SIRT1基因激活后鸡巨噬细胞转录组分析

doi:10.11843/j.issn.0366-6964.2025.06.012

Abstract

Abstract:

This study aimed to activate avian macrophages using a selective SIRT1 activator and identify relevant target genes and signaling pathways through transcriptomic analysis. Chicken macrophages were treated with a selective SIRT1 activator, SRT1720 HCl, at a concentration of 1 μmol·L^-1. The experiment was divided into a control group (treated with DMSO) and an experimental group (treated with SRT1720 HCl), with 5 biological replicates per group. After 24 hours of treatment, total cellular RNA was extracted and reverse transcribed. High-throughput sequencing was then performed using the Illumina Novaseq X Plus platform, and subsequent bioinformatics analyses were conducted. Differentially expressed genes (DEGs) were identified using thresholds of |log₂ FC|>1 and an adjusted P < 0.05. A total of 1 183 DEGs were detected (P < 0.05), of which 781 were upregulated and 402 were downregulated, including key genes such as CD14, TLR7, IL10RA, NFKBIA, and JUN. Five genes selected from the DEGs were validated by real-time quantitative PCR, and the results were consistent with the transcriptomic data. Gene Ontology (GO) enrichment analysis revealed significant enriched terms associated with metabolic processes, immune system processes, biological adhesion, and catalytic activity (P < 0.05). Additionally, Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis results showed significant enriched pathways including the MAPK signaling pathway, Toll-like receptor signaling pathway, and NOD-like receptor signaling pathway. Notably, enrichment of the Salmonella infection pathway was observed, suggesting that activation of SIRT1 in avian macrophages might influence Salmonella infection. Protein-protein interaction network analysis further indicated that SIRT1 was closely related to genes such as NFKBIA and JUN. The study utilized transcriptome analysis of chicken macrophages after SIRT1 gene activation to screen for immune-related genes and important pathways, provides a research foundation for exploring the role of SIRT1 in avian immunity.

Key words: SIRT1, avian macrophage, transcriptome

CLC Number:

S831.2

LIU Sha, SU Meng, GAO Qianmei, SONG Danli, ZHAO Guiping, LI Jianhui, LI Qinghe. Transcriptome Analysis of Chicken Macrophages after SIRT1 Activated[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2661-2671.

Figures/Tables 5

Table 1

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基因Gene	上游引物(5′→3′)Forward	下游引物(5′→3′)Reverse
β-actin	GAGAAATTGTGCGTGACATCA	CCTGAACCTCTCATTGCCA
CD14	ACCCGACACTTGACCCTGTTG	TCTGCCTTAGGGGAAAAGGGC
NFKBIA	TGGGAGAACAGCACTACACT	GCCCTGGTAGGTCACTTTG
TLR7	GCACACCGGAAAATGGTACA	TTGGGAAACCAACGTCCTGAT
JUN	AACTCCGCACCCAACTAC	GGTACAGTCTGAGGCTCTTCT
IL10RA	TGGGCATCTTCGACACTGAC	GAGTTGGTTTGCACCGTGAG

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Transcriptome Analysis of Chicken Macrophages after SIRT1 Activated

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