猪源致病性格氏乳球菌的分离鉴定及毒力基因分析

doi:10.11843/j.issn.0366-6964.2025.07.032

Abstract

Abstract:

This study aimed to explore the biological characteristics, drug sensitivity and pathogenicity of Lactococcus garvieae from pig. Suspicious bacteria were isolated from the liver of diseased pig, and then were identified by Gram stained microscopy, matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), 16S rRNA gene sequence analysis and sequence analysis. The drug resistance and pathogenicity of isolated strains were explored through drug sensitivity testing and mice infection testing. Related virulence of isolated strain genes were amplified by PCR. Results were as follows: A positive strain of α-hemolytic was isolated from the liver of a diseased pig. MALDI-TOF MS, PCR amplification of 16S rRNA gene and phylogenetic tree analysis results showed that the isolated strain was identified as Lactococcus garvieae.The infection test of mice showed that the isolated strain had strong lethality. It can cause swelling of the liver and spleen, severe congestion of the intestines, abdominal cavity, and chest cavity in mice. The drug sensitivity test results showed that the isolated strains were resistant to β-lactam drugs such as penicillin G and ampicillin, as well as erythromycin and lincomycin, and sensitive to other antibiotics. The results of virulence gene testing showed that the isolated strain contains adhesion related genes adhC1, psaA, eno, lp1, hemolysis related genes hly1, hly2, hly3, capsule gene cluster formation related genes cgcC, polysaccharide related genes epsA, epsB, epsC, epsD, epsL, and other virulence genes nadho, sod, pgm, chp. The Lactococcus garvieae was isolated in this experiment has strong pathogenicity, and its potential risks cannot be ignored as it belongs to zoonotic pathogens. This study provides data reference for the prevention and treatment of clinical infections caused by Lactococcus garvieae.

Key words: Lactococcus garvieae, pig, 16S rRNA, drug susceptibility test, virulence gene

CLC Number:

S858.285.1

SHU Jingchao, ZHANG Han, PENG Zhifeng, QIAO Hongxing. Isolation, Identification and Virulence Gene Analysis of Porcine Pathogenic Lactococcus garvieae[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3399-3407.

Figures/Tables 6

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Table 1

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References 26

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抗菌药物Antimicrobials	抑菌圈直径折点(D)/mm Zone diameter breakpoints			抑菌圈直径/mmZone diameter	敏感性Sensitivity
抗菌药物Antimicrobials	R	I	S	抑菌圈直径/mmZone diameter	敏感性Sensitivity
大观霉素Spectinomycin	≤14	14＜D＜18	≥18	19	S
庆大霉素Gentamicin	≤12	12＜D＜15	≥15	25	S
四环素Tetracycline	≤4	4＜D＜19	≥19	22	S
多西环素Doxycycline	≤9	9＜D＜13	≥13	27	S
头孢唑林Cefuroxime	≤19	19＜D＜23	≥23	31	S
头孢他啶Ceftazidime	≤17	17＜D＜21	≥21	30	S
头孢吡肟Cefepime	≤18	18＜D＜25	≥25	33	S
氧氟沙星Ofloxacin	≤13	13＜D＜17	≥17	22	S
环丙沙星Ciprofloxacin	≤15	15＜D＜21	≥21	29	S
恩诺沙星Enrofloxacin	≤15	15＜D＜21	≥21	22	S
阿米卡星Amikacin	≤14	14＜D＜17	≥17	18	S
加替沙星Gatifloxacin	≤14	14＜D＜18	≥18	23	S
青霉素G Penicillin G	≤28	-	≥29	0	R
氨苄西林Ampicillin	≤13	13＜D＜17	≥17	0	R
红霉素Erythromycin	≤13	13＜D＜23	≥23	9	R
氯霉素Chloramphenicol	≤12	12＜D＜18	≥18	22	S
新霉素Neomycin	≤16	16＜D＜22	≥22	30	S
阿奇霉素Azithromycin	≤13	13＜D＜18	≥18	15	I
复方新诺明Bactrim	≤10	10＜D＜16	≥16	37	S
磺胺异恶唑Sulfisoxazole	≤12	12＜D＜17	≥17	21	S
亚胺培南Imipenem	≤19	19＜D＜23	≥23	15	R
美罗培南Meropenem	≤19	19＜D＜23	≥23	24	S
林可霉素Lincomycin	≤9	9＜D＜15	≥15	0	R
多黏菌素B Polymyxin B	≤8	8＜D＜12	≥12	25	S

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Isolation, Identification and Virulence Gene Analysis of Porcine Pathogenic Lactococcus garvieae

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