川乡黑猪、巴克夏猪、杜洛克猪肠道微生物多样性差异分析

doi:10.11843/j.issn.0366-6964.2026.01.013

Abstract

Abstract:

This study aimed to investigate the differences in intestinal microbial diversity among Chuanxiang Black pigs， Berkshire pigs， and Duroc pigs using 16S rRNA sequencing technology and bioinformatics analysis. When the body weight of the pigs reached 120 kg， Chuanxiang Black pigs， Berkshire pigs， and Duroc pigs were slaughtered， and rectal fecal samples were collected from each breed. The intestinal microbial diversity of the different pig breeds was then analyzed using 16S rRNA sequencing technology and bioinformatics approaches. The results demonstrated that the microbial diversity of Berkshire pigs was significantly lower than that of Chuanxiang Black pigs and Duroc pigs （P<0.05）， and the microbial community structures among the 3 pig breeds showed significant differences （P<0.05）. At the phylum level， the abundance of Firmicutes in Chuanxiang Black pigs was significantly lower than that in Berkshire pigs （P<0.05）， while the abundance of Bacteroidota in both Chuanxiang Black pigs and Duroc pigs was significantly higher than that in Berkshire pigs （P<0.05）. At the genus level， the abundance of Rikenellaceae RC9 gut group in Chuanxiang Black pigs and Duroc pigs was significantly higher than that in Berkshire pigs （P<0.05）. The abundances of Prevotella and Prevotellaceae NK3B31 group in Chuanxiang Black pigs were significantly higher than those in Duroc pigs and Berkshire pigs （P<0.05）， while the abundance of Clostridium sensu stricto 1 in both Duroc pigs and Berkshire pigs was significantly higher than that in Chuanxiang Black pigs （P<0.05）. LEfSe analysis showed that there were 14， 7， and 1 differential microorganisms in Chuanxiang Black pigs， Berkshire pigs， and Duroc pigs at the genus level， respectively. Functional prediction results showed that pathways related to amino acid metabolism were significantly enriched in the microbial community of Chuanxiang Black pigs， pathways related to pyruvate metabolism and others were significantly enriched in Berkshire pigs， and pathways related to protein transport and others were significantly enriched in Duroc pigs. In conclusion， there are significant differences in the composition and function of intestinal microbiota among Chuanxiang Black pigs， Berkshire pigs， and Duroc pigs. These differences may affect the host's nutritional metabolism， energy utilization， immune regulation， and other functions.

Key words: Chuanxiang black pigs, Berkshire pigs, Duroc pigs, 16S rRNA, microbial diversity

CLC Number:

S828.2

WANG Yan, GU Yiren, ZHANG Jinwei, YANG Xuemei, LIANG Yan, LIU Rui, TAO Xuan, YANG Yuekui, AN Rui, YANG Kun, ZHANG Xing, CHEN Xiaohui, GONG Jianjun. Analysis of Intestinal Microbial Diversity Differences among Chuanxiang Black Pigs， Berkshire Pigs and Duroc Pigs[J]. Acta Veterinaria et Zootechnica Sinica, 2026, 57(1): 146-154.

Figures/Tables 9

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References 31

[1]	冯思敏.高低肌内脂肪含量杜洛克猪遗传与肠道微生物组成比较研究［D］.武汉：华中农业大学，2023.
	FENG S M.Comparision of genetic and intestinal microbiota composition between Duroc pigs with high and low intramuscular fat content［D］.Wuhan：Huazhong Agricultural University，2023.（in Chinese）
[2]	王盼，王忠刚，吴卓穗，等.改善猪肉品质的研究进展［J］.广东畜牧兽医科技，2024，49（6）：50-56.
	WANG P，WANG Z G，WU Z S，et al.Research progress on improving pork quality［J］.Guangdong Journal of Animal and Veterinary Science，2024，496）：50-56.（in Chinese
[3]	陶璇，梁艳，应三成，等.川乡黑猪杂交试验研究［J］.中国畜牧杂志，2021，57（S1）：214-217.
	TAO X，LIANG Y，YING S C，et al.Study on crossbreeding experiments of Chuanxiang Black pigs［J］.Chinese Journal of Animal Science，2021，57（S1）：214-217.（in Chinese）
[4]	孟兴缘，陈科.国内首个自主知识产权生猪父本新品种育成［N］.科技日报，2021-12-29（006）.
	MENG X Y，CHEN K.China's first new pig sire breed with independent intellectual property rights has been developed ［N］.Science and Technology Daily，2021-12-29（006）.（in Chinese）
[5]	HUANG J Q，ZHANG W J，FAN R，et al.Conposition and functional diversity of fecal bacterial community of wild boar，comercial pig and domestic native pig as revealed by 16S rRNA gene sequencing［J］.Arch Microbiol，2020，202 （4）：843-857.
[6]	YANG L N，BIAN G R，SU Y，et al.Comparison of faecal microbial community of lantang，bama，erhualian，meishan，xiaomeishan，duroc，landrace，and yorkshire sows［J］.Asian-Australas J Anim Sci，2014，27（6）：898-906.
[7]	LI C，ZHAO X Y，ZHAO G S，et al.Comparative analysis of structural composition and function of intestinal microbiota between Chinese indigenous Laiwu pigs and commercial DLY pigs［J］.Vet Sci，2023，10 （8）：524.
[8]	YANG H，XIAO Y P，WANG J J，et al.Core gut microbiota in Jinhua pigs and its correlation with strain，farm and weaning age［J］.J Microbiol，2018，56（5）：346-355.
[9]	PAJARILLO E A，CHAE J P，BALOLONG M P，et al.Characterization of the fecal microbial communities of Duroc pigs using 16S rRNA gene pyrosequencing［J］.Asian-Australas J Anim Sci，2015，28（4）：584-591.
[10]	DIAO H，YAN H L，XIAO Y，et al.Intestinal microbiota could transfer host gut characteristics from pigs to mice［J］.BMC Microbiol，2016，16（1）：238.
[11]	XIAO Y P，KONG F L，XIANG Y，et al.Comparative biogeography of the gut microbiome between Jinhua and Landrace pigs［J］.Sci Rep，2018，8（1）：5985.
[12]	BERGAMASCHI M，TIEZZI F，HOWARD J，et al.Gut microbiome composition differences among breeds impact feed efficiency in swine［J］.Microbiome，2020，8（1）：110.
[13]	CHEN S F，ZHOU Y Q，CHEN Y R，et al.fastp：an ultra-fast all-in-one FASTQ preprocessor［J］.Bioinformatics，2018，34（17）：i884-i890.
[14]	MAGOC T，SALZBERG S L.FLASH：fast length adjustment of short reads to improve genome assemblies［J］.Bioinformatics，2011，27（21）：2957‐2963.
[15]	EDGAR R C.UPARSE：highly accurate OTU sequences from microbial amplicon reads［J］.Nat Methods，2013，10（10）：996‐998.
[16]	STACKEBRANDT E，GOEBEL B M.Taxonomic Note：A place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology［J］.Int J System Bacteriol，1994，44（4）：846-849.
[17]	WANG Q，GARRITY G M，TIEDJE J M，et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy［J］.Appl Environ Microbiol，2007，73（16）：5261‐5267.
[18]	杨雪梅，杨跃奎，曾凯，等.川乡黑猪新品种培育［J］.中国畜禽种业，2025，21（1）：49-56.
	YANG X M，YANG Y K，ZENG K，et al.Cultivation of Chuanxiang Black pig breed［J］.The Chinese Livestock and Poultry Breeding，2025，21（1）：49-56.（in Chinese）
[19]	ZHANG S H，ZHANG H，ZHANG C，et al.Composition and evolutionary characterization of the gut microbiota in pigs［J］.Int Microbiol，2024，27（4）：993-1008.
[20]	TURNBAUGH P J，BACKHED F，FULTON L，et al.Diet-induced obesity is linked to marked but reversible alterations in the mouse distal gut microbiome［J］.Cell Host Microbe，2008，3（4）：213-223.
[21]	FLINT H J，SCOTT K P，DUNCAN S H，et al.Microbial degradation of complex carbohydrates in the gut［J］.Gut Microbes，2012，3（4）：289-306.
[22]	梁艳，陶璇，杨雪梅，等.川藏黑猪S05系育肥性能研究［J］.黑龙江畜牧兽医，2014，（3）：61-62，65.
	LIANG Y，TAO X，YANG X M，et al.Study on the fattening performance of the S05 line of Sichuan-Tibet black swine［J］.Heilongjiang Animal Science and Veterinary Medicine，2014，（3）：61-62，65.（in Chinese）
[23]	YU S Q，LI L X，ZHAO H Y，et al.Characterization of the dynamic changes of ruminal microbiota colonizing citrus pomace waste during rumen incubation for volatile fatty acid production［J］.Microbiol Spectrum，2023，11（2）：10.1128.
[24]	FRAMPTON J，MURPHY K G，FROST G，et al.Short-chain fatty acids as potential regulators of skeletal muscle metabolism and function［J］.Nat Metab，2020，2（9）：840-848.
[25]	杨跃奎，龚建军，陶璇，等.川藏黑猪S04系生长发育及肉质性状选育进展研究［J］.中国畜牧兽医文摘，2014，30（9）：70-71.
	YANG Y K，GONG J J，TAO X，et al.Study on the breeding progress of growth development and meat quality traits of Chuanzang Black pig S04 line［J］.Chinese Journal of Animal Husbandry and Veterinary Medicine Abstracts，2014，30（9）：70-71.（in Chinese）
[26]	张洁，张力莉，徐晓锋.反刍动物瘤胃内普雷沃氏菌的研究进展［J］.中国饲料，2020，（7）：17-21.
	ZHANG J，ZHANG L L，XU X F.Research progress on prevotella in rumen of ruminants［J］.China Feed，2020，（7）：17-21.（in Chinese）
[27]	FAN P X，LIU P，SONG P X，et al.Moderate dietary protein restriction alters the composition of gut microbiota and improves ileal barrier function in adult pig model［J］.Sci Rep，2017，7：43412.
[28]	YANG W Y，LEE Y，LU H，et al.Analysis of gut microbiota and the effect of lauric acid against necrotic enteritis in Clostridium perfringens and Eimeria side-by-side challenge model［J］.PLoS One，2019，14（5）：e0205784.
[29]	MA X Y，YU M，LIU Z C，et al.Effect of amino acids and their derivatives on meat quality of finishing pigs［J］.J Food Sci Technol，2020，57（2）：404-412.
[30]	TIAN W C，YANG L，LIU Y S，et al.Resveratrol attenuates doxorubicin-induced cardiotoxicity in rats by up-regulation of vascular endothelial growth factor B［J］.J Nutr Biochem，2020，79：108132.
[31]	赵敏孟.胚蛋注射丙酮酸肌酸调控肉鸡能量代谢和肌肉发育的作用机理研究［D］.南京：南京农业大学，2017.
	ZHAO M M.Regulatory mechanism of in ovo feeding of creatine pyruvate on energy metabolism and muscle development of broiler chichens ［D］.Nanjing：Nanjing Agricultural University，2017.（in Chinese）

Analysis of Intestinal Microbial Diversity Differences among Chuanxiang Black Pigs， Berkshire Pigs and Duroc Pigs

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