赖氨酸乙酰化修饰在鸡精液冷冻中的保护作用及机制研究进展

doi:10.11843/j.issn.0366-6964.2025.07.003

Abstract

Abstract:

China has a rich diversity of chicken breeds with 140 native breeds, which harbor numerous excellent genetic traits. The conservation, breeding, and utilization of chicken genetic resources are of significant importance for promoting the independent development of national chicken breeding industry. The ultra-low temperature cryopreservation of semen is one of the most practical techniques for the long-term preservation and efficient utilization of livestock and poultry genetic resources. The semen cryopreservation technology for livestock, particularly for cattle, is relatively well-developed, but the technology was limited in chicken due to the long tails and small heads of spermatozoa, which result in poor freeze resistance. The poor fertility and sustained fertilization ability of chicken frozen sperm limited the application of semen cryopreservation technique in conservation and breeding. Post-translational modification of proteins was an important regulatory pathway through which cells respond to internal and external environmental stimuli. In mature sperm, both transcription and translation activities were largely halted, lysine acetylation modification of proteins might play crucial roles during the cryopreservation of chicken semen. These modifications could be essential for maintaining sperm structural integrity, mitochondrial function stability, and DNA integrity. However, the specific mechanisms underlying these effects remain unclear. This review aimed to provide a reference for investigating the role of lysine acetylation in the cryopreservation of chicken semen and establishing novel cryopreservation methods, and to offer theoretical insights and technical support for the efficient conservation of poultry genetic resources.

Key words: chicken, semen cryopreservation, sperm, lysine acetylation modification, germplasm resource

CLC Number:

S831.3

ZONG Yunhe, YANG Yuze, SUN Yanyan, CHEN Jilan, LI Yunlei. Research Advances in the Investigation of the Protective Role of Lysine Acetylation in Chicken Semen Cryopreservation and Its Mechanism[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(7): 3071-3079.

Figures/Tables 4

Fig. 1

Table 1

Table 2

Fig. 2

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家族 Family	酶(别名) Enzyme (alias)	特异性抑制剂 Specific inhibitor	睾丸 Testis	精子 Sperm	精浆 Seminal plasma
GNAT	HAT1 (KAT1)	CPTH6	-	√	-
	GCN5 (KAT2A)	CPTH2 (C₁₄H₁₄ClN₃S)	√	-	-
MYST	Tip60 (KAT5)	TH1834 (C₃₃H₄₀N₆O₃)	-	√	-
	MORF (KAT6B)	-	-	√	√
其他Other	TAF1(KAT4)	CeMMEC13(C₁₉H₁₆N₂O₄)	√	-	-

家族 Family	酶 Enzyme	通用抑制剂 Universal inhibitor	特异性抑制剂 Specific inhibitor	睾丸 Testis	精子 Sperm	精浆 Seminal plasma
HDAC Zn²⁺依赖型 Zn²⁺-dependent	HDAC1	TSA (C₁₇H₂₂N₂O₃)	HDAC1-IN-4 (C₂₁H₂₄BrClN₆O₂)	√	-	-
	HDAC2		HDAC2-IN-1 (C₂₂H₂₃ClN₄OS)	√	-	-
	HDAC8		HDAC8-IN-3 (C₁₈H₁₂N₄O₃S₂)	-	√	-
	HDAC7		-	√	-	-
	HDAC10		HDAC10-IN-2 (C₁₉H₂₂N₂O₂)	-	√	-
	HDAC11		FT895 (C₁₆H₁₅F₃N₄O₂)	√	-	-
SIRT NAD+依赖型 NAD⁺-dependent	SIRT2	NAM(C₆H₆N₂O)	SirReal2 (C₂₂H₂₀N₄OS₂)	√	√	-
	SIRT3		3-TYP (C₇H₆N₄)	√	√	-
	SIRT5		NRD167 (C34H46N6O6S)	√	√	-

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Research Advances in the Investigation of the Protective Role of Lysine Acetylation in Chicken Semen Cryopreservation and Its Mechanism

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