regeneration:an updated review[J].Signal Transduct Target Ther, 2021, 6(1):66.
[12]	HARDY M H.The secret life of the hair follicle[J].Trends Genet, 1992, 8(2):55-61.
[13]	SHANG F Z, WANG Y, MA R, et al.Screening of microRNA and mRNA related to secondary hair follicle morphogenesis and development and functional analysis in cashmere goats[J].Funct Integr Genomics, 2022, 22(5):835-848.
[14]	KIRIKOSHI H, SEKIHARA H, KATOH M.Expression of WNT14 and WNT14B mRNAs in human cancer, up-regulation of WNT14 by IFNγ and up-regulation of WNT14B by β-estradiol[J].Int J Oncol, 2001, 19(6):1221-1225.
[15]	GUO H Y, YANG K, DENG F, et al.Wnt3a inhibits proliferation but promotes melanogenesis of melan-a cells[J].Int J Mol Med, 2012, 30(3):636-642.
[16]	GUO H Y, XING Y Z, LIU Y X, et al.Wnt/β-catenin signaling pathway activates melanocyte stem cells in vitro and in vivo[J].J Dermatol Sci, 2016, 83(1):45-51.
[17]	TANG M S, WANG T, ZHANG X F.A review of SNP heritability estimation methods[J].Brief Bioinform, 2022, 23(3):bbac067.
[18]	束婧婷, 姬改革, 屠云洁, 等.鸡Wnt3a基因组织表达差异与SNPs对鸡毛囊密度性状的遗传效应[J].中国畜牧兽医, 2021, 48(5):1672-1680.SHU J T, JI G G, TU Y J, et al.Tissue expression analysis of Wnt3a gene and genetic effects of SNPs on feather follicle density in chicken[J].China Animal Husbandry & Veterinary Medicine, 2021, 48(5):1672-1680.(in Chinese)
[19]	吴静文.鸡皮肤黄度的遗传规律及相关分子标记的鉴定[D].广州:华南农业大学, 2020.WU J W.Characterization of chicken skin yellowness and ldentification of related molecular markers[D].Guangzhou:South China Agricultural University, 2020.(in Chinese)
[20]	YANG X, XIAN Y R, LI Z H, et al.G0S2 gene polymorphism and its relationship with carcass traits in chicken[J].Animals (Basel), 2022, 12(7):916.
[21]	SUN G R, LI M, LI H, et al.Molecular cloning and SNP association analysis of chicken PMCH gene[J].Mol Biol Rep, 2013, 40(8):5049-5055.
[22]	赵振华, 黎寿丰, 黄华云, 等.优质肉鸡胴体外观性状遗传力及相关性分析[J].四川农业大学学报, 2015, 33(1):89-92.ZHAO Z H, LI S F, HUANG H Y, et al.Estimates of heritability and correlations for the carcass appearance performance of high-quailty broiler chickens[J].Journal of Sichuan Agricultural University, 2015, 33(1):89-92.(in Chinese)
[23]	SCHNEIDER M R, SCHMIDT-ULLRICH R, PAUS R.The hair follicle as a dynamic miniorgan[J].Curr Biol, 2009, 19(3):R132-R142.
[24]	YU M K, WU P, WIDELITZ R B, et al.The morphogenesis of feathers[J].Nature, 2002, 420(6913):308-312.
[25]	姬改革, 束婧婷, 单艳菊, 等.鸡皮肤毛囊性状研究进展[J].中国家禽, 2019, 41(10):46-49.JI G G, SHU J T, SHAN Y J, et al.Research progress of skin hair follicle traits in chicken[J].China Poultry, 2019, 41(10):46-49.(in Chinese)
[26]	RISHIKAYSH P, DEV K, DIAZ D, et al.Signaling involved in hair follicle morphogenesis and development[J].Int J Mol Sci, 2014, 15(1):1647-1670.
[27]	SONG Y P, LIU C, ZHOU Y X, et al.Regulation of feather follicle development and Msx2 gene SNP degradation in Hungarian white goose[J].BMC Genomics, 2022, 23(1):821.
[28]	JENNI L, GANZ K, MILANESI P, et al.Determinants and constraints of feather growth[J].PLoS One, 2020, 15(4):e0231925.
[29]	RIM E Y, CLEVERS H, NUSSE R.The Wnt pathway:from signaling mechanisms to synthetic modulators[J].Annu Rev Biochem, 2022, 91:571-598.
[30]	CHATTERJEE A, PAUL S, BISHT B, et al.Advances in targeting the WNT/β-catenin signaling pathway in cancer[J].Drug Discov Today, 2022, 27(1):82-101.
[31]	SICK S, REINKER S, TIMMER J, et al.WNT and DKK determine hair follicle spacing through a reaction-diffusion mechanism[J].Science, 2006, 314(5804):1447-1450.
[32]	李瑶, 孙远, 鲍忠省, 等.Wnt5a基因在鹅皮肤中的定量定位表达特性[J].吉林农业大学学报, 2014, 36(4):475-478, 493.LI Y, SUN Y, BAO Z S, et al.Characteristics of quantitative and positioning expression of Wnt5a Gene in Goose Skin[J].Journal of Jilin Agricultural University, 2014, 36(4):475-478, 493.(in Chinese)
[33]	XING Y Z, MA X G, GUO H Y, et al.Wnt5a Suppresses β-catenin Signaling during Hair Follicle Regeneration[J].Int J Med Sci, 2016, 13(8):603-610.
[34]	KANDYBA E, KOBIELAK K.Wnt7b is an important intrinsic regulator of hair follicle stem cell homeostasis and hair follicle cycling[J].Stem Cells, 2014, 32(4):886-901.
[35]	TAO Y F, ZHOU X L, LIU Z W, et al.Expression patterns of three JAK-STAT pathway genes in feather follicle development during chicken embryogenesis[J].Gene Expr Patterns, 2020, 35:119078.
[36]	XIE W Y, CHEN M J, JIANG S G, et al.The Wnt/β-catenin signaling pathway is involved in regulating feather growth of embryonic chicks[J].Poult Sci, 2020, 99(5):2315-2323.
[37]	HU X W, ZHANG X K, LIU Z W, et al.Exploration of key regulators driving primary feather follicle induction in goose skin[J].Gene, 2020, 731:144338.
[38]	ASADOLLAHPOUR NANAEI H, KHARRATI-KOOPAEE H, ESMAILIZADEH A.Genetic diversity and signatures of selection for heat tolerance and immune response in Iranian native chickens[J].BMC Genomics, 2022, 23(1):224.
[39]	ZHUANG Z X, CHEN S E, CHEN C F, et al.Genomic regions and pathways associated with thermotolerance in layer-type strain Taiwan indigenous chickens[J].J Therm Biol, 2020, 88:102486.
[40]	GILLEN S L, WALDRON J A, BUSHELL M.Codon optimality in cancer[J].Oncogene, 2021, 40(45):6309-6320.
[41]	BAILEY S F, MORALES L A A, KASSEN R.Effects of synonymous mutations beyond codon bias:the evidence for adaptive synonymous substitutions from microbial evolution experiments[J].Genome Biol Evol, 2021, 13(9):evab141.
[42]	PARVATHY S T, UDAYASURIYAN V, BHADANA V.Codon usage bias[J].Mol Biol Rep, 2022, 49(1):539-565.
[43]	CALLENS M, PRADIER L, FINNEGAN M, et al.Read between the Lines:diversity of nontranslational selection pressures on local codon usage[J].Genome Biol Evol, 2021, 13(9):evab097.
[44]	PANARO M A, CALVELLO R, MINIERO D V, et al.Imaging intron evolution[J].Methods Protoc, 2022, 5(4):53.
[45]	刘继强, 郝晓东, 武丽娜, 等.全基因组SNP分型技术在畜禽遗传育种研究中的应用[J].畜牧兽医学报, 2022, 53(12):4123-4137.LIU J Q, HAO X D, WU L N, et al.Application of whole genome SNP genotyping technology in livestock and poultry genetics and breeding[J].Acta Veterinaria et Zootechnica Sinica, 2022, 53(12):4123-4137.(in Chinese)

Correlation of Wnt3a Gene Polymorphism with Skin Follicle Traits in Chongren Partridge Chicken

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 45

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TANG Xinxin, ZHENG Jumei, LUO Na, YING Fan, ZHU Dan, LI Sen, LIU Dawei, AN Bingxing, WEN Jie, ZHAO Guiping, LI Hegang. Genetic Mechanism of Broiler Leg Disease Based on Genome-Wide Association Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 99-109.
[2]	XU Xiaojing, DONG Ruiling, WEI Limin, ZHAO Shaomeng, ZHAO Guiping, ZHANG Minhong, FENG Jinghai. Study on Growth Model of Wenchang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4952-4961.
[3]	QI Lina, LU Xuelin, YANG Kaixuan, ZHOU Jin, LI Xianyu, LIU Ke, ZHANG Wengang, GU Xin, ZHANG Weijian. Analysis of Genetic Diversity and Genetic Structure of New Pudong Chicken Based on SNP Chips [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4962-4971.
[4]	WANG Yanxing, ZHANG Yushi, JI Haigang, LIU Yang, NIU Yufang, HAN Ruili, LIU Xiaojun, TIAN Yadong, KANG Xiangtao, LI Zhuanjian. Establishment and Analysis of the Chicken Skeletal Satellite Cell Line [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4972-4981.
[5]	FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.
[6]	CHEN Cheng, QIAO Xibo, SUN Yi, KANG Li, JIANG Yunliang. Study on the Polymorphism and Genetic Effect of the -868 Locus of FSHR Gene on Egg Laying Traits in Langya Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4560-4568.
[7]	JIN Sihua, JIA Fumin, HE Peili, JIA Yuqing, SHUI Fei, LIU Xuling, LIU Xing, WANG Xin, XU Man, GENG Zhaoyu. Effect of IHH Gene Overexpression on Proliferation and Differentiation of Chicken Primary Chondrocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4569-4576.
[8]	CHEN Xuejiao, LIU Huijie, ZANG Lei, FENG Jing, PENG Da, ZHANG Hao. Transcriptome Data from Chicken Embryo Heart Tissue Identified Key Genes for Altitude Hypoxia Adaptation in Xueyu White Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4154-4163.
[9]	ZHANG Yinliang, YUE Qiaoxian, HUANG Chenxuan, CHEN Hui, WANG Dehe, ZHOU Rongyan. Construction of Tibia Transcript Profile of Laying Hens at the Early and Late Laying Stages and Analysis of Genes Related to Bone Metabolism [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4164-4173.
[10]	ZHENG Gang, LIAN Ling. Research Progress of Key Regulatory Gene DMRT1 in Chicken Sex Determination and Differentiation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3152-3163.
[11]	BAI Lu, WANG Mengjie, MA Xiaochun, HE Zhengxiao, TAN Xiaodong, LIU Jie, ZHAO Guiping, WEN Jie, LIU Ranran. A Method for Quickly Mining the Characteristic SNP Markers Set of Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3252-3261.
[12]	LIU Hang, WANG Huanhuan, GE Ying, ZHANG Lei, ZHANG Weiwu, WEI Yinghui, LI Qinghai, FAN Jinghui, ZHANG Xuedong. Screening of Candidate Genes of Skin Color of Black-Bone Chicken Based on Transcriptome and Proteome [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2320-2329.
[13]	WANG Hui, XU Mengyuan, LIU Lingkang, ZHENG Xibang, LI Gonghe, WU Wende. PhiC31 Integrase Triggers Site-specific Cassette Exchange in Chicken Fibroblast Cells through Electroporation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2330-2342.
[14]	WANG Zheng, GUO Wenjie, CHENG Jin, YUAN Yitong, LUO Rong, XUE Yi, ZHANG Lihuan, ZHU Zhiwei, LI Huifeng. Association Study of Polymorphism in the Regulatory Region of Nutrient Transport-related Genes and Feed Conversion Ratio in Yellow-feather Broiler [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2343-2352.
[15]	WANG Zixuan, WANG Qiao, ZHANG Jin, Astrid Lissette Barreto Sánchez, ZHENG Maiqing, LI Qinghe, CUI Huanxian, AN Bingxing, ZHAO Guiping, WEN Jie, LI Hegang. Transcriptome Based Screening of Functional Genes Related to Heat Stress Resistance in Beijing You Chickens and Guangming Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1905-1914.