ACTA VETERINARIA ET ZOOTECHNICA SINICA

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Genetic Variation Analysis of ORF5 Gene of 8 Porcine Reproductive and Respiratory Syndrome Virus Isolates

LIU Ming-yang1,2, ZHANG Gai-ping2, HAO Hui-fang2, QIAO Song-lin2,WAN Bo2, BAO Deng-ke2, GUO Cheng-liu2, WANG Ai-ping1*   

  1. 1. Department of Bioengineering, Zhengzhou University, Zhengzhou 450001, China; 2. Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratoryof Animal Immunology, Henan Academy of Agricultural Science, Zhengzhou 450002, China
  • Received:2012-09-13 Online:2013-04-23 Published:2013-04-23

Abstract:

Real time monitoring information was analyzed to understand the genetic and pathogenic evolution of porcine reproductive and respiratory syndrome virus (PRRSV) in Central China during 2011-2012. RT-PCR was conducted to identify the discontinuous 29-amino acid deletion in the Nsp2 gene, which is the characteristic of highly-pathogenetic PRRSV (HP-PRRSV). The GP5 gene of 8 PRRSV isolates were cloned, sequenced and subsequently compared with that of 62 typical isolates deposited in GenBank over the years. The results showed that all the 8 isolates collected in Central China during 2011-2012 were closely related to the HP-PRRSV emerged in Southern China in 2006.

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