Abstract:

Parasitic diseases in livestock and poultry are one of the major threats to agriculture and public health worldwide. These diseases not only lead to reduced productivity in animals but can also be transmitted to humans through the food chain, posing serious public health risks. Therefore, accurate diagnosis of parasitic infections in livestock and poultry is of paramount importance, as it enables timely identification and control of diseases, minimizes economic losses, and protects human health. However, traditional diagnostic methods often suffer from limitations in sensitivity and specificity, restricting their application in early diagnosis and disease surveillance. In recent years, advances in nanotechnology and CRISPR gene-editing technology have provided new tools and approaches for the precise diagnosis of parasitic diseases in animals. Nanotechnology, particularly the use of nanomaterials with high surface area and unique optical and electrical properties, has shown great potential in biosensing and molecular diagnostics. CRISPR-based diagnostic technology, known for its accuracy and efficiency, has emerged as a powerful tool in gene function research and disease detection. The integration of these two technologies can lead to the development of more sensitive, specific, and rapid diagnostic methods to deal with the challenges posed by parasitic infections in livestock and poultry. This review will explore the application of nanotechnology and CRISPR-based diagnostic techniques in the accurate diagnosis of animal parasitic diseases. By investigating their potential and prospects, this work aims to support early diagnosis, vaccine development, and effective control strategies of livestock parasitic diseases, ultimately contributing to animal health and improved agricultural productivity.

Key words: parasitic diseases, precise diagnosis, nanotechnology, CRISPR/Cas