Acta Veterinaria et Zootechnica Sinica ›› 2025, Vol. 56 ›› Issue (3): 1159-1169.doi: 10.11843/j.issn.0366-6964.2025.03.017

• Animal Genetics and Breeding • Previous Articles     Next Articles

The Effect of Riboflavin Supplementation in Embryonic Eggs on the Development of Skeletal Muscle of Chickens

LI Yuanfang1,2(), ZHANG Hongyuan3,4, LI Hongtai3,4, LI Zhi1,2, WEI Qianran1,2, WANG Yadong1,2, LI Guoxi3,4,*(), WANG Dandan5,*(), LIU Qiaoming1,2,*()   

  1. 1. School of Medicine and Health, Harbin Institute of Technology, Harbin 150001, China
    2. Zhengzhou Research Institute, Harbin Institute of Technology, Zhengzhou 450000, China
    3. The Shennong Laboratory, Zhengzhou 450002, China
    4. College of Animal Science and Technology, Henan Agricultural University, Zhengzhou 450046, China
    5. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2024-10-03 Online:2025-03-23 Published:2025-04-02
  • Contact: LI Guoxi, WANG Dandan, LIU Qiaoming E-mail:yuanfangli1991@163.com;liguoxi0914@126.com;wangdd1993@126.com;cslqm@hit.edu.cn

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Abstract:

This study aimed to investigate the effect of riboflavin (also known as vitamin B2, VB2) on the development of skeletal muscle in chicken embryos to promote its application in embryo feeding. The CCK-8, EdU, and immunofluorescence detection methods were used to identify the effects of adding different concentrations of VB2 (1.562 5, 3.125, 6.25, 12.5 and 25 μmol ·L-1) on the proliferation and differentiation of chicken primary myoblasts. Different concentrations of VB2 solution (50, 100 and 200 μg) were injected into the yolk sac of 7 embryonic age chicken embryos, and a 0.9% saline control group was set up to detect the effect of VB2 on the development of skeletal muscle in chicken embryos. The experiment was divided into 4 groups, with 25 embryo eggs in each group. The results showed that adding different concentrations of VB2 at the cellular level promoted the proliferation activity of chicken primary myoblasts to varying degrees, but 6.25 μmol ·L-1 VB2 was the optimal addition concentration. In addition, adding 6.25 μmol ·L-1 VB2 significantly promoted the expression of proliferation marker genes CCND1 (P < 0.01), PCNA (P < 0.05), and CDK1 (P < 0.01), but had no significant effect on the expression of differentiation marker genes MYOD, MYOG, and MYHC (P>0.05); at the same time, EdU experiments also confirmed that adding 6.25 μmol ·L-1 VB2 at the cellular level could significantly promote the proliferation of myoblasts (P < 0.05). In addition, the experiment of injecting VB2 solution into the yolk sac of chicken embryos showed that the injection of 50 μg VB2 significantly increased the expression levels of proliferation marker genes CDK1 (P < 0.01), PCNA (P < 0.001) and differentiation marker genes MYOD (P < 0.05), MYOG (P < 0.05) in the breast muscle tissue of 18 embryonic age chicken embryos; at the same time, the addition of 50 μg VB2 also significantly increased the fiber diameter (P < 0.05) and breast muscle weight (P < 0.05) of the breast muscle of 18 embryonic age chicken embryos. The above results show that VB2 can promote the proliferation of chicken primary myoblasts and the development of the breast muscle of chicken embryos. Therefore, the VB2 is an important nutrient that affects the development of the breast muscle of chicken embryos. This study provides an important theoretical basis and application value for the early embryonic nutritional regulation of high-quality broilers in China.

Key words: riboflavin, skeletal muscle development, chicken, embryonic nutrition

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