鸡传染性支气管炎病毒感染HD11细胞的转录组分析

doi:10.11843/j.issn.0366-6964.2023.11.039

Abstract

Abstract: In order to investigate the innate immune response and antiviral response of host cells at the early stage of infection, infectious bronchitis virus (IBV) Beaudette strains was used to infect chicken macrophage HD11 cells. After 2 h, total RNA was extracted from the cells into cDNA, and high throughput transcriptomic sequencing was performed by Illumina. The results showed that 1 363 up-regulated genes and 653 down-regulated genes were screened out in the infection group. According to GO and KEGG analysis, it was found that in the early stage of the infection of IBV-infected HD11 cells, the differentially expressed genes were mostly related to immune response and antiviral effect. The enriched signaling pathways mainly focus on the cytokine-cytokine receptor interaction, viral protein interaction with cytokine and cytokine receptor, TNF signaling pathway, MAPK signaling pathway, etc. Ten randomly selected genes related to immune response and antiviral were verified by RT-qPCR, and the results were consistent with the transcriptome sequencing analysis. This study lays a foundation for further investigation of the pathogenesis of IBV and host cell immune response in the early stage of infection.

Key words: infectious bronchitis virus, chicken macrophage HD11, high-throughput sequencing, transcriptome

CLC Number:

CHEN Lu, CHANG Xinyu, SHEN Jiachen, SHEN Ruiting, ZHAO Zhenhua, HOU Xiaolin. Transcriptome Sequencing and Analysis of HD11 Cells Infected by Avian Infectious Bronchitis Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4860-4865.

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Transcriptome Sequencing and Analysis of HD11 Cells Infected by Avian Infectious Bronchitis Virus

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