蒲公英提取物对LPS诱导小鼠乳腺炎的减轻效应及其机制分析

doi:10.11843/j.issn.0366-6964.2022.08.034

Abstract

Abstract: The purpose of this study was to study the alleviating effect of dandelion extract on lipopolysaccharide (LPS) induced mastitis in mice and its mechanism. The mice were divided into blank group, model group, positive group (DEX) and dandelion extract high, medium and low dose groups. Dandelion extract was administered at high, medium and low doses according to 10.0, 5.0, 2.5 g·kg^-1 gastric lavage, continuous gastric lavage for 6 days, 2 times per day, and the blank group was gastrically filled with equal volume of normal saline. One hour after the last administration, 50 μL of 0.2 mg·mL^-1 LPS was perfused in the basal part of the breast of mice to establish LPS-induced model of mastitis in mice, and the positive group was injected 5 mg·kg^-1 of dexamethasone intraperitoneally 6 and 12 h after modeling. Twenty-four hours later, blood was collected and serum was separated, and breast tissue was stripped. The contents of serum tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and interleukin-6 (IL-6) were determined by ELISA, the content of myeloperoxidase (MPO) was measured by MPO kit, the pathological change was observed by HE staining, the expressions of TLR4 protein and related proteins of NF-κB and MAPKs signaling pathways in mouse mammary glands were measured by Western blot. The results showed that dandelion extract at high and medium dose groups significantly inhibited the secretion of serum TNF-α, IL-1β and IL-6 (P<0.01), reduced MPO content in LPS-induced mastitis mice (P<0.01), and dandelion at high and medium dose groups extract improved pathological changes of mastitis induced by LPS, significantly down-regulated the expression of TLR4, p-IκB, p-p65, p-p38, p-JNK, p-ERK proteins in LPS-induced mastitis (P<0.01). The results showed that dandelion extract can significantly alleviate LPS induced mastitis by regulating NF-κB and MAPKs signaling pathways in mice, which lays a foundation for the clinical development and application of dandelion.

Key words: Dandelion extract, LPS, mastitis, NF-κB, MAPKs

CLC Number:

S853.74

ZHAO Xin, WANG Ying, LI Chunting, WANG Wei, SANG Rui, LI Haitao, ZHANG Xuemei. Alleviating Effect and Mechanism of Dandelion Extract on LPS-Induced Mastitis in Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2773-2781.

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Alleviating Effect and Mechanism of Dandelion Extract on LPS-Induced Mastitis in Mice

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