甘草多糖对LPS诱导小鼠睾丸炎的调节作用

doi:10.11843/j.issn.0366-6964.2024.06.044

Abstract

Abstract:

The present study aimed to investigate the regulation and its possible mechanism of glycyrrhiza polysaccharide (GPS) on LPS-induced orchitis in mice. Sixty 8-week-old male C57BL/6J mice were randomly divided into 6 groups (10 mice per group): Blank control group, model group (LPS group), positive control group (dexamethasone group) and GPS low, medium, high dose (100, 200, 400 mg·kg^-1) group. On the first 20 days, GPS low, medium, high dose group was given the corresponding dose of GPS by intragastric, and other groups were given the same dose of physiological saline by intragastric. On the 21st day, model group was intraperitoneally injected with 5 mg·kg^-1 LPS, and other groups were intraperitoneally injected with the same amount of physiological saline, 2 h later, the positive control group was given 5 mg·kg^-1 dexamethasone by intragastric, 12 h later, the mice were sacrificed according to animal ethical standards, blood and testicular tissues were collected. The morphological changes of testicular tissue were observed by HE staining, the content of testosterone (T) and the level of lactate dehydrogenase (LDH) in serum were detected by enzyme-linked immunosorbent assay (ELISA), and the changes of oxidative damage indexes (T-SOD, CAT, GSH) were detected by Nanjing Jiancheng Kit. Real-time fluorescence quantitative PCR was used to detect mRNA expression changes of tight junction proteins (Occludin, ZO-1), inflammatory factors (IL-18, IL-1β, IL-6, TNF-α) and key cytokines of pyroptosis (caspase-1, NLRP3, GSDMD) in testis, western blot was used to detect the protein expression of IL-18, IL-1β, NLRP3, GSDMD. The results showed that dexamethasone group and GPS medium, high dose groups could significantly relieve the LPS-induced testicular damage, increase the serum testosterone content, reduce oxidative damage, decrease the mRNA expressions of pro-inflammatory cytokines, and inhibit the occurrence of pyroptosis. In summary, GPS could effectively alleviate LPS-induced orchitis injury, which may related to reducing oxidative damage, inhibiting pyroptosis and preventing inflammatory response.

Key words: glycyrrhizol polysaccharide, LPS, orchitis, pyroptosis

CLC Number:

S853.74

Meiyan LI, Xiaoying XING, Na LI, Yeli ZHANG, Hongping QIAO, Xiaoying WU. Regulation of Glycyrrhizol Polysaccharide Alleviate LPS-induced Orchitis in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(6): 2741-2750.

Figures/Tables 7

Table 1

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References 31

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引物名称 Name	引物序列(5′→3′) Sequence	引物大小/bp Product size
β-actin	F：TACCCAGGCATTGCTGACAG R：ACTCCTGCTTGCTGATCCAC	169
IL-1β	F：TCAGCACCTCACAAGCAGAG R：TTCTTGTGACCCTGAGCGAC	230
IL-18	F：ATGCTTTCTGGACTCCTGCC R：AGTCTTCTGACATGGCAGCC	149
IL-6	F：TTGGGACTGATGCTGGTGAC R：AGACAGGTCTGTTGGGAGTG	94
TNF-α	F：TGTCCCTTTCACTCACTGGC R：TCTTCTGCCAGTTCCACGTC	98
ZO-1	F：AAACAGCCCTACCAACCTCG R：TTCGAGGCAGCTGCTCATAG	163
Occludin	F：TTGACTGGGCTGAACACTCC R：ACATCACAGCTCACACCAGG	122
Caspase-1	F：AGTTGCTGCTGGAGGATCTG R：AGAAGTCTTGTGCTCTGGGC	113
NLRP3	F：TCTGCACCCGGACTGTAAAC R：CATTGTTGCCCAGGTTCAGC	131
GSDMD	F：TGCGTGTGACTCAGAAGACC R：AAACAGGTCATCCCCACGAC	107

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Regulation of Glycyrrhizol Polysaccharide Alleviate LPS-induced Orchitis in Mice

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