G3BP1基因在鸡肌内前脂肪细胞增殖与分化中的作用及其分子标记鉴定

doi:10.11843/j.issn.0366-6964.2025.01.015

Abstract

Abstract:

The study aimed to identify SNP sites of the candidate gene G3BP1 that influence intramuscular fat deposition using a population of 721 Gushi F2 chickens, to develop molecular markers for meat quality traits, and to further investigate its function using intramuscular preadipocytes from Gushi chickens. First, the study identified SNPs in G3BP1 gene and found 3 mutation sites in strong linkage disequilibrium, especially the different genotypes of the 13588667G>A site were significantly associated with the meat quality traits such as sebum, sebum rate and intramuscular fat in the F2 resource group of Gushi chicken (P < 0.05). Therefore, the 13588667G>A site of the G3BP1 gene could be used as an important molecular marker affecting intramuscular fat deposition. Additionally, qRT-PCR, CCK-8, flow cytometry, triglyceride content determination and Oil Red O staining were used to evaluate the effect of G3BP1 gene on the proliferation and differentiation of intramuscular preadipocytes in Gushi chickens. The results showed that after overexpression of G3BP1, the expression levels of proliferation marker genes CDK1, CCNB2, PCNA and CCND1 in intramuscular preadipocytes were significantly increased (P < 0.001), and the cell cycle process was significantly promoted. G3BP1 overexpression also significantly upregulated the expression of the differentiation marker gene LPL (P < 0.05) and dramatically increased the expression of CEBPA (P < 0.001), with a significant increase in lipid droplet formation and triglyceride content in intramuscular preadipocytes (P < 0.01). This study clarifies the role of G3BP1 in promoting the proliferation and adipogenesis of intramuscular preadipocytes and identifies a molecular marker affecting intramuscular fat deposition, providing valuable theoretical and practical insights for breeding high-quality meat chickens in China.

Key words: Gushi chicken, intramuscular fat, G3BP1, molecular marker

CLC Number:

S831.2

LI Yuanfang, WU Ran, LI Shuaihao, WEI Qianran, WANG Yadong, WANG Dandan, LI Zhi, LI Guoxi, LIU Qiaoming. The Role of G3BP1 in the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes and Identification of Its Molecular Markers[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(1): 159-167.

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References 40

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基因Gene	引物序列(5′→3′)Primer sequence	片段长度/bp Length	退火温度/℃ Tm
G3BP1	F: CTCCAAATCCTGGCTTCT R: GAGGGTCTGCTGTTCTTCAT	563	60
CCNB2	F: CCTCTTCCACTTCACTTCT R: CTTTGTACCCCACTTATCA	195	60
CCND1	F: CAGAAGTGCGAAGAGGAAGT R: CTGATGGAGTTGTCGGTGTA	188	60
PCNA	F: AGCACCAAATCAGGAAAAG R: GCACAGGAGATGACAACAG	177	60
CDK1	F: TAATAGATGACAAAGGGGT R: GAGTGGAATACAGAGCAGA	147	60
LPL	F: TCCGATCCCGAAGCTGAGA R: ACATTCCTGTCACCGTCCAC	167	60
FABP4	F: ATATGAAAGAGCTGGGTGTGGG R: CAGTGTGCCATTGTCTAGGGT	218	60
FABP5	F: TGGCCATCGACGCGTTTTTA R: TCTGGTTTCGCCATGCTTCC	121	60
FASN	F: ACAACAGCCAGCTTGGAATG R: CAGGGGCTTTACCACACCAT	152	60
PPARγ	F: GTGCAATCAAAATGGAGCC R: CTTACAACCTTCACATGCAT	170	60
CEBPA	F: TTCTACGAGGTCGATTCCCG R: AGCCTCTCTGTAGCCGTAG	96	60
GAPDH	F: GAACATCATCCCAGCGTCCA R: CGGCAGGTCAGGTCAACAAC	132	60

突变位点 SNP site	数量 Number	基因型频率 Genotype frequency			等位基因频率 Allele frequency		杂合度 Heterozygosity
突变位点 SNP site	数量 Number	II	ID	DD	I	D	杂合度 Heterozygosity
13588667G>A	721	0.681	0.288	0.030	0.836	0.164	0.274
13588768C>A	721	0.575	0.362	0.062	0.768	0.232	0.356
13588930C>T	721	0.241	0.447	0.312	0.438	0.562	0.492

相关指标 Relevant metrics	基因型 Genotype			P值 P-value
相关指标 Relevant metrics	GG	GA	AA	P值 P-value
皮脂/g Sebum	7.316±0.522^b	9.667±0.799^a	5.898±2.454^ab	0.033
皮脂率/% Sebum rate	0.801±0.055^b	1.038±0.085^a	0.649±0.264^b	0.045
肌间脂肪宽/cm Intermuscular fat width	0.729±0.012	0.737±0.019	0.720±0.058	0.929
皮下脂肪厚/cm Subcutaneous fat thickness	0.457±0.008	0.464±0.012	0.454±0.035	0.862
鲜样脂肪含量/% Fat content in fresh samples	0.719±0.013	0.705±0.019	0.711±0.060	0.833
绝干脂肪含量/% Fat content in absolute dry samples	2.747±0.047	2.705±0.072	2.746±0.222	0.886
肌内脂肪含量/% Intramuscular fat content	2.681±0.031^a	2.437±0.047^b	2.244±0.145^b	＜0.001

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The Role of G3BP1 in the Proliferation and Differentiation of Chicken Intramuscular Preadipocytes and Identification of Its Molecular Markers

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