新疆野兔DNA条形码筛选

doi:10.11843/j.issn.0366-6964.2020.02.008

Abstract

Abstract: The purpose of this study was to screen the optimal DNA barcode for the rapid identification of hares in Xinjiang. A total of 4 hare breeds including 40 samples were collected, CO1, ND4, 16S rRNA and ITS2 genes were selected as candidate sequences. After PCR amplification and sequencing, the identification ability of candidate sequences at species level were analyzed and compared. The results showed that ITS2 fragments was first eliminated because no PCR amplification product was acquired for sequencing after repeated experiments. Compared with 16S rRNA, CO1 and ND4 showed stronger variation levels in sequence characteristics, variation characteristic parameters, and rank sum test results, their genetic frequency distribution map had wider gap, and the identification results of the systematic clustering tree had higher accuracy. Further, the characteristics of interspecies variation, interspecies rank sum test results and genetic frequency distribution map all indicated that ND4 was slightly better than CO1. By comprehensive analysis, the optimal DNA barcode obtained in this study was ND4, while CO1 could serve as the alternate one. These results would provide reference for the study of distribution and classification of hares in Xinjiang and their protection and utilization.

Key words: Lepus, DNA barcode, CO1, ND4, 16S rRNA, ITS2

CLC Number:

S829.12

ZHANG Yucong, ZENG Wanqin, XU Pan, Gulmire·ALIM, SHAN Wenjuan. The Screening of DNA Barcode for Hares in Xinjiang[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(2): 270-278.

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The Screening of DNA Barcode for Hares in Xinjiang

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