MID2对猪繁殖与呼吸综合征病毒复制的调控作用

doi:10.11843/j.issn.0366-6964.2023.11.027

Abstract

Abstract: The purpose of this experiment was to study the regulatory effect of MID2 on porcine reproductive and respiratory syndrome virus (PRRSV) replication. Firstly, MARC-145 cells were infected with BJ-4 strain of PRRSV, and then the mRNA and protein expression level of MID2 were detected by RT-qPCR and Western blot. It was found that PRRSV infection could up-regulate MID2 expression. To further evaluate the effect of MID2 on PRRSV replication, this study overexpressed or knocked down the expression level of MID2 protein, and then inoculated PRRSV. It was found that MID2 could inhibit the replication of PRRSV by detecting the transcription level of PRRSV ORF7 and the expression level of N protein. Furthermore, the pCMV-Flag-MID2 and pGL3-IFN-β-Luc plasmid were co-transfected into HEK293T cells, the result of double luciferase assay showed that MID2 could positively regulate the type I interferon signaling pathway. Finally, the plasmid of MID2 and RIG-I were co-transfected into HEK293T cells, the result of Co-IP assay indicated that MID2 could interact with RIG-I, and MID2 could promote the protein expression level of RIG-I in a dose-dependent manner and enhance RIG-I mediated I-IFN signaling pathway. These results suggested that MID2 could activate the type I IFN signaling pathway through interacting with RIG-I and promoting RIG-I expression, lead to inhibiting PRRSV replication. In this study, we found for the first time that MID2 can positively regulate the type I interferon signaling pathway and inhibit PRRSV replication.

Key words: MID2, porcine reproductive and respiratory syndrome virus, I-IFN, RIG-I

CLC Number:

S852.659.6

ZHOU Likun, DONG Xinyuan, LI Jiahui, CUI Zhiying, ZHAO Shijie, XU Jie, CHEN Jing, ZHANG Yina, XIA Ping’an. Regulatory Role of MID2 on Porcine Reproductive and Respiratory Syndrome Virus Replication[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4735-4744.

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Regulatory Role of MID2 on Porcine Reproductive and Respiratory Syndrome Virus Replication

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