艾纳香油对NF-κB及Nrf2/HO-1信号通路的作用研究

doi:10.11843/j.issn.0366-6964.2021.04.013

Abstract

Abstract: The purpose of this study was to reveal the mechanism of anti-inflammatory effect of BBO by studying NF-κB and Nrf2/HO-1 pathway. RAW264.7 macrophage inflammation model induced by LPS was used. The control group, LPS model group, BBO group (low, middle and high dose) and BBO negative control group were set up in this experiment, with 3 repeats in each group. The effect of BBO on apoptosis was detected by Hoechst 33342 and PI double staining. The contents of IL-1β, TNF-α, PGE₂, LTB₄, NO and the activity of iNOS in LPS-induced macro-phage were detected by ELISA and spectrophotometry. The effect of BBO on the mRNA expression of TNF-α and IL-1β was detected by RT-PCR. The effect of BBO on the key proteins expression in NF-κB and Nrf2/HO-1 signal pathway was detected by Western blotting. The results showed that BBO could reverse the morphological changes and apoptosis of macrophages induced by LPS within the dose range of 80 μg·mL^-1. Compared with LPS model group, BBO of 60-80 μg·mL^-1 could extremely significantly inhibit the secretion of LPS-induced cellular inflammatory factors and inflammatory mediators IL-1β, TNF-α, PGE₂, LTB₄, NO and iNOS activity(P<0.01); BBO could also extremely significantly inhibit the expression of IL-1β and TNF-α mRNA(P<0.01) induced by LPS. At the same time, BBO of 60-80 μg·mL^-1 could extremely significantly down-regulate the protein expression of COX-2, 5-LOX, p-IKKα, p-P65, p-IκB-α and cytoplasmic Nrf2 induced by LPS(P<0.01), and extremely significantly promote the protein expression of HO-1 and nuclear Nrf2 in a dose-dependent manner(P<0.01). The results suggest that BBO has good effects on anti-inflammatory and anti-apoptotic. It may play an anti-inflammatory role by inhibiting the phosphorylation of key proteins and the production of inflammatory factors in NF-κB pathway and promote the expression of major anti-inflammatory genes in Nrf2/HO-1 pathway.

Key words: Blumea balsamifera(L.) DC Oil, LPS, RAW264.7 cell, NF-κB, Nrf2/HO-1 pathway

CLC Number:

S852.2

WANG Wanlin, GAO Yue, LIAO Jiamei, PENG Junchao, CAI Yaling, YI Qiong, WANG Lu. Effect of Blumea balsamifera (L.) DC Oil on NF-κB and Nrf2/HO-1 Signal Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(4): 976-986.

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Effect of Blumea balsamifera (L.) DC Oil on NF-κB and Nrf2/HO-1 Signal Pathway

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