BCG感染巨噬细胞后内质网应激对细胞焦亡的调控作用

doi:10.11843/j.issn.0366-6964.2021.010.015

Abstract

Abstract: The purpose of this research is to provide the regulation and mechanism of endoplasmic reticulum stress(ERS) on pyroptosis of THP-1 cells infected by BCG. THP-1 cells were infected by BCG with or without TUDCA treatment. Then, the mRNA expression of GRP78, GSDMD, Caspase1, NLRP3, IL-1β, IL-18, the protein expression of GRP78, Caspase12, GSDMD, Caspase1, NLRP3, the release of IL-1β, IL-18 and the cell survival rate were detected by qRT-PCR, Western blot, ELISA and CCK-8 respectively. The results showed that both the mRNA and the protein expressions of GRP78 were gradually increased with time of BCG infection in THP-1 cells (P<0.01). The protein expression of GSDMD reached the highest level at 24 h post infection. The concentration of IL-1β and IL-18 was increased in a time-dependent manner (P<0.01). Compared with the uninfected group, the mRNA expression of GRP78, GSDMD, Caspase1, NLRP3, IL-1β and IL-18 (P<0.05), and the protein expression of GRP78, Caspase12, GSDMD, Caspase1 and NLRP3 (P<0.001) were both up-regulated, and the concentration of IL-1β and IL-18 was increased (P<0.01), and cells survival rate was reduced at 24 h post infection with BCG (P<0.001). However, with the co-treatment of TUDCA and BCG, the expression of the above molecules decreased and the cell survival rate increased compared with BCG infected group (P<0.01). Our results demonstrate that ERS in THP-1 cells induced by BCG infection regulated pyroptosis.

Key words: BCG, endoplasmic reticulum stress, pyroptosis, THP-1 cells

CLC Number:

NIE Xueyi, LIU Lei, ZHENG Xuedi, YANG Yi, XU Jinrui, WANG Yujiong. Role of Endoplasmic Reticulum Stress on Pyroptosis of BCG Infected Macrophages[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(10): 2842-2851.

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Role of Endoplasmic Reticulum Stress on Pyroptosis of BCG Infected Macrophages

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