艾纳香油对NF-κB及Nrf2/HO-1信号通路的作用研究

doi:10.11843/j.issn.0366-6964.2021.04.013

Abstract

Abstract: The purpose of this study was to reveal the mechanism of anti-inflammatory effect of BBO by studying NF-κB and Nrf2/HO-1 pathway. RAW264.7 macrophage inflammation model induced by LPS was used. The control group, LPS model group, BBO group (low, middle and high dose) and BBO negative control group were set up in this experiment, with 3 repeats in each group. The effect of BBO on apoptosis was detected by Hoechst 33342 and PI double staining. The contents of IL-1β, TNF-α, PGE₂, LTB₄, NO and the activity of iNOS in LPS-induced macro-phage were detected by ELISA and spectrophotometry. The effect of BBO on the mRNA expression of TNF-α and IL-1β was detected by RT-PCR. The effect of BBO on the key proteins expression in NF-κB and Nrf2/HO-1 signal pathway was detected by Western blotting. The results showed that BBO could reverse the morphological changes and apoptosis of macrophages induced by LPS within the dose range of 80 μg·mL^-1. Compared with LPS model group, BBO of 60-80 μg·mL^-1 could extremely significantly inhibit the secretion of LPS-induced cellular inflammatory factors and inflammatory mediators IL-1β, TNF-α, PGE₂, LTB₄, NO and iNOS activity(P<0.01); BBO could also extremely significantly inhibit the expression of IL-1β and TNF-α mRNA(P<0.01) induced by LPS. At the same time, BBO of 60-80 μg·mL^-1 could extremely significantly down-regulate the protein expression of COX-2, 5-LOX, p-IKKα, p-P65, p-IκB-α and cytoplasmic Nrf2 induced by LPS(P<0.01), and extremely significantly promote the protein expression of HO-1 and nuclear Nrf2 in a dose-dependent manner(P<0.01). The results suggest that BBO has good effects on anti-inflammatory and anti-apoptotic. It may play an anti-inflammatory role by inhibiting the phosphorylation of key proteins and the production of inflammatory factors in NF-κB pathway and promote the expression of major anti-inflammatory genes in Nrf2/HO-1 pathway.

Key words: Blumea balsamifera(L.) DC Oil, LPS, RAW264.7 cell, NF-κB, Nrf2/HO-1 pathway

CLC Number:

S852.2

WANG Wanlin, GAO Yue, LIAO Jiamei, PENG Junchao, CAI Yaling, YI Qiong, WANG Lu. Effect of Blumea balsamifera (L.) DC Oil on NF-κB and Nrf2/HO-1 Signal Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 976-986.

References 38

[1]	张建,徐芬,郝华.脂加氧酶表达与肿瘤及炎症性疾病关系的研究进展[J].临床与实验病理学杂志,2017,33(6):666-668.ZHANG J,XU F,HAO H.Research progress on the relationship between lipoxygenase expression and tumor and inflammatory diseases[J].Chinese Journal of Clinical and Experimental Pathology,2017,33(6):666-668.(in Chinese)
[2]	WU Y B,KANG J J,ZHANG L,et al.Ubiquitination regulation of inflammatory responses through NF-κB pathway[J].Am J Transl Res,2018,10(3):881-891.
[3]	宋一萌,李明真,马潞林.花生四烯酸代谢产物与肿瘤发生和发展的研究进展[J].临床泌尿外科杂志.2017,32(3):236-240.SONG Y M,LI M Z,MA L L.Research advances in the association between arachidonic acid metabolites and tumorigenesis[J].Journal of Clinical Urology,2017,32(3):236-240.(in Chinese)
[4]	陶磊,傅淑霞.花生四烯酸与氧化应激的研究进展[J].中国病理生理杂志,2011,27(11):2233-2236.TAO L,FU S X.Progress on arachidonic acid and oxidative stress[J].Chinese Journal of Pathophy-siology, 2011,27(11):2233-2236.(in Chinese)
[5]	LOBODA A,DAMULEWICZ M,PYZA E,et al.Role of Nrf2/HO-1 system in development,oxidative stress response and diseases:an evolutionarily conserved mechanism[J].Cell Mol Life Sci,2016,73(17):3221-3247.
[6]	袁媛,庞玉新,王文全,等.中国艾纳香属植物资源与民族药学研究[J].热带农业科学,2011,31(4):22-27.YUAN Y,PANG Y X,WANG W Q,et al.Medical ethnobotany of the genus of Blumea. in China[J].Chinese Journal of Tropical Agriculture,2011,31(4):22-27.(in Chinese)
[7]	ZHANG B,TANG M H,ZHANG W H,et al.Chemical composition of Blumea balsamifera and Magnolia sieboldii essential oils and prevention of UV-B radiation-induced skin photoaging[J/OL].Natural Product Research,2020, doi:10.1080/14786419. 2020.1809401.
[8]	SAKEE U,MANEERAT S,CUSHNIE T P T,et al.Antimicrobial activity of Blumea balsamifera (Lin.) DC. extracts and essential oil[J].Nat Prod Res,2011,25(19):1849-1856.
[9]	HE C L,YANG P Y,WANG L,et al.Antibacterial effect of Blumea balsamifera DC. essential oil against Haemophilus parasuis[J].Arch Microbiol,2020,202(9):2499-2508.
[10]	谢雪艳,李天珍,王万林,等.不同产源艾纳香油化学成分及其抗炎活性研究[J].中药新药与临床药理,2019,30(9):1069-1076.XIE X Y,LI T Z,WANG W L,et al.Analysis of chemical constituents and anti-inflammatory activity of Blumea balsamifera oil from different sources[J].Traditional Chinese Drug Research and Clinical Pharmacology,2019,30(9):1069-1076.(in Chinese)
[11]	HE C L,ZHAO Y,JIANG X L,et al.Protective effect of Ketone musk on LPS/ATP-induced pyroptosis in J774A.1 cells through suppressing NLRP3/GSDMD pathway[J].Int Immunopharmacol,2019,71:328-335.
[12]	LAMKANFI M,DIXIT V M.Manipulation of host cell death pathways during microbial infections[J].Cell Host Microbe, 2010,8(1):44-54.
[13]	SALEH D,DEGTEREV A.Emerging roles for RIPK1 and RIPK3 in pathogen-induced cell death and host immunity[J].Curr Top Microbiol Immunol,2017,403:37-75.
[14]	POECKEL D,BERRY K A Z,MURPHY R C,et al.Dual 12/15- and 5-lipoxygenase deficiency in macro-phages alters arachidonic acid metabolism and attenuates peritonitis and atherosclerosis in ApoE knock-out mice[J].J Biol Chem,2009, 284(31):21077-21089.
[15]	JOSHI V,VENKATESHA S H,RAMAKRISHNAN C,et al.Celastrol modulates inflammation through inhibition of the catalytic activity of mediators of arachidonic acid pathway:Secretory phospholipase A2 group IIA,5-lipoxygenase and cyclooxygenase-2[J]. Pharmacol Res,2016,113:265-275.
[16]	PURATCHIKODY A,UMAMAHESWARI A,IRFAN N,et al.A novel class of tyrosine derivatives as dual 5-LOX and COX-2/mPGES1 inhibitors with PGE2 mediated anticancer properties[J].New J Chem,2019,43(2):834-846.
[17]	BRUNO F,SPAZIANO G,LIPARULO A,et al.Recent advances in the search for novel 5-lipo-xygenase inhibitors for the treatment of asthma[J].Eur J Med Chem,2018,153:65-72.
[18]	WU B L,BAI C Y,DU Z P,et al.The arachidonic acid metabolism protein-protein interaction network and its expression pattern in esophageal diseases[J].Am J Transl Res,2018,10(3):907-924.
[19]	KWON D H,CHA H J,CHOI E O,et al.Schisandrin A suppresses lipopolysaccharide-induced inflammation and oxidative stress in RAW 264.7 macrophages by suppressing the NF-κB,MAPKs and PI3K/Akt pathways and activating Nrf2/HO-1 signaling[J].Int J Mol Med,2018,41(1):264-274.
[20]	STOHL W,JACOB N,GUO S H,et al.Constitutive overexpression of BAFF in autoimmune-resistant mice drives only some aspects of systemic lupus erythe-matosus-like autoimmunity[J].Arthritis Rheum,2010,62(8):2432-2442.
[21]	JOSHI M,REDDY N D,KUMAR N,et al.Cinnamyl sulfonamide hydroxamate derivatives inhibited LPS-stimulated NF-kB expression in RAW 264.7 cells in vitro and mitigated experimental colitis in wistar rats in vivo[J].Curr Pharm Des,2020, 26(38):4934-4943.
[22]	CILDIR G,LOW K C,TERGAONKAR V.Non-canonical NF-κB signaling in health and disease[J].Trends Mol Med,2016, 22(5):414-429.
[23]	任改艳,张步有,黄剑林.槲皮素对LPS诱导小鼠RAW264.7细胞炎症的保护作用[J].中成药,2019,41(8):1795-1799.REN G Y,ZHANG B Y,HUANG J L.Protective effects of quercetin on the inflammation of mice RAW264.7 cells induced by LPS[J].Chinese Traditional Patent Medicine,2019,41(8):1795-1799.(in Chinese)
[24]	LIU F,XIA Y F,PARKER A S,et al.IKK biology[J].Immunol Rev,2012,246(1):239-253.
[25]	LIU B G,XIA X J,ZHU F,et al.IKKα is required to maintain skin homeostasis and prevent skin cancer[J].Cancer Cell, 2008,14(3):212-225.
[26]	王甜甜,陈淳媛,杨雷,等.Nrf2/HO-1信号轴在氧化应激性疾病中的机制[J].中南大学学报:医学版,2019,44(1):74-80.WANG T T,CHEN C Y,YANG L,et al.Role of Nrf2/HO-1 signal axis in the mechanisms for oxidative stress-relevant diseases[J]. Journal of Central South University:Medical Science,2019,44(1):74-80.(in Chinese)
[27]	KANG K A,HYUN J W.Oxidative stress,Nrf2,and epigenetic modification contribute to anticancer drug resistance[J].Toxicol Res,2017,33(1):1-5.
[28]	LOBODA A,DAMULEWICZ M,PYZA E,et al.Role of Nrf2/HO-1 system in development,oxidative stress response and diseases:an evolutionarily conserved mechanism[J].Cell Mol Life Sci,2016,73(17):3221-3247.
[29]	KLOSKA D,KOPACZ A,PIECHOTA-POLANCZYK A,et al.Nrf2 in aging-Focus on the cardiovascular system[J].Vascul Pharmacol,2019,112:42-53.
[30]	赵永伟,牛玉,何进田,等.双氢青蒿素对脂多糖诱导的断奶仔猪肝氧化应激的影响[J].畜牧兽医学报,2019, 50(10):2139-2146.ZHAO Y W,NIU Y,HE J T,et al.Effects of dihydroartemisinin on oxidative stress in liver of weaned piglets induced by lipopolysaccharide[J].Acta Veterinaria et Zootechnica Sinica,2019,50(10):2139-2146.(in Chinese)
[31]	LI S T,DAI Q,ZHANG S X,et al.Ulinastatin attenuates LPS-induced inflammation in mouse macro-phage RAW264.7 cells by inhibiting the JNK/NF-κB signaling pathway and activating the PI3K/Akt/Nrf2 pathway[J].Acta Pharmacol Sin,2018, 39(8):1294-1304.
[32]	LIN Y H,LIN Y J,CHANG T H,et al.Pipoxolan suppresses the inflammatory factors of NF-κB,AP-1,and STATs,but activates the antioxidative factor Nrf2 in LPS-stimulated RAW 264.7 murine macro-phage cells[J].Environ Toxicol,2020,35(12):1352-1363.
[33]	FUNAKOSHI-TAGO M,NONAKA Y,TAGO K,et al.Pyrocatechol,a component of coffee,suppresses LPS-induced inflammatory responses by inhibiting NF-κB and activating Nrf2[J].Sci Rep,2020,10(1):2584.
[34]	KIM H N,PARK G H,PARK S B,et al.Sageretia thea inhibits inflammation through suppression of NF-κB and MAPK and activation of Nrf2/HO-1 signaling pathways in RAW264.7 cells[J].Am J Chin Med,2019,47(2):385-403.
[35]	NAIR S,DOH S T,CHAN J Y,et al.Regulatory potential for concerted modulation of Nrf2- and Nfkb1-mediated gene expression in inflammation and carcinogenesis[J].Br J Cancer,2008,99(12):2070-2082.
[36]	BINH H T,TRI N M,QUAN N H,et al.Antibacterial activities and chemical composition of essential oil of Blumea balsamifera (L.) DC.,distributed in Lamdong province,Vietnam[J].Dalat Univ J Sci,2020,10(2):3-13.
[37]	陈前袆,曹春月,陈伟,等.艾纳香油中有效成分的定性检测及含量测定[J].应用化工,2020,49(3):788-791.CHEN Q H,CAO C Y,CHEN W,et al.Qualitative test and content determination of active components in Blumea balsamifera oil[J].Applied Chemical Industry,2020,49(3):788-791.(in Chinese)
[38]	LI Y,LV O,ZHOU F G,et al.Linalool inhibits LPS-induced inflammation in BV2 microglia cells by activating Nrf2[J]. Neurochem Res,2015,40(7):1520-1525.

Effect of Blumea balsamifera (L.) DC Oil on NF-κB and Nrf2/HO-1 Signal Pathway

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 38

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SUN Wenli, WANG Haoqi, ZE Licuo, GAO Yufan, ZHANG Feifan, ZHANG Jian, DUAN Mengqi, SHANG Peng, QIANG Bayangzong. Polymorphism of Pro-Inflammatory Factors (IL-1β, IL-6, TNF-α) in Tibetan Pigs and Its Association Analysis with Immune Traits [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1958-1969.
[2]	YANG Xiaofeng, QIN Xiaowei, LÜ Lihua. Protective Effect of a Derivative of MNQ Against LPS-Induced Inflammatory Injury in Bovine Ovarian Follicular Granulosa Cells in Vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2032-2041.
[3]	DAI Fan, LIU Zhanyou, ZHANG Xuyang, LI Wu. Aconitate Decarboxylase 1 Could Regulate the Inflammatory Response Caused by BCG [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1696-1706.
[4]	CHEN Hong, RUAN Hongri, MA Tianwen, LI Yanan, MIAO Xue, YANG Wenyue, GAO Li, WEI Chengwei. The Mechanism of Puerarin Improving Cartilage Degeneration in PTOA Rats by Interfering with Oxidative Stress and Nrf2/HO-1 Pathway of Cartilage [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(9): 3951-3963.
[5]	FAN Lei, SHEN Yu, YOU Liuchao, TIAN Xinyu, LUO Hao, WANG Xin, ZHANG Tingting, SHEN Liuhong. Research Progress on Abnormal Glucose and Lipid Metabolism in Dairy Cows Induced by Lipopolysaccharide (LPS) [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 484-493.
[6]	MENG Meijuan, WANG Yan, HUO Ran, LI Xuerui, CHANG Guangjun, SHEN Xiangzhen. Effect of Inhibition of PERK on LPS Induced Autophagy in Bovine Mammary Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 351-360.
[7]	ZHAO Xin, WANG Ying, LI Chunting, WANG Wei, SANG Rui, LI Haitao, ZHANG Xuemei. Alleviating Effect and Mechanism of Dandelion Extract on LPS-Induced Mastitis in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2773-2781.
[8]	LIU Xu, PAN Yinchuan, YANG Yajun, LIU Xiwang, MA Ning, LI Jianyong. Inhibiting Effect of Aspirin Eugenol Ester on Lipopolysaccharide-induced Inflammatory Response in Mouse Macrophages in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(11): 4048-4057.
[9]	LUO Jia, XU Jinrui, LI Wu, WANG Yujiong. The Effect of BCG-induced RAW264.7 Cells Fatty Acid Oxidation on Autophagy and Pro-inflammatory Cytokines Expression [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2617-2625.
[10]	HE Shangwen, WANG Yueming, ZHANG Hui, HOU Silu, LIU Xiaoye, DONG Hong. Pulsatilla Decoction Alleviates LPS-induced Inflammation of Microvascular Endothelial Cells by Inhibiting the TLR4-ERK1/2 Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(8): 2317-2325.
[11]	ZHANG Haiyang, LU Xiangyu, ZHANG Jiahua, LIU Xin, HAN Zhengxuan, CUI An, ZHAO Yuan, SONG Manyu, LIU Tao, FAN Honggang. Protective Effects of Melatonin on LPS-induced Hippocampal Inflammatory Lesion in Rats [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 226-234.
[12]	CHEN Kaiwen, HUA Chengwei, YUAN Chen, PAN Fei, LIN Huixing, FAN Hongjie, MA Zhe. Effects of Enolase of Streptococcus equi ssp. zooepidemicus on Phagocytic Functions of Alveolar Macrophages in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(10): 2576-2583.
[13]	SHAO Dan, SONG Pengjie, YAN Baoqi, WU Xiaohu, WANG Dongsheng, LI Yajuan, ZHANG Shidong, YAN Zuoting. Inhibiting Effect of Thymol on Inflammation Induced by LPS in Endometrial Epithelial Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(7): 1493-1499.
[14]	PENG Zhong, LIANG Wan, AI Weicheng, WANG Fei, HUA Lin, TANG Xibiao, CHEN Huanchun, WU Bin. Genotypical Characteristics of Swine Pasteurella multocida in China [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(5): 1064-1072.
[15]	HE Shuangjiang, SONG Ruilong, CAO Ying, LIU Qingyang, ZHANG Chuang, LIU Zongping. Effects of Low Level Cadmium Exposure on Osteoclast Differentiation [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(3): 663-669.