SOCS2对山羊鼻甲骨细胞增殖、周期及凋亡的影响

doi:10.11843/j.issn.0366-6964.2024.05.040

Abstract

Abstract: Suppressor of cytokine signaling 2 (SOCS2) is involved in regulating various physiological and pathological processes in animals, such as cell apoptosis, proliferation, cell cycle, tumor, and inflammatory response. In this study, based on the first cloned SOCS2 gene from goats, the effects of overexpression and interference SOCS2 expression on proliferation, cycle and apoptosis of goat turbinate bone cells were studied. A total of 9 samples of heart, liver, spleen, lung, kidney, rumen, large intestine, small intestine and goat turbinate bone cells of healthy Jianzhou goats (6 animals) were used as samples, and the sequence of the CDS region of the goat SOCS2 gene was cloned by reverse transcription PCR and analyzed the sequence. RT-qPCR was used to detect the expression of SOCS2 gene in different tissues. The cloned SOCS2 gene CDS region ligation vector was constructed as a eukaryotic expression vector pcDNA3.1-SOCS2. Design, synthesis and screen effective siRNAs based on goat SOCS2 gene sequences. After transfecting pcDNA3.1-SOCS2 and siRNAs to turbinate osteocytes with Lipofectamine^TM 3000 reagent, SOCS2 protein expression was detected with Western blot mothed, the MTT method detects the effect of SOCS2 on cell proliferation, flow cytometry to detect the effect of SOCS2 on cell cycle and apoptosis and RT-qPCR was used to detect the transcription levels of cell cycle-related genes p21, CDK2 and apoptosis-related genes Caspase3, Caspase7, PARP1, p53, Bax, BCL2L11 and Bcl-2. The results showed that the total length of goat SOCS2 gene was 1 065 bp, and the CDS zone length was 597 bp, encoding 198 amino acid residues. SOCS2 gene is expressed at the highest levels in the liver tissue; SOCS2 can inhibit cell proliferation. The eukaryotic expression vector pcDNA3.1-SOCS2 was successfully constructed, and the gene was successfully expressed in turbinate bone cells. SOCS2 overexpression can lead to a significant increase in the number of cells in the G2/M+S phase and down-regulation of mRNA expression in CDK2 and p21 genes. It promoted apoptosis and upregulated the mRNA expression of Caspase3, Caspase7, Bax, p53, BCL2L11, and Bcl-2 genes, but there was no significant change in PARP1 expression level. However, after interference the expression of SOCS2, the number of cells in G0/G1 stage increased significantly, and the mRNA expression of CDK2 and p21 genes was upregulated. The apoptosis was inhibited and the mRNA expression of Caspase3, Bax, p53 and PARP1 genes was downregulated, while the expression levels of Caspase7, BCL2L11 and Bcl-2 did not change significantly. In summary, SOCS2 can block cells in the G2/M+S phase, promote apoptosis, and inhibit cell proliferation. The results of this study accumulate experimental data for a comprehensive reveal of the function of goat SOCS2.

Key words: goat, SOCS2, cell proliferation, cell cycle, apoptosis

CLC Number:

S852.21

LI Qiuyun, TIAN Xinyuan, LIAO Wensheng, ZHANG Huanrong, REN Yupeng, YANG Falong, ZHU Jiangjiang, XIANG Hua. Effects of SOCS2 on Proliferation, Cycle and Apoptosis of Turbinate Bone Cells in Goats[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2226-2240.

References

[1] 赵心愉, 刘娟, 邹曙明. 利用CRISPR/Cas9基因编辑技术敲除团头鲂SOCS1重复基因[J]. 水产学报, 2020, 44(12):1937-1947.
ZHAO X Y, LIU J, ZOU S M. Knock-out analysis of duplicated socs1 using CRISPR/Cas9 in Megalobrama amblycephala[J]. Journal of Fisheries of China, 2020, 44(12):1937-1947. (in Chinese)
[2] ZHANG B G, HU L, ZANG M D, et al. Helicobacter pylori CagA induces tumor suppressor gene hypermethylation by upregulating DNMT1 via AKT-NFκB pathway in gastric cancer development[J]. Oncotarget, 2016, 7(9):9788-9800.
[3] WAN J, CHE Y, KANG N N, et al. SOCS3 blocks HIF-1α expression to inhibit proliferation and angiogenesis of human small cell lung cancer by downregulating activation of Akt, but not STAT3[J]. Mol Med Rep, 2015, 12(1):83-92.
[4] QIN H W, HOLDBROOKS A T, LIU Y D, et al. SOCS3 deficiency promotes M1 macrophage polarization and inflammation[J]. J Immunol, 2012, 189(7):3439-3448.
[5] 周月宏, 田坚, 沈静雪, 等. SOCS2基因转染对人肾小球系膜细胞TGF-β、Ⅳ型胶原蛋白、纤维连接蛋白表达的影响[J]. 山东医药, 2016, 56(3):17-19.
ZHOU Y H, TIAN J, SHEN J X, et al. Effect of SOCS2 gene transfection on expression of TGF-β, collagen Ⅳ and FN in human glomerular mesangial cells[J]. Shandong Medical Journal, 2016, 56(3):17-19. (in Chinese)
[6] WANG S N, CAI Y L, BU R S, et al. PPARγ regulates macrophage polarization by inhibiting the JAK/STAT pathway and attenuates myocardial ischemia/reperfusion injury in vivo[J]. Cell Biochem Biophys, 2023, 81(2):349-358.
[7] 刘旭, 王海龙, 陈洪涛, 等. MiR-467b调控JAK/STAT信号通路在脂多糖诱导ATDC5细胞增殖及炎症反应中的作用[J]. 临床与病理杂志, 2021, 41(3):503-509.
LIU X, WANG H L, CHEN H T, et al. Role of miR-467b in regulating JAK/STAT signaling pathway in LPS induced proliferation and inflammatory response of ATDC5 cells[J]. Journal of Clinical and Pathological Research, 2021, 41(3):503-509. (in Chinese)
[8] 廉政君, 常炜. 汉黄芩素调节JAK/STAT信号通路并诱导肺癌A549细胞凋亡及周期阻滞的作用及机制研究[J]. 临床肺科杂志, 2021, 26(2):270-274, 279.
LIAN Z J, CHANG W. Effect and mechanism of wogonin on regulating JAK/STAT signal pathway and inducing apoptosis and cell cycle arrest in lung cancer A549 cells[J]. Journal of Clinical Pulmonary Medicine, 2021, 26(2):270-274, 279. (in Chinese)
[9] TANG Y J, WU W, CHEN Q Q, et al. miR-29b-3p suppresses the malignant biological behaviors of AML cells via inhibiting NF-κB and JAK/STAT signaling pathways by targeting HuR[J]. BMC Cancer, 2022, 22(1):909.
[10] 袁宇晴, 娄余, 龙丹, 等. 溃结宁膏调控microRNA-155介导JAK/STAT通路对HT-29细胞炎症模型的影响及作用机制研究[J]. 中国中西医结合消化杂志, 2023, 31(4):285-292.
YUAN Y Q, LOU Y, LONG D, et al. Mechanism of Kuijiening Plaster on inflammation model of HT-29 cells by regulating microRNA-155 mediated JAK/STAT pathway[J]. Chinese Journal of Integrated Traditional and Western Medicine on Digestion, 2023, 31(4):285-292. (in Chinese)
[11] ZADJALI F, SANTANA-FARRE R, VESTERLUND M, et al. SOCS2 deletion protects against hepatic steatosis but worsens insulin resistance in high-fat-diet-fed mice[J]. FASEB J, 2012, 26(8):3282-3291.
[12] YANG H L, YAN J, FENG M, et al. Construction of suppressor of cytokine signaling 2 (SOCS2) adenoviral overexpression vector and its impact on growth-hormone-induced lipolysis in swine primary adipocytes[J]. Genet Mol Res, GMR, 2013, 12(2):1283-1293.
[13] 成诚, 王佩雯, 杨益, 等. 肿瘤相关巨噬细胞在吸烟促进肺癌发展中作用及表观遗传机制[C]//中国毒理学会第十次全国毒理学大会论文集. 珠海:中国毒理学会, 2023:549-550.
CHENG C, WANG P W, YANG Y, et al. Role and epigenetic mechanism of tumor-associated macrophages in promoting the development of lung cancer through smoking[C]//Proceedings of the 10th National Toxicology Conference of the Chinese Society of Toxicology. Zhuhai:Chinese Society of Toxicology, 2023:549-550. (in Chinese)
[14] TU Y H, MEI F. miR-3648 promotes lung adenocarcinoma-genesis by inhibiting SOCS2 (suppressor of cytokine signaling 2)[J]. Bioengineered, 2022, 13(2):3044-3056.
[15] 戴奇山, 朱建国, 江福能, 等. SOCS2在良性前列腺增生症与前列腺癌中的表达及其意义[J]. 中国现代医学杂志, 2013, 23(34):11-15.
DAI Q S, ZHU J G, JIANG F N, et al. Expression and significances of SOCS2 in prostate cancer tissues and benign prostate hyperplasia tissues[J]. China Journal of Modern Medicine, 2013, 23(34):11-15. (in Chinese)
[16] ZHOU X H, XU H, XU C, et al. Hepatocellular carcinoma-derived exosomal miRNA-761 regulates the tumor microenvironment by targeting the SOCS2/JAK2/STAT3 pathway[J]. World J Emerg Med, 2022, 13(5):379-385.
[17] 李晓艳, 莫立稳, 程莹, 等. 白果内酯调控细胞因子信号转导抑制因子2抑制高糖诱导的肾小球系膜细胞中炎症与纤维化成分的表达[J]. 河北医学, 2022, 28(12):2007-2012.
LI X Y, MO L W, CHENG Y, et al. Bilobalide regulates SOCS2 to inhibit the expression of inflammatory fibrotic components in high glucose-induced glomerular mesangial cells[J]. Hebei Medicine, 2022, 28(12):2007-2012. (in Chinese)
[18] LINOSSI E M, CALLEJA D J, NICHOLSON S W. Understanding SOCS protein specificity[J]. Growth Factors, 2018, 36(3-4):104-117.
[19] DURHAM G A, WILLIAMS J J L, NASIM M T, et al. Targeting SOCS proteins to control JAK-STAT signalling in disease[J]. Trends Pharmacol Sci, 2019, 40(5):298-308.
[20] 解芸菲. siRNA干扰SOCS3表达对TNF-α诱导前体脂肪细胞凋亡的影响[D]. 杨凌:西北农林科技大学, 2011.
XIE Y F. Effects of Silencing SOCS3 expression by SIRNA on TNF-α induced preadipocytes apoptosis[D]. Yangling:Northwest A&F University, 2011. (in Chinese)
[21] DUAN Y C, CHEN L, SHAO J, et al. Lanatoside C inhibits human cervical cancer cell proliferation and induces cell apoptosis by a reduction of the JAK2/STAT6/SOCS2 signaling pathway[J]. Oncol Lett, 2021, 22(4):740.
[22] 李胜. STAT5对水牛乳腺上皮细胞增殖和乳蛋白基因表达的调控作用及乳腺组织的定量蛋白质组学研究[D]. 南宁:广西大学, 2019.
LI S. Studies related to actions of stat5 in proliferation of mammary epithielal cells and expression of genes related to milk proteins and quantitative proteomics of buffalo mammary gland[D]. Nanning:Guangxi University, 2019. (in Chinese)
[23] LI S, HAN S, JIN K P, et al. SOCS2 suppresses inflammation and apoptosis during NASH progression through limiting NF-κB activation in macrophages[J]. Int J Biol Sci, 2021, 17(15):4165-4175.
[24] 王宪军, 向华, 张焕容, 等. 山羊HABP4基因的克隆、序列分析及功能预测[J]. 畜牧兽医学报, 2021, 52(12):3426-3438.
WANG X J, XIANG H, ZHANG H R, et al. Cloning, sequence analysis and function prediction of HABP4 gene in goat[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12):3426-3438. (in Chinese)
[25] 徐颂为, 向华, 张焕容, 等. 干扰SERBP1表达对山羊鼻甲骨原代细胞增殖、周期及凋亡的作用[J]. 畜牧兽医学报, 2022, 53(8):2708-2720.
XU S W, XIANG H, ZHANG H R, et al. Effects of interfering SERBP1 on the cell proliferation, cell cycle and apoptosis in goat turbinate bone primary cells[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8):2708-2720. (in Chinese)
[26] 王江林, 王永, 池永东, 等. KLF11抑制山羊肌内前体脂肪细胞分化[J]. 畜牧兽医学报, 2020, 51(5):976-986.
WANG J L, WANG Y, CHI Y D, et al. KLF11 inhibits the differentiation of goat intramuscular preadipocyte[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(5):976-986. (in Chinese)
[27] 王宪军, 向华, 张旭梅, 等. 羊口疮病毒ORFV118蛋白对山羊睾丸支持细胞周期、凋亡及免疫细胞因子的影响[J]. 华北农学报, 2022, 37(1):222-231.
WANG X J, XIANG H, ZHANG X M, et al. Effects of ORFV118 on cell cycle, apoptosis and the immune-related cytokines in goat testicular sertoli cells[J]. Acta Agriculturae Boreali-Sinica, 2022, 37(1):222-231. (in Chinese)
[28] 但一昕, 杨璐, 向华, 等. BIRC5对山羊睾丸细胞周期、凋亡的影响[J]. 畜牧兽医学报, 2023, 54(4):1511-1524.
DAN Y X, YANG L, XIANG H, et al. Effects of BIRC5 on the cycle and apoptosis of goat testis cells[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4):1511-1524. (in Chinese)
[29] 刘海涛, 罗庆, 范立, 等. 细胞因子信号转导负调控蛋白2在变应性鼻炎中的表达及临床意义[J]. 中国医药导报, 2019, 16(33):19-22.
LIU H T, LUO Q, FAN L, et al. Expression and clinical significance of negative regulatory protein 2 of cytokine signal transduction in allergic rhinitis[J]. China Medical Herald, 2019, 16(33):19-22. (in Chinese)
[30] 董宝霞, 杨玉莹, 荆海霞, 等. 瘦素对牦牛MEC中SOCS2 mRNA及蛋白水平的表达影响[J]. 甘肃农业大学学报, 2022, 57(4):10-16, 26.
DONG B X, YANG Y Y, JING H X, et al. Effect of leptin on SOCS2 mRNA and protein expression in MEC of yak[J]. Journal of Gansu Agricultural University, 2022, 57(4):10-16, 26. (in Chinese)
[31] 贾超, 李树岗, 范秋玉, 等. LncRNA Gm15621通过调控miR-133a/SOCS2轴抑制类风湿关节炎成纤维样滑膜细胞炎症[J]. 免疫学杂志, 2021, 37(3):263-269.
JIA C, LI S G, FAN Q Y, et al. LncRNA Gm15621 inhibits fibroblast-like synoviocytes inflammation by regulating miR-133a/SOCS2 axis in rheumatoid arthritis[J]. Immunological Journal, 2021, 37(3):263-269. (in Chinese)
[32] DIEL D G, LUO S, DELHON G, et al. Orf virus ORFV121 encodes a novel inhibitor of NF-κB that contributes to virus virulence[J]. J Virol, 2011, 85(5):2037-2049.
[33] WANG X J, XIANG H, ZHANG H R, et al. HABP4 overexpression promotes apoptosis in goat turbinate bone cells[J]. Anim Biotechnol, 2022, doi:10.1080/10495398.2022.2062601.
[34] 马涛, 王红梅, 赵婷, 等. 阿曼托双黄酮通过JAK2-STAT3通路影响甲状腺癌SW579细胞的增殖和凋亡[J]. 中国肿瘤生物治疗杂志, 2023, 30(3):211-216.
MA T, WANG H M, ZHAO T, et al. Amentoflavone induces apoptosis and suppresses proliferation of thyroid carcinoma SW579 cells by inhibiting the activation of JAK2-STAT3 pathway[J]. Chinese Journal of Cancer Biotherapy, 2023, 30(3):211-216. (in Chinese)
[35] 施姮, 舒芳芳. 连翘苷调控细胞因子信号转导抑制因子1对肺炎链球菌诱导的肺泡上皮细胞凋亡和氧化应激的影响[J]. 中国临床药理学杂志, 2023, 39(8):1142-1146.
SHI H, SHU F F. Effects of phillyrin on alveolar epithelial cell apoptosis and oxidative stress induced by Streptococcus pneumoniae by regulating suppressor of cytokine signaling 1[J]. The Chinese Journal of Clinical Pharmacology, 2023, 39(8):1142-1146. (in Chinese)
[36] XIAO C W, ZHANG W, HUA M M, et al. RNF7 inhibits apoptosis and sunitinib sensitivity and promotes glycolysis in renal cell carcinoma via the SOCS1/JAK/STAT3 feedback loop[J]. Cell Mol Biol Lett, 2022, 27(1):36.
[37] 谭慧心, 董彦宏, 关文辉, 等. 细胞周期调控与肿瘤发生和治疗的关系研究[J]. 哈尔滨商业大学学报(自然科学版), 2015, 31(6):667-670.
TAN H X, DONG Y H, GUAN W H, et al. Relation of cell cycle regulation with occurrence and treatment of tumor[J]. Journal of Harbin University of Commerce (Natural Sciences Edition), 2015, 31(6):667-670. (in Chinese)
[38] KIM M Y, PARK S J, SHIM J W, et al. Naphthazarin enhances ionizing radiation-induced cell cycle arrest and apoptosis in human breast cancer cells[J]. Int J Oncol, 2015, 46(4):1659-1666.
[39] CHEN S H, LUO T W, YU Q, et al. Isoorientin plays an important role in alleviating cadmium-induced DNA damage and G0/G1 cell cycle arrest[J]. Ecotoxicol Environ Saf, 2020, 187:109851.
[40] 彭胜建, 丁美胜, 赵世明, 等. 过表达细胞因子信号转导抑制因子2调控JAK2/STAT3通路抑制直肠癌细胞活性和移植瘤生长[J]. 重庆医科大学学报, 2023, 48(1):37-42.
PENG S J, DING M S, ZHAO S M, et al. Overexpression of SOCS2 on inhibition of rectal cancer cell growth activity and xenograft tumor growth by JAK2/STAT3 pathway[J]. Journal of Chongqing Medical University, 2023, 48(1):37-42. (in Chinese)
[41] 谢娜娜, 闫书平, 张崇昊, 等. 植物雌激素大豆黄酮对牛乳腺上皮细胞增殖及细胞周期的影响[J]. 动物营养学报, 2022, 34(4):2645-2653.
XIE N N, YAN S P, ZHANG C H, et al. Effects of phytoestrogen daidzein on proliferation and cell cycle of bovine mammary epithelial cells[J]. Chinese Journal of Animal Nutrition, 2022, 34(4):2645-2653. (in Chinese)
[42] 潘剑锋, 尚方正, 马荣, 等. 周期蛋白和周期蛋白依赖性激酶及相关激酶抑制剂在细胞周期进程中的调控机制研究进展[J]. 生物工程学报, 2023, 39(4):1525-1547.
PAN J F, SHANG F Z, MA R, et al. Advances of the regulatory mechanism of cyclin, cyclin- dependent kinases and related kinase inhibitors in cell cycle progression[J]. Chinese Journal of Biotechnology, 2023, 39(4):1525-1547. (in Chinese)
[43] 范金柱, 郭云山, 任小宇, 等. AUF1负向调控p21对骨肉瘤细胞增殖和凋亡的影响[J]. 西部医学, 2023, 35(4):480-484, 489.
FAN J Z, GUO Y S, REN X Y, et al. The effect of AUF1 negatively regulating p21 on the proliferation and apoptosis of osteosarcoma cells[J]. Medical Journal of West China, 2023, 35(4):480-484, 489. (in Chinese)
[44] LEI T J, ZHANG P X, ZHANG X D, et al. Cyclin K regulates prereplicative complex assembly to promote mammalian cell proliferation[J]. Nat Commun, 2018, 9(1):1876.
[45] LI M, GAO P, ZHANG J P. Crosstalk between autophagy and apoptosis: potential and emerging therapeutic targets for cardiac diseases[J]. Int J Mol Sci, 2016, 17(3):332.
[46] OUYANG C H, YOU J Y, XIE Z L. The interplay between autophagy and apoptosis in the diabetic heart[J]. J Mol Cell Cardiol, 2014, 71:71-80.
[47] MARQUEZ R T, XU L. Bcl-2: Beclin 1 complex: multiple, mechanisms regulating autophagy/apoptosis toggle switch[J]. Am J Cancer Res, 2012, 2(2):214-221.
[48] HIRUNAGI T, SAHASHI K, TACHIKAWA K, et al. Selective suppression of polyglutamine-expanded protein by lipid nanoparticle-delivered siRNA targeting CAG expansions in the mouse CNS[J]. Mol Ther-Nucl Acids, 2021, 24:1-10.
[49] SHI L, HU H B, SUN P X, et al. RPL38 knockdown inhibits the inflammation and apoptosis in chondrocytes through regulating METTL3-mediated SOCS2 m6A modification in osteoarthritis[J]. Inflamm Res, 2022, 71(7-8):977-989.
[50] LALIER L, CARTRON P F, JUIN P, et al. Bax activation and mitochondrial insertion during apoptosis[J]. Apoptosis, 2007, 12(5):887-896.

Effects of SOCS2 on Proliferation, Cycle and Apoptosis of Turbinate Bone Cells in Goats

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