引导编辑技术的研究进展及应用

doi:10.11843/j.issn.0366-6964.2024.04.001

Abstract

Abstract: The prime editing system based on CRISPR/Cas9 is a novel gene editing technology, which enables all 12 base-to-base conversions or transition, precise small insertion and deletion without requiring double-strand breaks or exogenous donor DNA templates. This review summarizes the development of gene editing approaches, the principle and characteristics of prime editing technique, as well as applications in animal and plant breeding and human gene therapy. It provides a reference for scientists to get insight into prime editing system and further use it in basic research and animal or plant breeding.

Key words: CRISPR/Cas9, prime editing(PE), pegRNA, application research

CLC Number:

S813.1

QIU Meiyu, ZHANG Xuemei, ZHANG Ning, LIU Mingjun. Approach and Application of Prime Editing System[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1345-1355.

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Approach and Application of Prime Editing System

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