白头翁汤通过抑制TLR4-ERK1/2信号通路缓解LPS诱导的微血管内皮细胞炎性反应

doi:10.11843/j.issn.0366-6964.2021.08.025

Abstract

Abstract: In recent years, the researches on the anti-inflammatory mechanisms of Traditional Chinese Medicine formula have been in-depth. Pulsatilla decoction served as a classic Traditional Chinese Medicine prescription for clearing away heat and toxic material, is usually employed to prevent and treat bacterial diarrhea. However, its anti-inflammatory mechanisms and target cells are still unclear. In this study, rat intestinal mucosa microvascular endothelial cells (RIMVECs) were used as the model cells, aiming to illustrate the regulation effects of Pulsatilla decoction on LPS-induced inflammation of RIMVECs. The levels of mRNA and protein expressions of key proteins TLR4, TRAF6 and ERK in the inflammatory signaling pathway of RIMVECs after LPS stimulation were detected by RT-PCR and Western blot. The release levels of IL-6, IL-8, IL-1β, and TNF-alpha were determined by ELISA. The results showed that Pulsatilla decoction could significantly reduce the mRNA levels and protein expressions of TLR4, TRAF6 and ERK induced by LPS, and also suppress the secretions of the downstream inflammatory factors induced by LPS. Pulsatilla decoction alleviates the inflammatory responses of LPS-induced RIMVECs by inhibiting TLR4-ERK1/2 signaling pathway and plays an anti-inflammatory role.

Key words: microvascular endothelial cells, LPS, Pulsatilla decoction, ERK1/2 signaling pathway, PD98059

CLC Number:

S853.91

HE Shangwen, WANG Yueming, ZHANG Hui, HOU Silu, LIU Xiaoye, DONG Hong. Pulsatilla Decoction Alleviates LPS-induced Inflammation of Microvascular Endothelial Cells by Inhibiting the TLR4-ERK1/2 Signaling Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2317-2325.

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Pulsatilla Decoction Alleviates LPS-induced Inflammation of Microvascular Endothelial Cells by Inhibiting the TLR4-ERK1/2 Signaling Pathway

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