Table 1

Primer sequences of qRT-PCR"

Table 2

Primer sequences of qRT-PCR"

Fig. 1

The mRNA expression levels of IL-1β, TNF-α, and IL-6 in cells infected with the recombinant strain at 6, 12, 24, and 48 hours post-infection mRNA expression levels of cytokines in Ms-pMV361, Ms-pMV361-Rv3435c infected RAW264.7 cells at 6, 12, 24, and 48 h(*. P < 0.05; **. P < 0.01; ***. P < 0.001; ns. P>0.05). A. IL-1β; B. TNF-α; C. IL-6"

Table 3

Transcriptome sequencing data under recombinant Mycobacterium smegmatis infection"

Fig. 2

Homogeneity of gene coverage"

Fig. 3

Transcriptome sample relationship analysis A. Violin diagram of gene expression in the sample; B. Inter-sample PCA analysis plot"

Table 4

Statistics of differential expression results"

Fig. 4

Statistical results of differential expression A. Volcano diagram of expression differences[The horizontal axis is log2 (Fold change), and the vertical axis is-log10 (P-value). The two vertical dashed lines in the figure represent the threshold for expressing the difference factor; The horizontal dashed line represents the significance level threshold. The color represents genes that are red up (upregulated), blue down (downregulated), or gray none (non significantly differentially expressed), indicating the top 10 genes that are upregulated or downregulated (ranked by significance); B. Differential gene Venn diagram"

Table 5

The results of the top 10 GO enrichment analyses in the differentially igeneally speaking"

Fig. 5

GO enrichment analysis of differentially differentiated genes The ordinate is GO Term, and the abscissa is-lg(P-value)of GO Term enrichment"

Table 6

KEGG pathway categories of differentially expressed genes"

Fig. 6

KEGG analysis results of differentially differentiated genes A. KEGG pathway histogram; B. KEGG factor chart"

Fig. 7

Candidate targets were identified by protein-protein interaction (PPI)analysis A. STRING protein-protein interaction network; B. PPI Core Subnetwork I.; C.Visualization of differences in Cytoscape protein-protein interaction network; D. PPI Core Subnetwork Ⅱ"

Fig. 8

Verification of transcriptome results by RT-qPCR A. FPKM value of the up-regulation factors; B. Relative expression of up-regulated factors; C. FPKM value of the down-regulation factors; D. Relative expression of down-regulated factors. *.P < 0.05; **. P < 0.01; ***. P < 0.001; ns. P>0.05"

Fig. 9

SPP1 as a potential target for the function of Rv3435c A. Spp1 mRNA expression of recombinant strain 12 hours after infection; B. SPP1 secretion of recombinant strain 12 hours after infection; C. SPP1 protein level 12 h after infection by the recombinant strain.*.P < 0.05; **. P < 0.01; ***. P < 0.001; ns. P>0.05"