猫杯状病毒单克隆抗体的制备

doi:10.11843/j.issn.0366-6964.2024.01.038

Abstract

Abstract: This study aimed to develop specific monoclonal antibodies for feline calicivirus (FCV) and provide materials for immunological diagnostic methods of FCV. In this study, BALB/c mice were immunized with FCV purified by sucrose gradient centrifugation and inactivated. The positive hybridoma cells were screened by an indirect ELISA method and characterized by a series of methods. One hybridoma 3B11 stably secreting monoclonal antibodies was successfully obtained. The antibody type of MAb 3B11 was identified as IgG1 for the heavy chain and κ chain for the light chain. MAb 3B11 specifically reacted with FCV-SH192. The mouse ascites MAb 3B11 showed high ELISA titers against five FCV strains; it had IFA and Western blot binding characteristics with five FCV strains. The MAb 3B11 developed in this study has good specificity, immunoreactivity and broad-spectrum recognition of FCV, which provides biological raw materials for the optimization of FCV diagnostic methods, screening of epidemic strains and basic research.

Key words: feline calicivirus, monoclonal antibodies, specificity, reactivity

CLC Number:

S852.659.6

WANG Ying, LI Jiakang, ZENG Yue, SHI Kaituo, WEI Liting, PENG Jiajia, LI Qiuyan, CAO Longlong, ZHOU Dengyuan. Development of Monoclonal Antibodies against Feline Calicivirus[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 401-405.

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Development of Monoclonal Antibodies against Feline Calicivirus

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