归芪益母复方制剂对产后奶牛瘤胃微生物和短链脂肪酸的调节

doi:10.11843/j.issn.0366-6964.2022.12.031

Abstract

Abstract: This experiment aims to study the regulatory effects of Guiqiyimu compound preparation on rumen microbes and short-chain fatty acids in postpartum dairy cows. Nineteen healthy parturient Holstein cows were selected and randomly assigned to 2 groups with 10 cows in control group and 9 in experimental group. The dairy cows in experimental group were given 500 mL Guiqiyimu compound preparation everyday after delivery for 6 consecutive days, whereas the cows in the control group were given an equal volume of drinking water. The rumen fluid of each cow was collected before feeding in the morning at 7th day after delivery, 16S rRNA high-throughput sequencing and GC-MS/MS targeted metabolomics technology were used to determine the abundance of the rumen flora and the content of short-chain fatty acids in the two groups of dairy cows. The results of 16S rRNA sequencing exhibited that the abundances of the Proteobacteria, Escherichia-Shigella and Bacteroides were extremely significantly higher (P<0.01), Lactobacillus and Subdoligranulum were significantly higher (P<0.05) in experimental group than those of the control group. Correspondingly, the concentration of propionic acid (P<0.05) and isobutyric acid (P<0.01) in experimental group increased significantly. This study reveals the health care mechanism of Guiqiyimu formulae on postpartum dairy cows from the changes of rumen microbial flora and short-chain fatty acids.

Key words: Guiqiyimu compound preparation, rumen microbes, short-chain fatty acids, 16S rRNA high-throughput sequencing, GC-MS/MS targeted metabolomics

CLC Number:

S853.9

REN Man, LIU Xin, TANG Yulin, ZHANG Ruixue, QIN Junjie, ZHU Hao, GUO Yansheng. Regulation of Guiqiyimu Compound Preparation on Rumen Microbes and Short-chain Fatty Acids in Postpartum Dairy Cows[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12): 4461-4469.

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Regulation of Guiqiyimu Compound Preparation on Rumen Microbes and Short-chain Fatty Acids in Postpartum Dairy Cows

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