新疆地区3株驴源马链球菌马亚种新SeM基因型的鉴定与进化分析

doi:10.11843/j.issn.0366-6964.2022.01.023

Abstract

Abstract: Three isolates of Streptococcus subsp. equi HTP133, HTP123 and HTP232 were obtained from a donkey farm in Xinjiang. To identify the genotypingand understand the biological and epidemic characteristics of these three isolates,the biochemical characteristics analysis,drug sensitivity test and 16S rRNA sequence comparison analysis and phylogenetic tree construction were performed. The SeM alleles were amplified by PCR and sequenced for genotyping. The results showed that these three isolates were Streptococcus equi subsp.equi. These three isolates were sensitive to 18 drugs, including amoxicillin, ampicillin and gentamicin etc, whereas they are resistant to penicillin and cefuroxime. Form the 16S rRNA gene analysis and the phylogentic tree, we found that these isolated strains belonged to group I and there is an obvious variation in their variable regions of V1-V5. The homology of these three isolates are closely related with S. equi JQ726493. In addition,two new SeM genotypes, SeM 210 (HTP232) and SeM 211 (HTP133, HTP123) were identified by SeM allele analysis and they were closely related to SeM 136, SeM 138, SeM 142 and SeM 146 genotype.This work enriches the molecular biological foundation of prevalence and transmission characteristics of donkey S. equi in China and provides the theoreitical basis for the prevention and control of donkey strangles as well.

Key words: donkey Streptococcus equi subsp. equi, 16S rRNA analysis, SeM genotyping, drug sensitivity

CLC Number:

S852.611

DU Yu, PU Xiaofeng, CHEN Xiaomeng, Lü Fenfen, WANG Li, ZHANG Zehua, ZHANG Baojiang, SU Yan. Identification and Evolutionary Analysis of Three Novel SeM Genotypes of Donkey Streptococcus equi subsp. equi Isolates in Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 231-240.

References 24

[1]	刘建柱, 谢宁, 刘永夏. 马腺疫的研究进展[J]. 兽医导刊, 2018(5):20-21.LIU J Z, XIE N, LIU Y X. Advances in the study of horse adenosis[J]. Veterinary Orientation, 2018(5):20-21. (in Chinese)
[2]	朱曼玲, 齐鹏飞, 黄迪海, 等. 驴源马腺疫链球菌的分离与鉴定[J]. 中国动物检疫, 2020, 37(1):83-86.ZHU M L, QI P F, HUANG D H, et al. Isolation and identification of donkey-derived Streptococcus equinus[J]. China Animal Health Inspection, 2020, 37(1):83-86. (in Chinese)
[3]	孟庆刚. 马腺疫的防治[J]. 中国畜禽种业, 2020, 16(6):73.MENG Q G. Control of horse adenosis[J]. The Chinese Livestock and Poultry Breeding, 2020, 16(6):73. (in Chinese)
[4]	DURHAM A E, HALL Y S, KULP L, et al. A study of the environmental survival of Streptococcus equi subspecies equi[J]. Equine Vet J, 2018, 50(6):861-864.
[5]	KELLY C, BUGG M, ROBINSON C, et al. Sequence variation of the SeM gene of Streptococcus equi allows discrimination of the source of strangles outbreaks[J]. J Clin Microbiol, 2006, 44(2):480-486.
[6]	ANZAI T, KUWAMOTO Y, WADA R, et al. Variation in the N-terminal region of an M-like protein of Streptococcus equi and evaluation of its potential as a tool in epidemiologic studies[J]. Am J Vet Res, 2005, 66(12):2167-2171.
[7]	VAN BELKUM A, TASSIOS P T, DIJKSHOORN L, et al. Guidelines for the validation and application of typing methods for use in bacterial epidemiology[J]. Clin Microbiol Infect, 2007, 13(3):1-46.
[8]	IVENS P A S, MATTHEWS D, WEBB K, et al. Molecular characterisation of ‘strangles’ outbreaks in the UK:the use of M-protein typing of Streptococcus equi ssp. equi[J]. Equine Vet J, 2011, 43(3):359-364.
[9]	DONG J, GAO N, WALLER A S, et al. An outbreak of strangles associated with a novel genotype of Streptococcus equi subspecies equi in donkeys in China during 2018[J]. Equine Vet J, 2019, 51(6):743-748.
[10]	刘延麟. 驴源马链球菌马亚种新基因型感染的流行病学与规模化驴场腺疫的临床防治研究[D]. 长春:吉林大学, 2019.LIU Y L. Epidemiological investigation for the infection of novel genotypes of Streptococcus equi subspecies equi isolated from donkeys and prevention and treatment for strangles in intensive donkey farms[D]. Changchun:Jilin University, 2019. (in Chinese)
[11]	王彩蝶, 苏艳, 波拉提, 等. 新疆4县马腺疫链球菌血清抗体检测与分析[J]. 动物医学进展, 2015, 36(5):125-127.WANG C D, SU Y, BO L T, et al. Detection and analysis of serum antibodies aganist Streptococcus equi subspecies equi in four counties of Xinjiang[J]. Progress in Veterinary Medicine, 2015, 36(5):125-127. (in Chinese)
[12]	袁贝, 李海, 马陈, 等. 新疆伊犁地区马鼻肺炎、马腺疫、马流产沙门菌病的血清学调查[J]. 中国预防兽医学报, 2017, 39(7):540-543.YUAN B, LI H, MA C, et al. Serum epidemiology investigation of Equine rhinopneumonitis, Strangles, Salmonella abortus equi in Yili of Xinjiang[J]. Chinese Journal of Preventive Veterinary Medicine, 2017, 39(7):540-543. (in Chinese)
[13]	冯凯, 王浩, 周婷婷, 等. 马源马链球菌兽疫亚种新疆株的分离鉴定及遗传特性分析[J]. 中国畜牧兽医, 2019, 46(1):231-238.FENG K, WANG H, ZHOU T T, et al. Isolation, identification and genetic characterization analysis of Streptococcus equi subsp. zooepidemicus from Horse in Xinjiang[J]. Chinese Animal Husbandry & Veterinary Medicine, 2019, 46(1):231-238. (in Chinese)
[14]	WALLER A S, ROBINSON C. Streptococcus zooepidemicus and Streptococcus equi evolution:the role of CRISPRs[J]. Biochem Soc Trans, 2013, 41(6):1437-1443.
[15]	苏战强, 孙雪, 翟少华, 等. 新疆一起驴腺疫的诊断与治疗[J]. 中国动物检疫, 2019, 36(7):89-94.SU Z Q, SUN X, ZHAI S H, et al. Diagnosis and treatment on a case of donkey strangles in Xinjiang[J]. China Animal Health Inspection, 2019, 36(7):89-94. (in Chinese)
[16]	ABDULMAWJOOD A, LÄMMLER C H. Determination of intraspecies variations of the V2 region of the 16S rRNA gene of Streptococcus equi subsp. zooepidemicus[J]. Res Vet Sci, 2000, 68(1):33-39.
[17]	MEEHAN M, LEWIS M J, BYME C, et al. Localization of the equine IgG-binding domain in the fibrinogen-binding protein (FgBP) of Streptococcus equi subsp. equi[J]. Microbiology, 2009, 155(8):2583-2592.
[18]	LIBARDONI F, VIELMO A, FARIAS L, et al. Diversity of seM in Streptococcus equi subsp. equi isolated from strangles outbreaks[J]. Vet Microbiol, 2013, 162(2-4):663-669.
[19]	吕芬芬, 马晓慧, 汪丽, 等. 马链球菌马亚种3种抗原蛋白对小鼠免疫的免疫原性分析[J]. 畜牧兽医学报, 2021, 52(3): 752-762.LÜ F F, MA X H, WANG L, et al. Immunogenicity analysis of three different antigen proteins of Streptococcus equi in mice[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(3): 752-762. (in Chinese)
[20]	TIMONEY J F, WALKER J, ZHOU M, et al. Cloning and sequence analysis of a protective M-like protein gene from Streptococcus equi subsp. zooepidemicus[J]. Infect Immun, 1995, 63(4):1440-1445.
[21]	TARTOR Y H, EL-NAENAEEY E Y, GHARIEB N M, et al. Novel Streptococcus equi strains causing strangles outbreaks in Arabian horses in Egypt[J]. Transbound Emerg Dis, 2020, 67(6):2455-2466.
[22]	刘云涛, 王彩蝶, 夏利宁, 等. 马链球菌马亚种新疆株SeM基因的克隆及序列差异分析[J]. 西北农业学报, 2015, 24(3):14-19.LIU Y T, WANG C D, XIA L N, et al. Cloning and sequence difference analysis of the SeM gene of Streptococcus equi subsp. equi isolated in Xinjiang[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2015, 24(3):14-19. (in Chinese)
[23]	王雨朦. 马链球菌马亚种的分离鉴定、致病性检测和抗菌药物筛选[D]. 乌鲁木齐:新疆农业大学, 2017.WANG Y M. Isolation, identification, pathogenicity detection and screening antibacterial drugs of Streptococcus equi subspecies equi[D]. Urumchi:Xinjiang Agricultural University, 2017. (in Chinese)
[24]	周婷婷, 冯凯, 汪丽, 等. 马链球菌马亚种新疆分离株的分离及耐药性和进化分析[J]. 中国兽医科学, 2019, 49(6):738-745.ZHOU T T, FENG K, WANG L, et al. Isolation, drug resistance and phylogenetic analysis of Streptococcus equi subsp, equi from horse in Xinjiang[J]. Chinese Veterinary Science, 2019, 49(6):738-745. (in Chinese)

Identification and Evolutionary Analysis of Three Novel SeM Genotypes of Donkey Streptococcus equi subsp. equi Isolates in Xinjiang

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 24

Related Articles 5

Recommended Articles

Metrics

Comments

[1]	TIAN Qiufeng, ZHANG Hong, YIN Junyi, SHI Tongrui, ZHANG Jun, LIU Qiujin, WANG Yan, QIN Pingwei, WANG Huan, BAI Changsheng, CHEN Nannan, ZHU Qinghe, MIAO Yan. Isolation and Identification of a Strain Acinetobacter johnsonii from Dairy Cow [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 324-328.
[2]	WANG Miao, ZHANG Xuejia, DU Wannian, YU Xiujian, LIU Boxing, WU Tonglei, SHI Qiumei, ZHANG Zhiqiang. Isolation, Identification and Drug Sensitivity Test of a Strain of Aeromonas veronii from Chinemys reevesii [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2528-2535.
[3]	WEN Fengqin, XIANG Haitao, HAO Baocheng, XING Xiaoyong, BAO Shijun, HU Yonghao. Isolation and Identification of Corynebacterium Xerosis Isolated from Yak Liver [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(1): 227-232.
[4]	HUANGFU He-ping, DU Zhen-long, DONG Qing, WANG Hong-kui, SUN Yan-ting, GUO Hong-wei, JI Xiang-bo, JIA Han-xiao, YU Meng-wei, BU Ya-ge. Isolation,Identification and Phylogenetic Analysis of Gallibacterium anatis from Silky Population in Henan Province, China [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2451-2460.
[5]	MA Xiao-ping, XIANG Qi, WANG Cheng-dong, CAO San-jie, LING Shan-shan, GU Yu. Isolation, Identification and Drug Resisitance Analysis of Yeast from Hair of Ailuropoda melanoleuca [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(12): 2383-2391.