中国禽传染性支气管炎病毒的遗传演化及时空传播分析

doi:10.11843/j.issn.0366-6964.2023.06.032

Abstract

Abstract: The aim of this study was to explore the rules of the genetic variation and spatio-temporal transmission of the Chinese IBV isolates, all the sequences of N gene from GenBank database were downloaded. Bioinformatic software including MEGA 6.0, RDP 4.95, SimPlot 3.5.1, BEAST v1.10.4, jmodeltest 2.1.7, Tracer v1.7.1, TempEst v1.5.1, FigTree v1.4.3 and SpreaD3 v0.9.7 were respectively used to analyze the characteristics of phylogenetic tree, recombination events, origin,population dynamics and spatio-temporal transmission of the IBV isolates. The phylogenetic tree analysis showed that a total of six genotypes were identified, and the LX4-type was the predominant genotype. The recombination analysis showed that recombination event was found in N gene in one of isolate from Sichuan province. The maximum clade credibi-lity tree showed that the Chinese IBVs were most likely to be originated in Liaoning province in the early 1930s. Bayesian phylogeographic analyses indicated that multiple transmission routes and three epicenters in China were found, including the Northeastern Region (Heilongjiang, Liaoning, and Jilin), the Northern and Eastern Region (Hebei, Shandong and Jiangsu) and the Southern Region (Guangdong and Guangxi). Shandong has been the source of spreads in China. Our study revealed that the IBVs' origin, transmission routes and a recombination event occurred in N gene in China, suggesting that it is necessary to continue to carry out the molecular epidemiology study and strengthen the analysis of the N gene of IBV.

Key words: infectious bronchitis virus, genetic variation, recombination, origin, spatial transmission

CLC Number:

S851.31

FAN Wensheng, LIU Sijia, QIU Shenben, HUANG Aifang, WANG Yan, LIU Minfang, MEI Minmin, CHEN Xinliang, WEI Ping, MO Meilan. Genetic Evolution and Spatio-temporal Transmission Analysis of the Chinese Infectious Bronchitis Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2543-2554.

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Genetic Evolution and Spatio-temporal Transmission Analysis of the Chinese Infectious Bronchitis Virus

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