2013—2018年广西地区猪伪狂犬病病毒gB、gE与TK基因遗传变异分析

doi:10.11843/j.issn.0366-6964.2020.04.017

Abstract

Abstract: To study the epidemic situation of pseudorabies (PR) and genetic variation of epidemic strains of pseudorabies virus(PRV)in Guangxi from 2013 to 2018, 714 target tissues of suspected PR-infected pigs in various regions of Guangxi were collected and tested by PCR and virus isolation. gB, gE and TK genes of isolated strains were amplified, cloned, sequenced and analyzed by DNAStar and MEGA 6.0. The results indicated that the positive rate of suspected tissues was 24.5% (175/714), and 38 PRV strains were isolated. PRV infection of Pig is common in the tested herds. There co-existed classical strains and the variant strains, and the mutant strains are dominant. gB, gE and TK genes of different subtypes of isolated strains had the same amino acid variation characteristics and had closer relationship with domestic mutant strains. It is speculated that there were two different recombinant forms of PRV strains in Guangxi. The first was recombinant by vaccine strains (gB gene) and mutant strains (gE gene). The other one, classical strain (TK gene) was replaced by vaccine strains (TK gene). In recent years, PRV infection was common in the pig farms in Guangxi, and the epidemic strains were mainly mutant strains. And there may be two PRV recombinant forms of recombinant variant strains. Pig farms should pay more attention to practice PR targeted immunity plan.

Key words: porcine pseudorabies virus (PRV), gB gene, gE gene, TK gene, sequence analysis, genetic evolution analysis

CLC Number:

S852.659.1

ZHAO Shuo, WANG Ruomu, DANG Jiajia, XU Lishi, QIN Shuying, XUE Hui, WEI Zuzhang, CHEN Ying, OUYANG Kang, HUANG Weijian. Genetic Variation Analysis of gB, gE and TK Genes of Pseudorabies Virus in Guangxi from 2013 to 2018[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 810-819.

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Genetic Variation Analysis of gB, gE and TK Genes of Pseudorabies Virus in Guangxi from 2013 to 2018

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