脂肪间充质干细胞对小型猪肝缺血再灌注合并肝切除组织细胞焦亡的影响

doi:10.11843/j.issn.0366-6964.2024.01.033

Abstract

Abstract: The purpose of this study was to investigate the effect of adipose derived mesenchymal stem cells (ADSCs) on pyroptosis of miniature pigs with liver Ischemia-reperfusion injury (IRI) combined with hepatectomy. Eighteen Bama mini pigs were randomly divided into three groups, 6 in each group, which were Sham surgery group (sham group), model group (IRI group) and ADSCs intervention group (ADSCs group, dose is 1×10⁶ cells·kg^-1). The model of liver IRI combined with hepatectomy injury was established by laparoscopic minimally invasive technique. The IRI group and ADSCs group mini pigs were injected with normal saline and ADSCs into the liver parenchyma immediately after operation. Blood and liver tissue samples were collected 1, 3, and 7 d after surgery. Application of ELISA method to detect pro-inflammatory factors IL-18 and IL-1β in serum. The content of pyroptosis related genes and proteins were detected by RT-qPCR and Western blot. The results showed that on the 1 and 3 d, compared to the sham group, the serum pro-inflammatory factors IL-18 and IL-1β in the IRI group were significantly higher than those in the sham group (P<0.01). The expression levels of IL-18 and IL-1β genes and proteins in liver tissue were also significantly increased (P<0.01); The levels of the pyroptosis related factors NOD-like receptor protein 3 (NLRP3), apoptosis-associated speck-like protein containing a CARD (ASC), Caspase-1, GSDMD and nuclear factor kappa-Bp65 (NF-κBp65) genes and proteins in liver tissue were significantly elevated (P<0.05). After intervention with ADSCs, the expression levels of IL-18 and IL-1β in serum and liver tissue were significantly reduced (P<0.05). The gene and protein expression of pyroptosis related factors NLRP3, ASC, Caspase-1, GSDMD and NF-κBp65 in liver tissue was significantly reduced (P<0.05). Therefore, this study proves that laparoscopic liver IRI combined with hepatectomy injury can induce pyroptosis in miniature pigs, and ADSCs can reduce and improve the pyroptosis injury caused by this liver injury.

Key words: adipose derived mesenchymal stem cells, liver ischemia-reperfusion injury, pyroptosis, laparoscope, miniature pig

CLC Number:

S854

MA Yajun, JIAO Zhihui, LIU Xiaoning, LU Xiangyu, LIU Tao, WANG Yue, PIAO Chenxi, WANG Hongbin. Effects of Adipose-derived Mesenchymal Stem Cells on Pyroptosis of Miniature Pigs with Hepatic Ischemia-Reperfusion Combined with Hepatectomy[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 355-364.

References 13

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Effects of Adipose-derived Mesenchymal Stem Cells on Pyroptosis of Miniature Pigs with Hepatic Ischemia-Reperfusion Combined with Hepatectomy

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